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| Registros recuperados : 51 | |
| 23. |  | RODRIGUES, J. C. M.; O'REILLY, D. R.; RIBEIRO, B. M.; SIHLER, W.; SOUZA, M. L. Identification of ecdysteroid UDP- Glucosyl transferase activity in Anticarsia gemmatalis nuclear polyhedrosis virus. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 24., 1995, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1995. p.86. Resumo| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | CASTRO, M. E. B. de; SOUZA, M. L. de; SIHLER, W.; RODRIGUES, J. C. M.; RIBEIRO, B. M. Biologia molecular de baculovírus e seu uso no controle biológico de pragas no Brasil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 10, p. 1733-61, out 1999 Título em inglês: Molecular biology of baculovirus and its use in biological control in Brazil.| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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| 26. | | PIRES, M. V. V.; CABRAL, G. B.; LACERDA, A. L.; RODRIGUES, J. C. M.; CARNEIRO, V. T. C. Expressão do gene gus em diferentes explantes de brachiaria brizantha cv. marandu. ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 41| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 27. | | LACERDA, A. L. M.; CABRAL, G. B.; RODRIGUES, J. C. M.; ARAÚJO, A. C. G.; CARNEIRO, V. T. C. Efeito do período de reativação celular na expressão do gene gus em embriões de Brachiaria brizantha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 44.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | RODRIGUES, J. C. M.; GUIMARÃES, L. A.; BIOLCHI, A.; GALANTE, P.; KOLTUNOW, A. M. G.; CARNEIRO, V. T. de C. Differential expression ot two homologues of the Polycomb group genes Fertilization Independent Endosperm (FIE) and Enhancer of Zeste (EzSET) in male reproductive tissues of apomictic Brachiaria brizantha. In: INTERNATIONAL CONGRESS ON SEXUAL PLANT REPRODUCTION, 21., 2010, Bristol. Programme and abstract book... Bristol: University of Bristol, 2010. p. 69.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | SOUZA, F. X. de; MAIA, A. de H. N.; MARQUES, G. V.; COSTA, J. T. A; COELHO, E. L.; RODRIGUES, J. C. M. Comportamento vegetativo de clones de cajazeira com sete anos de cultivo na Chapada do Apodi, Ceará. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 4p. CD-ROM.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 31. | | DUSI, D. M. de A.; LACERDA, A. L. M.; RODRIGUES, J. C. M.; CABRAL, G. B.; ALMEIDA, J. D. de; CARNEIRO, V. T. de C. Localization of BrizREC-GFP fusion protein in monocot cells. In: INTERNATIONAL SYMPOSIUM ON THE MOLECULAR BREEDING OF FORAGE AND TURF: MBFT, 2010, 6., 2010, Buenos Aires. (Resumos...) Buenos Aires: Ediciones INTA, 2010. P-124.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 33. | | ANJOS, R. M. dos; RODRIGUES, J. C. M.; SOUZA, J. C. de; LABATE, M. T. V.; ARAGÃO, F. J. L.; CERVONE, F.; LABATE, C. A.; VIANNA, G. R. Expressão do gene quimérico PG-PGIP, em soja, visando maior tolerância ao fungo Sclerotinia sclerotiorum. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF, 2023. Anais 2023. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. P. 531| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | LACERDA, A. L. M.; DUSI, D. M. de A.; ALVES, E. R.; RODRIGUES, J. C. M.; GOMES, A. C. M. M.; CARNEIRO, V. T. de C. Expression analyses of Brachiaria brizantha genes encoding ribosomal proteins BbrizRPS8, BbrizRPS15a, and BbrizRPL41 during development of ovaries and anthers. Protoplasma, v. 250, v. 2, p. 505-514, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 35. | | OKADA, T.; HU, Y.; TUCKER, M. R.; TAYLOR, J. M.; JOHNSON, S. D.; SPRIGGS, A.; TSUCHIYA, T.; OELKERS, K.; RODRIGUES, J. C. M.; KOLTUNOW, A. M. G. Enlarging cells initiating apomixis in Hieracium praealtum transition to an embryo sac program prior to entering mitosis. Plant Physiology, v. 163, n. 1, p. 216-231, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 36. | | LEITE, J. de A.; SILVEIRA, E. D.; CABRAL, G. B.; RODRIGUES, J. C. M.; DE SÁ, R. R.; CARNEIRO, V. T. C. Efeito da manose na embriogênese somática de Brachiaria brizantha. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 31.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 37. | | LACERDA, A. L. M.; RODRIGUES, J. C. M.; DUSI, D. M. de A.; ALVES, E. R.; CARNEIRO, V. T. de C. Caracterização da expressão de genes durante o desenvolvimento do ovário em Brachiaria brizantha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 38. | | GUIMARÃES, L. A.; DUSI, D. M. de A.; MASIERO, S.; RESENTINI, F.; GOMES, A. C. M. M.; SILVEIRA, E. D.; FLORENTINO, L. H.; RODRIGUES, J. C. M.; COLOMBO, L.; CARNEIRO, V. T. de C. BbrizAGL6 is differentially expressed during embryo sac formation of apomictic and sexual Brachiaria brizantha plants. Plant Molecular Biology Reporter, v. 31, p. 1397-1406, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 39. | | DUSI, D. M. de A.; ALVES, E. R.; CABRAL, G. B.; MELLO, L. V.; RIGDEN, D. J.; SILVEIRA, E. D.; ALVES‑FERREIRA, M.; GUIMARÃES, L. A.; GOMES, A. C. M. M.; RODRIGUES, J. C. M.; CARNEIRO, V. T. de C. An exonuclease V homologue is expressed predominantly during early megasporogenesis in apomictic Brachiaria brizantha. Planta, v. 258, 2023. Article number: 5. Na publicação: Diva M. A. Dusi, Vera T. C. Carneiro.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 40. | | CARNEIRO, V. T. de C.; ARAUJO, A. C. G. de; DUSI, D. M. de A.; CABRAL, G. B.; RODRIGUES, J. C. M.; ALVES, E. R.; SILVEIRA, E. D.; LACERDA, A. L. M.; GOMES, A. C.; FALCÃO, R. Contribuição da biotecnologia ao domÍnio da apomixia de Brachiaria sp. Brasilia: Embrapa Recursos Genéticos e Biotecnologia, 2003. 4 p. (Embrapa Recursos Genéticos e Biotecnologia. Comunicado Técnico, 95).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/03/2009 |
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Data da última atualização: |
20/12/2023 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
RODRIGUES, J. C. M. |
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Afiliação: |
Julio Carlyle Macedo Rodrigues, Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Epigenetic regulation and inheritance of autonomous seed development in apomictic. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
2007. |
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Páginas: |
217 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (Doutorado em Filosofia) - University of Adelaide. Orientadora: Anna M. G. Koltunow. Co-orientadora: Vera Tavares de Campos Carneiro, Embrapa Recursos Genéticos e Biotecnologia. |
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Conteúdo: |
Apomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only form seeds if pollinated. Autonomous seed initiation is actively repressed in the sexual model plant Arabidopsis by the action of a chromatin remodelling complex encoded by the FERTILIZATION INDEPENDENT SEED (FIS)-class genes. These genes are homologues of the Drosophila PcG complex that also repress gene expression throughout Drosophila development. Mutations in the FIS-class genes lead to elements of apomixis, sueh as autonomous endosperm, and in one particular mutant, autonomous egg cell development. Given the similarity in apomictic and FIS-class gene mutant phenotype we isolated three homologues from sexual and apomictic Hieracium plants: FERTILIZATION INDEPENDENT ENDOSPERM (FIE), MULTICOPY SUPPRESSOR OF IRA1 (MS11) AND RETINOBLASTOMA (RBR). FIS-class genes from sexual and apomicit Hieracium and examined their expression, interaction and function during seed initiation. The isolated Hieracium FIS-class genes were highly conserved in sexual and apomictic plants in terns of gene sequence and temporal and spatial expression pattern. Analysis of protein interactions by yeast-two hybrid showed that the HFIE gene from sexual and apomictic plants does not interact with other complex members in the same manner found in Arabidopsis. Protein modelling uncovered structural differences between the Arabidopsis and Hieracium FIE proteins. RNAi- mediated down-regulation of HFIE in sexual Hieracium did not lead to autonomous seed initiation indicating HFIE was not part of a repressive complexo Down-regulation of HFIE in sexual and apomictic plants revealed the gene was essential for embryo growth and viability. Therefore, FIS-complex genes interact differently in Arabidopsis and Hieracium and have different developmental roles. In summary, the results presented here suggest that the FIS-genes are not mutated in apomictic Hieracium plants, but they interact differently relative to the Arabidopsis counterparts and play a fundamental role in embryogenesis. Thus, engineering autonomous seed into crops will not depend on mutating these genes but rather in uncovering the molecular signal that triggers apomictic development. MenosApomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only... Mostrar Tudo |
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Thesagro: |
Apomixia; Reprodução. |
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Thesaurus NAL: |
Hieracium. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04273nam a2200169 a 4500
001 1167799
005 2023-12-20
008 2007 bl uuuu m 00u1 u #d
100 1 $aRODRIGUES, J. C. M.
245 $aEpigenetic regulation and inheritance of autonomous seed development in apomictic.$h[electronic resource]
260 $a2007.$c2007
300 $a217 p.
500 $aTese (Doutorado em Filosofia) - University of Adelaide. Orientadora: Anna M. G. Koltunow. Co-orientadora: Vera Tavares de Campos Carneiro, Embrapa Recursos Genéticos e Biotecnologia.
520 $aApomixis is an intriguing and agronomically valuable asexual reproductive pathway resulting in seeds that give rise to plants that are identical in genotype to the female parent. Apomixis is absent in agriculturally important seed crops and our work has focused on the analysis of apomixis in the daisy-like genus Hieracium which contains sexual and apomictic species. Prior studies have shown that apomixis in Hieracium is controlled by two dominant loci. The LOA controls the avoidance of meiosis during female gametophyte (embryo sac) formation and the LOP locus is required for fertilization independent embryo formation during seed initiation. The genes conferring apomixis are unknown. In this study we focused on the events of autonomous seed initiation. Cytological examination of apomictic mutants that have lost LOA or LOP and analysis of their progeny enabled us to characterize developmental aspects associated with the function of these loci. Upon removal of LOA meiosis occurs normally and LOP segregates with a 1:1 ratio in the progeny, characterizing maternal gametophytic control. We also show that autonomous embryo formation segregates with autonomous endosperm formation, suggesting that these two loci are closely linked. However, upon meiotic division, embryo lethal components arise and embryo development in apomeiosis mutants was generally defective and seed set was low. Similarly, upon removal of LOP, apomixis initiation occurs normally and unreduced embryo sacs can only form seeds if pollinated. Autonomous seed initiation is actively repressed in the sexual model plant Arabidopsis by the action of a chromatin remodelling complex encoded by the FERTILIZATION INDEPENDENT SEED (FIS)-class genes. These genes are homologues of the Drosophila PcG complex that also repress gene expression throughout Drosophila development. Mutations in the FIS-class genes lead to elements of apomixis, sueh as autonomous endosperm, and in one particular mutant, autonomous egg cell development. Given the similarity in apomictic and FIS-class gene mutant phenotype we isolated three homologues from sexual and apomictic Hieracium plants: FERTILIZATION INDEPENDENT ENDOSPERM (FIE), MULTICOPY SUPPRESSOR OF IRA1 (MS11) AND RETINOBLASTOMA (RBR). FIS-class genes from sexual and apomicit Hieracium and examined their expression, interaction and function during seed initiation. The isolated Hieracium FIS-class genes were highly conserved in sexual and apomictic plants in terns of gene sequence and temporal and spatial expression pattern. Analysis of protein interactions by yeast-two hybrid showed that the HFIE gene from sexual and apomictic plants does not interact with other complex members in the same manner found in Arabidopsis. Protein modelling uncovered structural differences between the Arabidopsis and Hieracium FIE proteins. RNAi- mediated down-regulation of HFIE in sexual Hieracium did not lead to autonomous seed initiation indicating HFIE was not part of a repressive complexo Down-regulation of HFIE in sexual and apomictic plants revealed the gene was essential for embryo growth and viability. Therefore, FIS-complex genes interact differently in Arabidopsis and Hieracium and have different developmental roles. In summary, the results presented here suggest that the FIS-genes are not mutated in apomictic Hieracium plants, but they interact differently relative to the Arabidopsis counterparts and play a fundamental role in embryogenesis. Thus, engineering autonomous seed into crops will not depend on mutating these genes but rather in uncovering the molecular signal that triggers apomictic development.
650 $aHieracium
650 $aApomixia
650 $aReprodução
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