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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/06/2017 |
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Data da última atualização: |
16/06/2017 |
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Autoria: |
TSONIS, C. G.; McNEILLY, A. S.; BAIRD, D. T. |
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Título: |
Measurement of exogenous and endogenous inhibin in sheep serum using a new and extremely sensitive bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Journal of Endocrinology, v. 110, n. 2, p. 341-352, Aug. 1986. |
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DOI: |
10.1677/joe.0.1100341 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ovarian vein blood of sheep with the possibility of extending this to man. MenosAbstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ... Mostrar Tudo |
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Palavras-Chave: |
Biological Assay; Blood supply; Drug effects; FSH; Half-Life; Inhibins; Ovaries. |
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Thesaurus Nal: |
Pharmacology; pituitary gland; Rats; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02435naa a2200289 a 4500
001 2070989
005 2017-06-16
008 1986 bl uuuu u00u1 u #d
024 7 $a10.1677/joe.0.1100341$2DOI
100 1 $aTSONIS, C. G.
245 $aMeasurement of exogenous and endogenous inhibin in sheep serum using a new and extremely sensitive bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro.
260 $c1986
520 $aAbstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ovarian vein blood of sheep with the possibility of extending this to man.
650 $aPharmacology
650 $apituitary gland
650 $aRats
650 $aSheep
653 $aBiological Assay
653 $aBlood supply
653 $aDrug effects
653 $aFSH
653 $aHalf-Life
653 $aInhibins
653 $aOvaries
700 1 $aMcNEILLY, A. S.
700 1 $aBAIRD, D. T.
773 $tJournal of Endocrinology$gv. 110, n. 2, p. 341-352, Aug. 1986.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
25/11/2002 |
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Data da última atualização: |
25/11/2002 |
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Autoria: |
TORQUILHO, D. F.; GODOY, R. L. O.; CASTRO, I. M.; CRASTO, R. V. P.; RODRIGUES, M. G. V.; TORQUILHO, H. S.; QUINTERO, L. M. C. |
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Título: |
Avaliacao de residuos de carbofuran em banana prata ana. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
In: SIMPOSIO LATINO AMERICANO DE CIENCIA DE ALIMENTOS, 4., 2001, Campinas, SP. Livro de Resumos... 2001. p.363, ref. 1249-864. |
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Idioma: |
Português |
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Palavras-Chave: |
Banana prata ana; Pos-coleleita. |
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Thesagro: |
Resíduo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00679naa a2200217 a 4500
001 1414215
005 2002-11-25
008 2001 bl --- 0-- u #d
100 1 $aTORQUILHO, D. F.
245 $aAvaliacao de residuos de carbofuran em banana prata ana.
260 $c2001
650 $aResíduo
653 $aBanana prata ana
653 $aPos-coleleita
700 1 $aGODOY, R. L. O.
700 1 $aCASTRO, I. M.
700 1 $aCRASTO, R. V. P.
700 1 $aRODRIGUES, M. G. V.
700 1 $aTORQUILHO, H. S.
700 1 $aQUINTERO, L. M. C.
773 $tIn: SIMPOSIO LATINO AMERICANO DE CIENCIA DE ALIMENTOS, 4., 2001, Campinas, SP. Livro de Resumos... 2001. p.363, ref. 1249-864.
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Embrapa Agroindústria de Alimentos (CTAA) |
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