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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
22/09/2017 |
Data da última atualização: |
30/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUZA, R. A. V. de; ALVES, M. de C.; CARNEIRO, N. P.; BARROS, B. de A.; BOREM, A.; CARNEIRO, A. A. |
Afiliação: |
Rafaeli Aparecida Vieira de Souza, Universidade Federal de Viçosa; MEIRE DE CASSIA ALVES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; Aluízio Borém, Universidade Federal de Viçosa; ANDREA ALMEIDA CARNEIRO, CNPMS. |
Título: |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017. |
DOI: |
10.1590/1984-70332017v17n2a20 |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. |
Palavras-Chave: |
Concentração de sal; Transformação genética. |
Thesagro: |
Bactéria; Embriogênese; Genética. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164228/1/Agrobacterium-mediated-1.pdf
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Marc: |
LEADER 01738naa a2200253 a 4500 001 2076138 005 2018-01-30 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1590/1984-70332017v17n2a20$2DOI 100 1 $aSOUZA, R. A. V. de 245 $aAgrobacterium-mediated genetic transformation of a tropical elite maize line.$h[electronic resource] 260 $c2017 520 $aABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. 650 $aBactéria 650 $aEmbriogênese 650 $aGenética 653 $aConcentração de sal 653 $aTransformação genética 700 1 $aALVES, M. de C. 700 1 $aCARNEIRO, N. P. 700 1 $aBARROS, B. de A. 700 1 $aBOREM, A. 700 1 $aCARNEIRO, A. A. 773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 17, p. 133-140, 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
17/08/2011 |
Data da última atualização: |
24/01/2020 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ANTUNES, J. F. G.; RODRIGUES, L. H. A.; OLIVEIRA, S. R. de M. |
Afiliação: |
JOAO FRANCISCO GONCALVES ANTUNES, CNPTIA; L. H. A. RODRIGUES, Unicamp; STANLEY ROBSON DE MEDEIROS OLIVEIRA, CNPTIA. |
Título: |
Data mining for sugarcane crop classification using MODIS data. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: EUROPEAN FEDERATION FOR INFORMATION TECHNOLOGY IN AGRICULTURE, FOOD; THE ENVIRONMENT WORLD CONGRESS ON COMPUTERS IN AGRICULTURE, 8., 2011, Prague. Proceedings... Prague: Czech University of Life Sciences Prague, 2011. |
Páginas: |
p. 55-66. |
ISBN: |
978-80-904830-3-3 |
Idioma: |
Inglês |
Notas: |
EFITA/WCCA 2011. |
Conteúdo: |
MODIS (Moderate Resolution Imaging Spectroradiometer) data provide coverage of large areas and high periodicity. These characteristics are fundamental for monitoring strategic agricultural crops in Brazil, such as sugarcane. Data mining is a promising approach to enhance remote sensing data analysis. The objective of this work was to apply the decision tree induction technique to classify sugarcane crop in São Paulo, Brazil, by using MODIS data. A classification model with a good accuracy has been obtained. Furthermore, such an induction technique allowed for knowledge discovery through decision rules that are relevant for specialists. The results revealed the adherence of data mining techniques to satellite image classification problems. |
Palavras-Chave: |
Árvore de decisão; Cana-de-açúcar em São Paulo; Dados MODIS; Data mining; Decision tree; Mineração de dados; Recuperação da informação. |
Thesagro: |
Sensoriamento Remoto. |
Thesaurus NAL: |
Information retrieval; Remote sensing; Sugarcane. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01813nam a2200301 a 4500 001 1898177 005 2020-01-24 008 2011 bl uuuu u00u1 u #d 020 $a978-80-904830-3-3 100 1 $aANTUNES, J. F. G. 245 $aData mining for sugarcane crop classification using MODIS data.$h[electronic resource] 260 $aIn: EUROPEAN FEDERATION FOR INFORMATION TECHNOLOGY IN AGRICULTURE, FOOD; THE ENVIRONMENT WORLD CONGRESS ON COMPUTERS IN AGRICULTURE, 8., 2011, Prague. Proceedings... Prague: Czech University of Life Sciences Prague$c2011 300 $ap. 55-66. 500 $aEFITA/WCCA 2011. 520 $aMODIS (Moderate Resolution Imaging Spectroradiometer) data provide coverage of large areas and high periodicity. These characteristics are fundamental for monitoring strategic agricultural crops in Brazil, such as sugarcane. Data mining is a promising approach to enhance remote sensing data analysis. The objective of this work was to apply the decision tree induction technique to classify sugarcane crop in São Paulo, Brazil, by using MODIS data. A classification model with a good accuracy has been obtained. Furthermore, such an induction technique allowed for knowledge discovery through decision rules that are relevant for specialists. The results revealed the adherence of data mining techniques to satellite image classification problems. 650 $aInformation retrieval 650 $aRemote sensing 650 $aSugarcane 650 $aSensoriamento Remoto 653 $aÁrvore de decisão 653 $aCana-de-açúcar em São Paulo 653 $aDados MODIS 653 $aData mining 653 $aDecision tree 653 $aMineração de dados 653 $aRecuperação da informação 700 1 $aRODRIGUES, L. H. A. 700 1 $aOLIVEIRA, S. R. de M.
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