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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/04/2024 |
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Data da última atualização: |
18/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, E. R. dos; CAMARGO, B. R. de; SILVA, L. A. da; LAUMANN, R. A.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P. |
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Afiliação: |
ETHIANE ROZO DOS SANTOS, UNIVERSIDADE DE BRASÍLIA; BRENDA RABELO DE CAMARGO, UNIVERSIDADE DE BRASÍLIA; LEONARDO ASSIS DA SILVA, UNIVERSIDADE DE BRASÍLIA; RAUL ALBERTO LAUMANN, CENARGEN; BERGMANN MORAIS RIBEIRO, UNIVERSIDADE DE BRASÍLIA; DANIEL M. P. ARDISSON-ARAÚJO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, 2024. |
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DOI: |
https://doi.org/10.1007/s42770-024-01340-y |
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Idioma: |
Português |
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Notas: |
On-line first. |
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Conteúdo: |
Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. MenosWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological si... Mostrar Tudo |
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Palavras-Chave: |
Egg parasitoid; Halyomorpha halys virus; Stinkbug; Telenomus podisi. |
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Categoria do assunto: |
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Marc: |
LEADER 02558naa a2200253 a 4500
001 2163741
005 2024-04-18
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-024-01340-y$2DOI
100 1 $aSANTOS, E. R. dos
245 $aThe multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera$bPlatygastridae).$h[electronic resource]
260 $c2024
500 $aOn-line first.
520 $aWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector.
653 $aEgg parasitoid
653 $aHalyomorpha halys virus
653 $aStinkbug
653 $aTelenomus podisi
700 1 $aCAMARGO, B. R. de
700 1 $aSILVA, L. A. da
700 1 $aLAUMANN, R. A.
700 1 $aRIBEIRO, B. M.
700 1 $aARDISSON-ARAÚJO, D. M. P.
773 $tBrazilian Journal of Microbiology, 2024.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FONGARO, G.; GARCÍA-GONZALEZ, M. C.; HERNANDEZ, M.; KUNZ, A.; BARARDI, C. R. M.; RODRIGUEZ-LÁZARO, D. |
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Afiliação: |
GISLAINE FONGARO, UFSC; MARIA C. GARCÍA-GONZALEZ, Instituto Tecnológico Agrario de Castilla y León; MARTA HERNANDEZ, Universidad de Valladolid, Palencia; AIRTON KUNZ, CNPSA; CÉLIA REGINA M. BARARDI, UFSC; DAVID RODRIGUEZ-LÁZARO, Universidad de Burgos. |
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Título: |
Different behavior of enteric bacteria and viruses in cay and sandy soils after biofertilization with swine digestate. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v. 8, p. 1-9, 2017. |
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DOI: |
10.3389/fmicb.2017.00074 |
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Idioma: |
Inglês |
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Conteúdo: |
Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174 in clay soil was significantly lower than in sandy soil (??T90 values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12.040 ± 0.010 vs. 43.470 ± 1.300, respectively) and PhiX- 174 showed faster percolation and leaching in sandy soil than clay soil (??T90 values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (??T90 values of 9.340 ± 0.200 vs. 6.620 ± 0.500 and 11.900 ± 0.900 vs. 10.750 ± 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recycling MenosEnteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174 in clay soil was significantly lower than in sandy soil (??T90 values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12.040 ± 0.010 vs. 43.470 ± 1.300, respectively) and PhiX- 174 showed faster percolation and leaching in sandy soil than clay soil (??T90 values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (??T90 values of 9.340 ± 0.200 vs. 6.620 ± 0.500 and 11.900 ± 0.900 vs. 10.750 ± 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recyc... Mostrar Tudo |
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Thesagro: |
Biofertilizante; Contaminação; Dejeto; Manejo do solo; Meio ambiente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02280naa a2200253 a 4500
001 2081333
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2017.00074$2DOI
100 1 $aFONGARO, G.
245 $aDifferent behavior of enteric bacteria and viruses in cay and sandy soils after biofertilization with swine digestate.$h[electronic resource]
260 $c2017
520 $aEnteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174 in clay soil was significantly lower than in sandy soil (??T90 values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12.040 ± 0.010 vs. 43.470 ± 1.300, respectively) and PhiX- 174 showed faster percolation and leaching in sandy soil than clay soil (??T90 values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (??T90 values of 9.340 ± 0.200 vs. 6.620 ± 0.500 and 11.900 ± 0.900 vs. 10.750 ± 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recycling
650 $aBiofertilizante
650 $aContaminação
650 $aDejeto
650 $aManejo do solo
650 $aMeio ambiente
700 1 $aGARCÍA-GONZALEZ, M. C.
700 1 $aHERNANDEZ, M.
700 1 $aKUNZ, A.
700 1 $aBARARDI, C. R. M.
700 1 $aRODRIGUEZ-LÁZARO, D.
773 $tFrontiers in Microbiology$gv. 8, p. 1-9, 2017.
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