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| Registros recuperados : 72 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
03/05/2004 |
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Data da última atualização: |
03/05/2004 |
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Autoria: |
RODRIGUES-OTUBO, B. M.; PENTEADO, M. I. de O.; VALLE, C. B. do. |
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Afiliação: |
EMPAER, (Campo Grande, MS). |
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Título: |
Embryo rescue of interspecific hybrids of Brachiaria spp. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, Hague, v. 61, n. 3, p. 175-182, Jan. 2000. |
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Idioma: |
Inglês |
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Notas: |
CNPGC. |
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Conteúdo: |
Age of explant and six different media were evaluated with the objective of regenerating higher numbers of interspecific hybrids between sexual and apomictic Brachiaria. Immature embryos of 7?8, 9?10 and 11?12 days after pollination (DAP), from artificial hybridization between Brachiaria ruziziensis (R) as female parent, and B. brizantha (B) or B. decumbens (D) as male parent, were cultured in modified MS media (M4) ? supplemented with different combinations of growth regulators and vitamins. Embryos cultured 9?12 DAP showed high percentage (85?100%) of germination for all the crosses examined. Germination and survival rates varied according to accessions within crosses. Six different media (all modified MS with different growth regulators and vitamins) were tested with the objective of inducing multiple shoots from 7 to 10 DAP embryos, from crosses between R × B. The media M1, supplemented with Kinetin (13.94 µM) and NAA (5.37 µM), and media M3, supplemented with BA (4.44 µM and IAA 2.85 µM), regenerated adventitious shoots and calli about 30?40 days after inoculation. The highest multiplication rate observed was 2.85 shoots per explant in media M1, 60?70 days after culturing. Two other media, M6, supplemented with 2,4-D (13.57 µM) and M2, with 2,4-D (9.05 µM) and BA (8.87 µM) exclusively induced the formation of calli. The described protocols proved to be efficient in regenerating healthy seedlings from immature embryos of interspecific hybrids in Brachiaria.
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Palavras-Chave: |
Embryo; Regeneration. |
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Thesagro: |
Biotecnologia; Brachiaria; Cultura de Tecido; Embrião; Hibridação; Melhoramento Genético Vegetal; Regeneração. |
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Thesaurus NAL: |
biotechnology; cell culture; interspecific hybridization; plant breeding. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02364naa a2200313 a 4500
001 1325512
005 2004-05-03
008 2000 bl --- 0-- u #d
100 1 $aRODRIGUES-OTUBO, B. M.
245 $aEmbryo rescue of interspecific hybrids of Brachiaria spp.
260 $c2000
500 $aCNPGC.
520 $aAge of explant and six different media were evaluated with the objective of regenerating higher numbers of interspecific hybrids between sexual and apomictic Brachiaria. Immature embryos of 7?8, 9?10 and 11?12 days after pollination (DAP), from artificial hybridization between Brachiaria ruziziensis (R) as female parent, and B. brizantha (B) or B. decumbens (D) as male parent, were cultured in modified MS media (M4) ? supplemented with different combinations of growth regulators and vitamins. Embryos cultured 9?12 DAP showed high percentage (85?100%) of germination for all the crosses examined. Germination and survival rates varied according to accessions within crosses. Six different media (all modified MS with different growth regulators and vitamins) were tested with the objective of inducing multiple shoots from 7 to 10 DAP embryos, from crosses between R × B. The media M1, supplemented with Kinetin (13.94 µM) and NAA (5.37 µM), and media M3, supplemented with BA (4.44 µM and IAA 2.85 µM), regenerated adventitious shoots and calli about 30?40 days after inoculation. The highest multiplication rate observed was 2.85 shoots per explant in media M1, 60?70 days after culturing. Two other media, M6, supplemented with 2,4-D (13.57 µM) and M2, with 2,4-D (9.05 µM) and BA (8.87 µM) exclusively induced the formation of calli. The described protocols proved to be efficient in regenerating healthy seedlings from immature embryos of interspecific hybrids in Brachiaria.
650 $abiotechnology
650 $acell culture
650 $ainterspecific hybridization
650 $aplant breeding
650 $aBiotecnologia
650 $aBrachiaria
650 $aCultura de Tecido
650 $aEmbrião
650 $aHibridação
650 $aMelhoramento Genético Vegetal
650 $aRegeneração
653 $aEmbryo
653 $aRegeneration
700 1 $aPENTEADO, M. I. de O.
700 1 $aVALLE, C. B. do
773 $tPlant Cell, Tissue and Organ Culture, Hague$gv. 61, n. 3, p. 175-182, Jan. 2000.
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