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Registros recuperados : 126 | |
26. | | RODRIGUES, M. T.; SOARES, M. A. M.; MARQUES, D. S.; SOUZA, A. C. de; NAMBA, V. Estudo do polimorfismo na região do exon 4 do gene da alphaS1-caseina caprina. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., 2007, Jaboticabal. O avanço científico e tecnológico na produção animal: anais. Jaboticabal: Sociedade Brasileira de Zootecnia: UNESP, Faculdade de Ciências Agrárias e Veterinárias, 2007. 3 f. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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28. | | REZENDE, K. T.; SILVA, J. F. C. da; RODRIGUES, M. T.; VALADARES FILHO, S.; SUGOHARA, A. Exigencia de microelementos inorganicos de caprinos em crescimento. II. Magnésio, sódio e potássio. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 27., 1990, Campinas. Anais... Piracicaba: SBZ, 1990. p. 73. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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32. | | SILVA, S. P. da; MATOS, R. da S.; BRITO, L. F.; SILVA, M. M. C. da; RODRIGUES, M. T. Acidez titulável e lipólise no leite caprino em função do tempo de armazenamento no tanque de refrigeração. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras. Biotecnologia e sustentabilidade: anais. Brasília, DF: SBZ; Lavras: UFLA, 2008. 1 CD-ROM. Palavras?chave: características físico-químicas, Dornic, qualidade Biblioteca(s): Embrapa Caprinos e Ovinos. |
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34. | | REZENDE, K. T.; SILVA, J. F. C. da; RODRIGUES, M. T.; GARCIA, J. A.; SILVA, D. J.; CAMPOS, J. Avaliação da gravidade específica da carcaça e da seção da 9ª a 11ª costela para estimar a composição corporal de caprinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 26., 1989, Porto Alegre. Anais... Belo Horizonte: Sociedade Brasileira de Zootecnia, 1989. p. 177. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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35. | | ARAÚJO, A. M. de; GUIMARÃES, S. E. F.; PEREIRA C. S.; LOPES, P. S.; RODRIGUES, M. T.; COLUMBIANO, V. S. Paternidade em rebanhos caprinos por meio de microssatélites de DNA. In: SIMPÓSIO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO ANIMAL, 5., 2004, Pirassunuga, SP. Anais... Pirassunuga: Sociedade Brasileira de Melhoramento Animal, 2004. 4 f. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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36. | | GONÇALVES, A. L.; LANA, R. de P.; RODRIGUES, M. T.; VIEIRA, R. A. M.; QUEIROZ, A. C.; HENRIQUE, D. S. Padrão nictemeral do pH ruminal e comportamento alimentar de cabras leiteiras alimentadas com dietas contendo diferentes relações volumoso:concentrado. Revista Brasileira de Zootecnia, Viçosa, MG, v. 30, n. 6, p. 1886-1892, 2001. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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37. | | GONCALVES, A. L.; LANA, R. de P.; RODRIGUES, M. T.; VIEIRA, R. A. M.; QUEIROZ, A. C. de. Comportamento alimentar de cabras leiteiras submetidas a dietas com diferente relação volumoso:concentrado. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 2000. 3 f. 1 CD ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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38. | | GONCALVES, A. L.; LANA, R. de P.; RODRIGUES, M. T.; VIEIRA, R. A. M.; QUEIROZ, A. C. de. Cinetica de degradacao de alguns volumosos usados na alimentacao de cabras leiteiras por intermedio da tecnica de producao de gases sob diferentes niveis de pH. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba, SP. Anais... Piracicaba: FEALQ, 2001. p.1224-1226. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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39. | | FADEL, A. L.; DELLA COLETTA FILHO, H.; RODRIGUES, C. M.; RODRIGUES, M. T. F.; STUCHI, E. S. Reação da cultivar Navelina ISA 315 (Citrus sinensis L. Osb.), à clorose variegada dos citros em condições de campo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. 1 CD-ROM. pdf 128 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 126 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
09/04/2010 |
Data da última atualização: |
25/09/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
FONSECA, J. F. da; TORRES, C. A. A.; MAFFILI, V. V.; BORGES, A. M.; SANTOS, A. D. F.; RODRIGUES, M. T.; OLIVEIRA, R. F. M. |
Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; UFV, Viçosa, MG; UFV; UFV; UFV; UFV; UFV. |
Título: |
The hypoosmotic swelling test in fresh goat spermatozoa. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Animal Reproduction, v. 2, n. 2, p. 139-144, Apr./Jun. 2005. |
Idioma: |
Inglês |
Conteúdo: |
The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa. MenosThe hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to ... Mostrar Tudo |
Palavras-Chave: |
Goat; Hypoosmotic swelling test; Sperm. |
Thesagro: |
Caprino; Congelamento; Reprodução animal; Sêmen. |
Thesaurus NAL: |
Animal reproduction; Tail. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/75517/1/API-The-hypoosmotic.pdf
|
Marc: |
LEADER 02537naa a2200301 a 4500 001 1663713 005 2019-09-25 008 2005 bl uuuu u00u1 u #d 100 1 $aFONSECA, J. F. da 245 $aThe hypoosmotic swelling test in fresh goat spermatozoa.$h[electronic resource] 260 $c2005 520 $aThe hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa. 650 $aAnimal reproduction 650 $aTail 650 $aCaprino 650 $aCongelamento 650 $aReprodução animal 650 $aSêmen 653 $aGoat 653 $aHypoosmotic swelling test 653 $aSperm 700 1 $aTORRES, C. A. A. 700 1 $aMAFFILI, V. V. 700 1 $aBORGES, A. M. 700 1 $aSANTOS, A. D. F. 700 1 $aRODRIGUES, M. T. 700 1 $aOLIVEIRA, R. F. M. 773 $tAnimal Reproduction$gv. 2, n. 2, p. 139-144, Apr./Jun. 2005.
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