| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
17/02/2021 |
|
Data da última atualização: |
08/12/2021 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
SOUZA, W. R. de; OLIVEIRA, N. G. de; VINECKY, F.; RIBEIRO, A. P.; BASSO, M. F.; CASARI, R. A. das C. N.; DIAS, B. B. A.; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; MOLINARI, H. B. C. |
|
Afiliação: |
Wagner Rodrigo de Souza; Nelson Geraldo de Oliveira; Felipe Vinecky; Ana Paula Ribeiro; Marcos Fernando Basso; Raphael Augusto das Chagas Noqueli Casari; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; ADILSON KENJI KOBAYASHI, CNPAE; Kazuo Nakashima, Japan International Research Center for Agricultural Sciences; Kazuko Yamaguchi-Shinozaki, The University of Tokyo; HUGO BRUNO CORREA MOLINARI, CNPAE. |
|
Título: |
Development of drought-tolerant sugarcane overexpressing the AtDREB2A CA gene. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
In: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. ch. 3-3. |
|
Páginas: |
p. 89-98 |
|
Série: |
(JIRCAS Working Report, 91). |
|
Idioma: |
Inglês |
|
Conteúdo: |
Sugarcane is considered an important economic crop not only for sugar production, but also for ethanol generation, serving as an expandable green alternative to the usage of crude oil. To take advantage of the use of sugarcane as a renewable source for bioethanol production, it is important to increase its productivity without increasing land usage, which includes sugarcane cultivation under hostile conditions, such as waterlimited environments. In this study, we demonstrated that stress-inducible overexpression of the transcription factor AtDREB2A CA conferred drought tolerance in sugarcane subjected to water deficiency under greenhouse conditions. In addition, transgenic plants expressing AtDREB2A CA accumulated higher levels of sucrose than non-transgenic plants. These results indicate that the AtDREB2A CA gene under the control of the stress-inducible promoter ZmRab17 represents a promising strategy for the development of new sugarcane varieties with improved drought tolerance. |
|
Palavras-Chave: |
AtDREB2A CA; ZmRab17 promoter. |
|
Thesaurus Nal: |
Abiotic stress; Saccharum; Transcription factors. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221213/1/jircas-working-report91-89-98.pdf
|
|
Marc: |
LEADER 02123naa a2200337 a 4500
001 2130044
005 2021-12-08
008 2020 bl uuuu u00u1 u #d
100 1 $aSOUZA, W. R. de
245 $aDevelopment of drought-tolerant sugarcane overexpressing the AtDREB2A CA gene.$h[electronic resource]
260 $c2020
300 $ap. 89-98
490 $a(JIRCAS Working Report, 91).
520 $aSugarcane is considered an important economic crop not only for sugar production, but also for ethanol generation, serving as an expandable green alternative to the usage of crude oil. To take advantage of the use of sugarcane as a renewable source for bioethanol production, it is important to increase its productivity without increasing land usage, which includes sugarcane cultivation under hostile conditions, such as waterlimited environments. In this study, we demonstrated that stress-inducible overexpression of the transcription factor AtDREB2A CA conferred drought tolerance in sugarcane subjected to water deficiency under greenhouse conditions. In addition, transgenic plants expressing AtDREB2A CA accumulated higher levels of sucrose than non-transgenic plants. These results indicate that the AtDREB2A CA gene under the control of the stress-inducible promoter ZmRab17 represents a promising strategy for the development of new sugarcane varieties with improved drought tolerance.
650 $aAbiotic stress
650 $aSaccharum
650 $aTranscription factors
653 $aAtDREB2A CA
653 $aZmRab17 promoter
700 1 $aOLIVEIRA, N. G. de
700 1 $aVINECKY, F.
700 1 $aRIBEIRO, A. P.
700 1 $aBASSO, M. F.
700 1 $aCASARI, R. A. das C. N.
700 1 $aDIAS, B. B. A.
700 1 $aNEPOMUCENO, A. L.
700 1 $aKOBAYASHI, A. K.
700 1 $aNAKASHIMA, K.
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aMOLINARI, H. B. C.
773 $tIn: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. ch. 3-3.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
24/05/2022 |
|
Data da última atualização: |
23/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
RIBEIRO, T. S.; SCRAMIN, J. A.; RODRIGUES, J. A. S.; BERNARDES FILHO, R.; COLNAGO, L. A.; FORATO, L. A. |
|
Afiliação: |
RUBENS BERNARDES FILHO, CNPDIA; LUIZ ALBERTO COLNAGO, CNPDIA; LUCIMARA APARECIDA FORATO, CNPDIA. |
|
Título: |
13C ss-NMR Singular value decomposition and fitting for sorghum proteins conformation elucidation. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Polímeros, v. 32, n. 1, e2022009, 2022. |
|
Páginas: |
6 p. |
|
ISSN: |
1678-5169 |
|
DOI: |
10.1590/0104-1428.20210082 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Kafirins, water-insoluble proteins from Sweet Sorghum BR501 grains, have been an alternative to prepare edible coatings for food due to their hydrophobic character. In this work, the secondary structures (SS) content of reduced (SSr) and unreduced (SSu) kafirins were determined by 13C solid-state-NMR spectroscopy using areas of carbonyl peak. The SS elucidate by fitting the signal with the Lorentzian function shows 56% and 59% of α-helix and 40% and 12% of β-sheet structures for SSr and SSu, respectively. The SS also were elucidated by a Singular value decomposition- SVD method shows 55% and 49% of α-helix and 12% and 8% of β-sheet structure for SSr and SSu, respectively. Since SVD does not depend on the operator and has higher correlation coefficients for α-helix (0.96%) and β-sheet (0.91%), it is a reliable method to quantify the SS of insoluble proteins using the 13C NMR signal. |
|
Palavras-Chave: |
13C NMR spectroscopy; Kafirin; Secondary structures proteins. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143402/1/P-3C-ss-NMR-Singular-value-decomposition-and-fitting-for.pdf
|
|
Marc: |
LEADER 01652naa a2200253 a 4500
001 2143402
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
022 $a1678-5169
024 7 $a10.1590/0104-1428.20210082$2DOI
100 1 $aRIBEIRO, T. S.
245 $a13C ss-NMR Singular value decomposition and fitting for sorghum proteins conformation elucidation.$h[electronic resource]
260 $c2022
300 $a6 p.
520 $aKafirins, water-insoluble proteins from Sweet Sorghum BR501 grains, have been an alternative to prepare edible coatings for food due to their hydrophobic character. In this work, the secondary structures (SS) content of reduced (SSr) and unreduced (SSu) kafirins were determined by 13C solid-state-NMR spectroscopy using areas of carbonyl peak. The SS elucidate by fitting the signal with the Lorentzian function shows 56% and 59% of α-helix and 40% and 12% of β-sheet structures for SSr and SSu, respectively. The SS also were elucidated by a Singular value decomposition- SVD method shows 55% and 49% of α-helix and 12% and 8% of β-sheet structure for SSr and SSu, respectively. Since SVD does not depend on the operator and has higher correlation coefficients for α-helix (0.96%) and β-sheet (0.91%), it is a reliable method to quantify the SS of insoluble proteins using the 13C NMR signal.
653 $a13C NMR spectroscopy
653 $aKafirin
653 $aSecondary structures proteins
700 1 $aSCRAMIN, J. A.
700 1 $aRODRIGUES, J. A. S.
700 1 $aBERNARDES FILHO, R.
700 1 $aCOLNAGO, L. A.
700 1 $aFORATO, L. A.
773 $tPolímeros$gv. 32, n. 1, e2022009, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
