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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
27/03/2009 |
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Data da última atualização: |
08/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRAND, G. D.; MAGALHÃES, M. T. Q. de; BAMBIRRA, F. H. S.; KELLY, S. M.; NICOLI, J. R.; COOPER, A; BLOCH JUNIOR, C. |
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Afiliação: |
GUILHERME DOTTO BRAND; MARIANA TORQUATO QUEZADA DE MAGALHÃES; FELIPE HENRIQUE S. BAMBIRRA; SHARON M. KELLY, UNIVERSITY OF GLASGOW; JACQUES R. NICOLI, UFMG; ALAN COOPER, UNIVERSITY OF GLASGOW; CARLOS BLOCH JUNIOR, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
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Título: |
Functional categorization of membrane-active peptides. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Thesagro: |
Peptídeo. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00675nam a2200205 a 4500
001 1191199
005 2024-05-08
008 2008 bl uuuu u01u1 u #d
100 1 $aBRAND, G. D.
245 $aFunctional categorization of membrane-active peptides.$h[electronic resource]
260 $aIn: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq$c2008
300 $c1 CD-ROM.
650 $amolecular biology
650 $aPeptídeo
700 1 $aMAGALHÃES, M. T. Q. de
700 1 $aBAMBIRRA, F. H. S.
700 1 $aKELLY, S. M.
700 1 $aNICOLI, J. R.
700 1 $aCOOPER, A
700 1 $aBLOCH JUNIOR, C.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
14/10/2020 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Poultry Science, v. 99, n. 11, 2020. |
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Páginas: |
6247-6257 |
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DOI: |
https://doi.org/10.1016/j.psj.2020.06.066 |
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Idioma: |
Inglês |
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Conteúdo: |
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. MenosMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transvers... Mostrar Tudo |
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Palavras-Chave: |
Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03360naa a2200361 a 4500
001 2125497
005 2022-08-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.psj.2020.06.066$2DOI
100 1 $aCÔNSOLO, N. R. B.
245 $aCharacterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry$ba pilot study.$h[electronic resource]
260 $c2020
300 $a6247-6257
520 $aMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry.
653 $aChicken breast myopathy
653 $aNMR spectroscopy
653 $aTD-NMR relaxometry
653 $aWater mobilization
700 1 $aSAMUELSSON, L. M.
700 1 $aBARBOSA, L. C. G. S.
700 1 $aMONARETTO, T.
700 1 $aMORAES, T. B.
700 1 $aBUARQUE, V. L. M.
700 1 $aHIGUERA-PADILLA, A. R.
700 1 $aCOLNAGO, L. A.
700 1 $aSILVA, S. L.
700 1 $aREIS, M. M.
700 1 $aFONSECA, A. C.
700 1 $aARAÚJO, C. S. S.
700 1 $aLEITE, B. G. S.
700 1 $aROQUE, F. A.
700 1 $aARAÚJO, L. F.
773 $tPoultry Science$gv. 99, n. 11, 2020.
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