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| Registros recuperados : 21 | |
| 2. |  | ROSA, L. H.; QUEIROZ, S. C. do N. de; WANG, X.; TECHEN, N.; CANTRELL, C. L.; WEDGE, D. E. Antifungal fatty acids produced by Coniochaeta ligniaria: a cryptic endophytic fungus associated with Smallanthus sonchifolius' autotrophic cultures. In: Planta Medica, Dordrecht, v. 79, n. 5, p. 5, 2013. Abstracts of the 12th International Conference on the Science of Botanicals (ICBS), Oxford, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 3. | | CAMPAGNANI, M. O.; CAMPOS, W. G.; AMORIM, S. S.; ROSA, L. H.; AUAD, A. M.; MAURÍCIO, M. Bioprospecção de fungos entomopatogênicos associados à Mahanarva spectabilis na Amazônia In: CONGRESSO BRASILEIRO DE MICOLOGIA, 8., 2016, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Micologia, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | GONÇALVES, V. N.; CAMPOS, L. S.; MELO, I. S. de; PELLIZARI, V. H.; ROSA, C. A.; ROSA, L. H. Penicillium solitum: a mesophilic, psychrotolerant fungus present in marine sediments from Antarctica. Polar Biology, v. 36, n. 12, p. 1823-1831, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | MENDONÇA, M. C. de; AUAD, A. M.; MADUREIRA, A. P.; MAURÍCIO, R. M.; SILVA, N. B. N.; SILVA, A. C. C. R.; ROSA, L. H.; CAMPOS, W. G. Inoculação endofítica de fungos entomopatogênicos em Brassica oleracea var. acephala L. para o controle biológico de Plutella xylostella (Lepdoptera) e Brevicoryne brassicae (Hemiptera). In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 32., 2023, Foz do Iguaçu. Resumos. São Paulo: Sociedade Brasileira de Microbiologia, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | MELO, I. S. de; SANTOS, S. N.; ROSA, L. H.; PARMA, M. M.; SILVA, L. J.; QUEIROZ, S. C. do N. de; PELLIZARI, V. H. Isolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici. Extremophiles, Tokyo, v. 18, n. 1, p. 15-23, 2014.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | MELO, I. S. de; ZUCCHI, T. D.; SILVA, R. E. da; VILELA, E. S. D.; SÁBER, M. L.; ROSA, L. H.; PELLIZARI, V. H. Isolation and characterization of cellulolytic bacteria from the Stain house Lake, Antarctica. Folia Microbiologica, Prague, v. 59, p. 303-306, 2014.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 9. | | SILVA, L. J. da; SOUZA, D. T.; GENUÁRIO, D. B.; VARGAS-HOYOS, H. A.; SANTOS, S. N.; ROSA, L. H.; ZUCCHI, T. D.; MELO, I. S. de. Rhodococcus psychrotolerans sp. nov., isolated from rhizosphere of Deschampsia antarctica. Antonie van Leeuwenhoek, v. 111, n. 4, p. 629-636, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 10. | | MELO, I. S. de; VASCONCELLOS, R. L. F.; NASCIMENTO, R. dos S.; VILELA, E. S. D.; KAVAMURA, V. N.; ROSA, L. H. Endophytic bacteria from Deschampsia antarctica and assessment of their potential to promote plant growth. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 15., 2014, Seoul. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2014. p. 139-140.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | ROSA, L. H.; QUEIROZ, S. C. do N. de; MORAES, R. M.; WANG, X.; TECHEN, N.; PAN, Z.; CANTRELL, C. L.; WEDGE, D. E. Coniochaeta ligniaria: antifungal activity of the cryptic endophytic fungus associated with autotrophic tissue cultures of the medicinal plant Smallanthus sonchifolius (Asteraceae). Symbiosis, Philadelphia, v. 60, p. 133-142, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | CAMPAGNANI, M. O.; CAMPOS, W. G.; AMORIM, S. S.; ROSA, L. H.; AUAD, A. M.; CANGUSSÚ, M. A.; MAURÍCIO, R. M. Prospection and fungal virulence associated with Mahanarva spectabilis (Hemiptera: Cercopidae) in an Amazon silvopastoral system. Florida Entomologist, v. 100, n. 2, p. 426-432, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | MELO, I. S. de; VILELA, E. S. D.; PARMA, M. M.; NASCIMENTO, R. dos S.; ROSA, L. H.; PELLIZARI, V. H. Bactérias celulolíticas isoladas do lago Stenhouse na Península Antártica. In: WORKSHOP DE BIODEGRADAÇÃO E BIORREMEDIAÇÃO, 3., 2009, Campinas. Anais... Jaguariúna: Embrapa Meio Ambiente, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 14. | | CAMPAGNANI, M. O.; MADUREIRA, A. P.; VIANA, J. P. R.; AMORIM, S. S.; ROSA, L. H.; AUAD, A. M.; CANGUSSU, M. A.; MAURICIO, R. M.; CAMPOS, W. G. Virulência em lagartas (lepidoptera) de novos fungos entomopatogênicos isolados de Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae). In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 5., 2018, Belo Horizonte. Resumos... Belo Horizonte: Universidade Federal de Minas Gerais, 2018. p. 93.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | CAMPAGNANI, M. O.; MADUREIRA, A. P.; OLIVEIRA, M. M. B.; MAGALHÃES SILVA, N. B.; RIOS SILVA, A. C.; COELHO, L. C.; ROSA, L. H.; AUAD, A. M.; CANGUSSÚ, M. A.; MAURÍCIO, R. M.; CAMPOS, W. G. Eficiência de fungos entomopatogênicos contra Mahanarva spectabilis (distant) (hemiptera: cercopidae) a campo. In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 5., 2018, Belo Horizonte. Resumos... Belo Horizonte: Universidade Federal de Minas Gerais, 2018. p. 94.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | MENDONÇA, M. C. de; AUAD, A. M.; MADUREIRA, A. P.; MAURÍCIO, R. M.; CANGUSSU, M.; ROSA, L. H.; PEREIRA, M. F.; MUNIZ, M.; SOUZA, S. R. O.; SILVA, A. C. R.; SILVA, N. B. N.; CAMPOS, W. G. Eficiência de fungos isolados em sistema silvipastoril no controle biológico de Mahanarva spectabilis (hemiptera: cercopidae) em condições de campo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 32., 2023, Foz do Iguaçu. Resumos. São Paulo: Sociedade Brasileira de Microbiologia, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | OKI, Y.; GOTO, B. T.; JOBIM, K.; ROSA, L. H.; FERREIRA, M. C.; COUTINHO, E. S.; XAVIER, J. H. de A.; CARVALHO, F.; MOREIRA, F. M. de S.; SOUZA, F. A. de; BERBARA, R. L. L.; FERNANDES, G. W. Arbuscular mycorrhiza and endophytic fungi in ruspestrian grasslands. In: FERNANDES, G. W. (Ed.). Ecology and conservation of mountaintop grasslands in Brazil. Switzerland: Springer International Publishing, 2016. cap. 8, p. 157-179.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | SILVA, L. J. da; CREVELIN, E. J.; SOUZA, D. T.; LACERDA JÚNIOR, G. V.; OLIVEIRA, V. M.; RUIZ, A. L. T. G.; ROSA, L. H.; MORAES, L. A. B.; MELO, I. S. de. Actinobacteria from Antarctica as a source for anticancer discovery. Scientific Reports, v. 10, article 13870, 2020. p. 1-15.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | CARVALHO, C. R. de; MAIA, M. Q.; SOBRAL, M.; PEREIRA, G. M. D.; SILVA, K. da; VITAL, M. J. S.; ZILLI, J. E.; ROSA, C. A.; ROSA, L. H. Diversity and antimicrobial activity of culturable endophytic fungi associated with the neotropical ethnomedicinal plants Copaifera langsdorffii and Copaifera pubiflora South African Journal of Botany, v. 142, p. 305-315, Nov. 2021. 11 p.| Biblioteca(s): Embrapa Agrobiologia; Embrapa Florestas. |
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| 20. | | CARVALHO, C. R. de; MAIA, M. Q.; SOBRAL, M.; PEREIRA, G. M. D.; SILVA, K. da; VITL, M. J. S.; ZILLI, J. E.; ROSA, C. A.; ROSA, L. H. Diversity and antimicrobial activity of culturable endophytic fungi associated with the neotropical ethnomedicinal plants Copaifera langsdorffii and Copaifera pubiflora. South African Journal of Botany, v. 142, p. 305-331, November 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 21 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. J.; DUARTE, A. W. F.; ROSA, L. H.; OLIVEIRA, V. M.; MELO, I. S. de. |
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Afiliação: |
L. J. SILVA, ESALQ/USP; A. W. F. DUARTE, USP; L. H. ROSA, UFMG; V. M. OLIVEIRA, CPQBA/Unicamp. |
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Título: |
Fosmid library: methodological challenges. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest. MenosAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A p... Mostrar Tudo |
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Palavras-Chave: |
Antarctic peninsula; Metagenomic library; Unculturable microrganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139515/1/2015RA-008.pdf
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|
Marc: |
LEADER 03101nam a2200193 a 4500
001 2037800
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, L. J.
245 $aFosmid library$bmethodological challenges.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1.$c0676
520 $aAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest.
653 $aAntarctic peninsula
653 $aMetagenomic library
653 $aUnculturable microrganisms
700 1 $aDUARTE, A. W. F.
700 1 $aROSA, L. H.
700 1 $aOLIVEIRA, V. M.
700 1 $aMELO, I. S. de
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