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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/05/2013 |
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Data da última atualização: |
02/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTELANI, L.; SANTOS, A. F. S.; MIRANDA, M. dos S.; ZAFALON, L. F.; POZZI, C. R.; ARCARO, J. R. P. |
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Afiliação: |
LÍVIA CASTELANI, Instituto de Zootecnia; ALINE FRANCIELE SILVA SANTOS, Instituto de Zootecnia; MARIANA DOS SANTOS MIRANDA, Instituto de Zootecnia; LUIZ FRANCISCO ZAFALON, CPPSE; CLAUDIA RODRIGUES POZZI, Instituto de Zootecnia; JULIANA RODRIGUES POZZI ARCARO, Instituto de Zootecnia. |
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Título: |
Molecular typing of mastitis-causing Staphylococcus aureus isolated from heifers and cows. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
International Journal of Molecular Sciences, v. 14, p. 4326-4333, 2013. |
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DOI: |
10.3390/ijms14024326 |
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Idioma: |
Português |
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Conteúdo: |
Staphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State, Brazil. The highest frequency of S. aureus isolation in heifers was at the end of lactation. Strains were typified through Pulsed-field gel electrophoresis (PFGE) and grouped according to patterns of restriction enzyme SmaI. PFGE generated seven clonal profiles that were grouped into three different lineages, with the LA lineage being predominant and identified in heifers, as well as in the cows from the two regions studied. It was concluded that the cows showed a significant source of dispersion of S. aureus. At the first lactation the heifers were infected by the same clonal profiles of S. aureus which were isolated from multiparous lactating cows. The heifers were infected during milking over the months of lactation. |
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Palavras-Chave: |
PFGE. |
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Thesaurus Nal: |
epidemiology; heifers. |
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Categoria do assunto: |
G Melhoramento Genético
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95367/1/PROCI-2013.000017.PDF
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Marc: |
LEADER 01812naa a2200229 a 4500
001 1959003
005 2023-05-02
008 2013 bl uuuu u00u1 u #d
024 7 $a10.3390/ijms14024326$2DOI
100 1 $aCASTELANI, L.
245 $aMolecular typing of mastitis-causing Staphylococcus aureus isolated from heifers and cows.$h[electronic resource]
260 $c2013
520 $aStaphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State, Brazil. The highest frequency of S. aureus isolation in heifers was at the end of lactation. Strains were typified through Pulsed-field gel electrophoresis (PFGE) and grouped according to patterns of restriction enzyme SmaI. PFGE generated seven clonal profiles that were grouped into three different lineages, with the LA lineage being predominant and identified in heifers, as well as in the cows from the two regions studied. It was concluded that the cows showed a significant source of dispersion of S. aureus. At the first lactation the heifers were infected by the same clonal profiles of S. aureus which were isolated from multiparous lactating cows. The heifers were infected during milking over the months of lactation.
650 $aepidemiology
650 $aheifers
653 $aPFGE
700 1 $aSANTOS, A. F. S.
700 1 $aMIRANDA, M. dos S.
700 1 $aZAFALON, L. F.
700 1 $aPOZZI, C. R.
700 1 $aARCARO, J. R. P.
773 $tInternational Journal of Molecular Sciences$gv. 14, p. 4326-4333, 2013.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
29/03/2016 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
HELENE, L. C. F.; DELAMUTA, J. R. M.; RIBEIRO. R. A.; ORMEÑO-ORRILLO, E.; ROGEL, M. A; MARTINEZ-ROMERO, E.; HUNGRIA, M. |
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Afiliação: |
Luisa Caroline Ferraz Helene, UEL; Jakeline Renata Marçon Delamuta, UEL; Renan Augusto Ribeiro, Conselho Nacional de Desenvolvimento Cientifico e Tecnológico; Ernesto Ormeño-Orrillo, Universidad Nacional Agraria La Molina; Marco Antonio Rogel, Centro de Ciencias Genó micas, Universidad Nacional Autónoma de México; Esperanza Martinez-Romero, Centro de Ciências Genómicas, Universidad Nacional Autónoma de México; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Reading v. 65, n. 12, 2015. |
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Páginas: |
p. 4441-4438 |
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Idioma: |
Português |
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Conteúdo: |
Symbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were determined. Our data supported the description of the SEMIA strains as Bradyrhizobium viridifuturi sp. nov., and SEMIA 690T (5CNPSo 991T5C 100aT5BR 1804T5LMG 28866T), isolated from Centrosema pubescens, was chosen as type strain. MenosSymbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were det... Mostrar Tudo |
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Palavras-Chave: |
Fixação biologica de nitrogenio. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02480naa a2200217 a 4500
001 2041960
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
100 1 $aHELENE, L. C. F.
245 $aBradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services.$h[electronic resource]
260 $c2015
300 $ap. 4441-4438
520 $aSymbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were determined. Our data supported the description of the SEMIA strains as Bradyrhizobium viridifuturi sp. nov., and SEMIA 690T (5CNPSo 991T5C 100aT5BR 1804T5LMG 28866T), isolated from Centrosema pubescens, was chosen as type strain.
653 $aFixação biologica de nitrogenio
700 1 $aDELAMUTA, J. R. M.
700 1 $aRIBEIRO. R. A.
700 1 $aORMEÑO-ORRILLO, E.
700 1 $aROGEL, M. A
700 1 $aMARTINEZ-ROMERO, E.
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, Reading$gv. 65, n. 12, 2015.
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