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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
26/05/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PETRILLO, C. P.; CARNEIRO, N. P.; PURCINO, A. A. C.; CARVALHO, C. H. S.; ALVES, J. D.; CARNEIRO, A. A. |
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Afiliação: |
Cristiane Pereira Petrillo, UFLA; NEWTON PORTILHO CARNEIRO, CNPMS; ANTONIO ALVARO CORSETTI PURCINO, CNPMS; Carlos Henrique Siqueira Carvalho, Embrapa Café; José Donizeti Alves, UFLA; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v. 43, n. 3, p. 371-378, mar. 2008. |
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DOI: |
10.1590/S0100-204X2008000300012 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics. RESUMO: O objetivo deste trabalho foi desenvolver um sistema de transformação genética para genótipos tropicais de milho pelo bombardeamento de embriões imaturos com micropartículas. O bombardeamento com partículas foi realizado utilizando-se uma construção gênica contendo os genes bar e uidA sob o controle do promotor CaMV35S. As melhores condições para transformação das linhagens de milho tropical L3 e L1345 foram obtidas após o cultivo de embriões imaturos durante 4 horas, antes do bombardeamento, em alta osmolaridade, e bombardeados com a pressão de 1.100 psi de gás hélio, dois tiros por placa e uma distância de vôo da micropartícula de 6,6 cm. As freqüências de transformação obtidas variaram entre 0,9 e 2,31%. A integração dos genes heterólogos no genoma das plantas transgênicas de milho foi confirmada por análises de Southern blot e expressão dos genes bar e uidA. O protocolo de transformação genética de milho desenvolvido neste estudo irá aumentar, provavelmente, a eficiência de produção de novas linhagens tropicais de milho expressando características agronômicas desejáveis. MenosABSTRACT: The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics. RESUMO: O objetivo deste trabalho foi desenvolver um sistema de transformação genética para genótipos tropicais de milho pelo bombardeamento de embriões imaturos com micropartículas. O bombardeamento com partículas foi realizado utilizando-se uma construção gênica contendo os genes bar e uidA sob o controle do promotor CaMV35S. As melhores condições para transformação das linhagens de milho tropical L3 e L1345 foram obtidas após o cultivo de embriões imaturo... Mostrar Tudo |
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Palavras-Chave: |
ammonium glufosinate; embriões imaturos; fosfinotricina acetil transferase; glufosinato de amônio; immature embryos; phosphinotricin acetyl transferase; plant transformation; transformação de plantas. |
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Thesagro: |
Milho; Zea Mays. |
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Thesaurus Nal: |
beta-glucuronidase. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160678/1/Optimization-particle.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/43238/1/43n03a12.pdf
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Marc: |
LEADER 03218naa a2200325 a 4500
001 1491013
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2008000300012$2DOI
100 1 $aPETRILLO, C. P.
245 $aOptimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines.
260 $c2008
520 $aABSTRACT: The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics. RESUMO: O objetivo deste trabalho foi desenvolver um sistema de transformação genética para genótipos tropicais de milho pelo bombardeamento de embriões imaturos com micropartículas. O bombardeamento com partículas foi realizado utilizando-se uma construção gênica contendo os genes bar e uidA sob o controle do promotor CaMV35S. As melhores condições para transformação das linhagens de milho tropical L3 e L1345 foram obtidas após o cultivo de embriões imaturos durante 4 horas, antes do bombardeamento, em alta osmolaridade, e bombardeados com a pressão de 1.100 psi de gás hélio, dois tiros por placa e uma distância de vôo da micropartícula de 6,6 cm. As freqüências de transformação obtidas variaram entre 0,9 e 2,31%. A integração dos genes heterólogos no genoma das plantas transgênicas de milho foi confirmada por análises de Southern blot e expressão dos genes bar e uidA. O protocolo de transformação genética de milho desenvolvido neste estudo irá aumentar, provavelmente, a eficiência de produção de novas linhagens tropicais de milho expressando características agronômicas desejáveis.
650 $abeta-glucuronidase
650 $aMilho
650 $aZea Mays
653 $aammonium glufosinate
653 $aembriões imaturos
653 $afosfinotricina acetil transferase
653 $aglufosinato de amônio
653 $aimmature embryos
653 $aphosphinotricin acetyl transferase
653 $aplant transformation
653 $atransformação de plantas
700 1 $aCARNEIRO, N. P.
700 1 $aPURCINO, A. A. C.
700 1 $aCARVALHO, C. H. S.
700 1 $aALVES, J. D.
700 1 $aCARNEIRO, A. A.
773 $tPesquisa Agropecuária Brasileira, Brasília$gv. 43, n. 3, p. 371-378, mar. 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
04/07/2022 |
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Data da última atualização: |
04/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VALICENTE, F. H.; DANTAS, C. L.; RESENDE, J. P. V.; PAIVA, P. M. de; SOUZA, C. F. de; NASCIMENTO, P. T.; OLIVEIRA, C. R. de; BOREGAS, K. G. B.; RODRIGUEZ-DIMATÉ, F. A.; AGUIAR, R. M. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; CAIO LEÃO DANTAS; JOAQUIM PEDRO VIEIRA RESENDE; PRISCILA MARQUES DE PAIVA; CAMILA FERNANDES DE SOUZA; PRISCILLA TAVARES NASCIMENTO; CLEIDIANE RODRIGUES DE OLIVEIRA; KÁTIA GISELE BRASIL BOREGAS; FRANCISCO ANDRÉS RODRIGUEZ-DIMATÉ, Universidade Federal de Viçosa; FREDERICK MENDES AGUIAR. |
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Título: |
A 6-year field monitoring of fall armyworm, Spodoptera frugiperda, in transgenic Bt maize in Brazil. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Revista Brasileira de Entomologia, v. 66, n. 2, e20210121, 2022. |
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DOI: |
https://doi.org/10.1590/1806-9665-RBENT-2021-0121 |
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Idioma: |
Inglês |
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Conteúdo: |
A 6-year field monitoring study was designed to compare the presence of fall armyworm Spodoptera frugiperda (J. E. Smith), leaf injury, its parasitoids, 100 seed weight, and seed yield of Bt maize hybrids approved for commercialization in the Brazilian market. Field trials were planted in two municipalities, divided into two periods, from 2011 to 2014 (period I), and due to the approval of different Bt hybrids, from 2015 to 2016 (period II). Treatments were Bt, non-Bt maize and all non-Bt maize sprayed with insecticide methomyl. Six Bt-maize hybrids were planted, expressing the following proteins: Cry1Ab, Cry1F, Cry1A.105 (Cry1Ab +Cry1Ac +Cry1F), Cry2Ab2a and Vip3. In 2015 and 2016 Bt Powercore was planted due to its commercial approval. The number of S. frugiperda larvae for most Bt hybrids was lower than on non-Bt hybrids, except for Fórmula TL and Herculex in period II, in both municipalities. In period I, Bt hybrids VT, VT 2 and Viptera showed almost no injury on their leaves, despite the high number of fall armyworm larvae found in plants. Different parasitoids emerged from larvae from Bt and non Bt hybrids: Archytas sp., Campoletis sp., Chelonus sp., Eiphosoma sp. and Ophion luteus. The average 100 seed weight was higher in municipality from Sete Lagoas for all Bt hybrids, non-Bt hybrids and non-Bt hybrids sprayed with methomyl, when compared to Nova Porteirinha during period I. Fórmula TL, non-Bt Fórmula TL sprayed with methomyl, and non-Bt Fórmula TL showed the lowest seed yield in both municipalities and periods. MenosA 6-year field monitoring study was designed to compare the presence of fall armyworm Spodoptera frugiperda (J. E. Smith), leaf injury, its parasitoids, 100 seed weight, and seed yield of Bt maize hybrids approved for commercialization in the Brazilian market. Field trials were planted in two municipalities, divided into two periods, from 2011 to 2014 (period I), and due to the approval of different Bt hybrids, from 2015 to 2016 (period II). Treatments were Bt, non-Bt maize and all non-Bt maize sprayed with insecticide methomyl. Six Bt-maize hybrids were planted, expressing the following proteins: Cry1Ab, Cry1F, Cry1A.105 (Cry1Ab +Cry1Ac +Cry1F), Cry2Ab2a and Vip3. In 2015 and 2016 Bt Powercore was planted due to its commercial approval. The number of S. frugiperda larvae for most Bt hybrids was lower than on non-Bt hybrids, except for Fórmula TL and Herculex in period II, in both municipalities. In period I, Bt hybrids VT, VT 2 and Viptera showed almost no injury on their leaves, despite the high number of fall armyworm larvae found in plants. Different parasitoids emerged from larvae from Bt and non Bt hybrids: Archytas sp., Campoletis sp., Chelonus sp., Eiphosoma sp. and Ophion luteus. The average 100 seed weight was higher in municipality from Sete Lagoas for all Bt hybrids, non-Bt hybrids and non-Bt hybrids sprayed with methomyl, when compared to Nova Porteirinha during period I. Fórmula TL, non-Bt Fórmula TL sprayed with methomyl, and non-Bt Fórmula TL showed the lowes... Mostrar Tudo |
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Palavras-Chave: |
Bt; Lagarta-do-cartucho. |
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Thesagro: |
Alimento Transgênico; Bacillus Thuringiensis; Milho; Toxina. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144420/1/A-6-year-field-monitoring-of-fall-armyworm.pdf
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Marc: |
LEADER 02515naa a2200313 a 4500
001 2144420
005 2022-07-04
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1806-9665-RBENT-2021-0121$2DOI
100 1 $aVALICENTE, F. H.
245 $aA 6-year field monitoring of fall armyworm, Spodoptera frugiperda, in transgenic Bt maize in Brazil.$h[electronic resource]
260 $c2022
520 $aA 6-year field monitoring study was designed to compare the presence of fall armyworm Spodoptera frugiperda (J. E. Smith), leaf injury, its parasitoids, 100 seed weight, and seed yield of Bt maize hybrids approved for commercialization in the Brazilian market. Field trials were planted in two municipalities, divided into two periods, from 2011 to 2014 (period I), and due to the approval of different Bt hybrids, from 2015 to 2016 (period II). Treatments were Bt, non-Bt maize and all non-Bt maize sprayed with insecticide methomyl. Six Bt-maize hybrids were planted, expressing the following proteins: Cry1Ab, Cry1F, Cry1A.105 (Cry1Ab +Cry1Ac +Cry1F), Cry2Ab2a and Vip3. In 2015 and 2016 Bt Powercore was planted due to its commercial approval. The number of S. frugiperda larvae for most Bt hybrids was lower than on non-Bt hybrids, except for Fórmula TL and Herculex in period II, in both municipalities. In period I, Bt hybrids VT, VT 2 and Viptera showed almost no injury on their leaves, despite the high number of fall armyworm larvae found in plants. Different parasitoids emerged from larvae from Bt and non Bt hybrids: Archytas sp., Campoletis sp., Chelonus sp., Eiphosoma sp. and Ophion luteus. The average 100 seed weight was higher in municipality from Sete Lagoas for all Bt hybrids, non-Bt hybrids and non-Bt hybrids sprayed with methomyl, when compared to Nova Porteirinha during period I. Fórmula TL, non-Bt Fórmula TL sprayed with methomyl, and non-Bt Fórmula TL showed the lowest seed yield in both municipalities and periods.
650 $aAlimento Transgênico
650 $aBacillus Thuringiensis
650 $aMilho
650 $aToxina
653 $aBt
653 $aLagarta-do-cartucho
700 1 $aDANTAS, C. L.
700 1 $aRESENDE, J. P. V.
700 1 $aPAIVA, P. M. de
700 1 $aSOUZA, C. F. de
700 1 $aNASCIMENTO, P. T.
700 1 $aOLIVEIRA, C. R. de
700 1 $aBOREGAS, K. G. B.
700 1 $aRODRIGUEZ-DIMATÉ, F. A.
700 1 $aAGUIAR, R. M.
773 $tRevista Brasileira de Entomologia$gv. 66, n. 2, e20210121, 2022.
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Embrapa Milho e Sorgo (CNPMS) |
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