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Registros recuperados : 19 | |
14. | | ARAGÃO, A. P.; GRAÇA, F. A. S.; BALESDENT, A. P.; RODRIGUES, R. L.; ROUSSO FILHO, R. Avaliação da técnica de biópsia hepática por ultra-sonografia em ovinos (Ovis aries) utilizando-se agulha tipo Tru-Cut. Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 30, n. 3, p. 179-182, jul./set. 2008. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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16. | | LAMADE, E.; SETIYO, E.; RODRIGUES, R. L.; FRESNEAU, C.; MAUVE, C.; SKETRIENÉ, D.; TCHERKEZ, G.; GHASHGHAIE, J. Identification of metabolites involved in leaf and bunch edification using 12C/13C on oil palm trees in North Sumatra. In: JOINT EUROPEAN STABLE ISOTOPE USERS GROUP MEETING, 2012, Leipzig, Germany. Conference programme & book of abstracts. Leipzig: Helmholtz Centre for Environmental Research, 2012. p. 152. Biblioteca(s): Embrapa Amazônia Ocidental. |
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17. | | LAMADE, E.; TCHERKEZ, G.; DARLAN, N. H.; RODRIGUES, R. L.; FRESNEAU, C.; MAUVE, C.; LAMOTHE-SIBOLD, M.; SKETRIENÉ, D.; GHASHGHAIE, J. Natural 13C distribution in oil palm (Elaeis guineensis Jacq.) and consequences for allocation pattern. Plant, Cell and Environment, Oxford, v. 39, p. 199-212, 2016. Biblioteca(s): Embrapa Amazônia Ocidental. |
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19. | | SIMÕES-ARAÚJO-ARAUJO, J. L.; RODRIGUES, R. L.; GERHARDT, L. B. de A.; MONDEGO, J. M. C.; ALVES-FERREIRA, M.; RUMJANEK, N. G.; MARGIS-PINHEIRO, M. Identification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. FEBS Letters, Netherlands, v. 515, p. 44-50, 2002. Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 19 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
22/11/2001 |
Data da última atualização: |
31/05/2007 |
Autoria: |
SIMÕES-ARAÚJO-ARAUJO, J. L.; RODRIGUES, R. L.; GERHARDT, L. B. de A.; MONDEGO, J. M. C.; ALVES-FERREIRA, M.; RUMJANEK, N. G.; MARGIS-PINHEIRO, M. |
Título: |
Identification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
FEBS Letters, Netherlands, v. 515, p. 44-50, 2002. |
Idioma: |
Inglês |
Conteúdo: |
Legume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved.
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Palavras-Chave: |
Biological nitrogen fixation; BNF; Caupi; FBN; Fixação bioloógica de nitrogênio. |
Thesagro: |
Vigna Unguiculata. |
Thesaurus NAL: |
cowpeas. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02152naa a2200277 a 4500 001 1598731 005 2007-05-31 008 2002 bl --- 0-- u #d 100 1 $aSIMÕES-ARAÚJO-ARAUJO, J. L. 245 $aIdentification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. 260 $c2002 520 $aLegume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved. 650 $acowpeas 650 $aVigna Unguiculata 653 $aBiological nitrogen fixation 653 $aBNF 653 $aCaupi 653 $aFBN 653 $aFixação bioloógica de nitrogênio 700 1 $aRODRIGUES, R. L. 700 1 $aGERHARDT, L. B. de A. 700 1 $aMONDEGO, J. M. C. 700 1 $aALVES-FERREIRA, M. 700 1 $aRUMJANEK, N. G. 700 1 $aMARGIS-PINHEIRO, M. 773 $tFEBS Letters, Netherlands$gv. 515, p. 44-50, 2002.
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