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| 162. |  | CAMPOS, S. de C.; Correa, P. C.; Rodrigues, A. P. L.; Nogueira, B. L.; Botelho, F. M.; Oliveira, G. H. H. Perda de água e coeficiente de transpiração de goiabas em relação à superfície-volume. Revista Brasileira de Armazenamento, v. 36, n. 2, p. 195-202, 2011. 36 195-202| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 164. | | MUNOZ, A. E. P.; FLORES, R. M. V.; PEDROZA FILHO, M. X.; BARROSO, R. M.; RODRIGUES, A. P. O.; MATAVELI, M.; REZENDE, F. P. Piscicultores e demais agentes da cadeia produtiva discutem os custos de produção de pirarucu em viveiro escavado em Ariquemes, Rondônia. Palmas: Embrapa Pesca e Aquicultura, 2015. 6 p. (Informativo Campo Futuro, 16).| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 178. | | OLIVEIRA NETO, S. M.; MOREIRA, D. A. L.; GUZZELLA, A. B.; PEREIRA, S. R.; RODRIGUES, A. P. D. C.; LAURA, V. A. Temperaturas constantes para o teste de germinação de sementes de estilosantes. In: SEMINÁRIO INTERNO DE INICIAÇÃO CIENTÍFICA, 4.; ENCONTRO DE PÓS-GRADUAÇÃO STRICTO SENSU, 2., 2009, Campo Grande, MS. [Anais...]. Campo Grande, MS: Ed. da UNIDERP, 2009. 4 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PAES, V. M.; VIEIRA, L. A.; CORREIA, H. H. V.; SA, N. A. R.; MOURA, A. A. A.; SALES, A. D.; RODRIGUES, A. P. R.; MAGALHÃES-PADILHA, D. M.; SANTOS, F. W.; APGAR, G. A.; CAMPELLO, C. C.; CAMARGO, L. S. de A.; FIGUEIREDO, J. R. |
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Afiliação: |
V. M. Paes, LAMOFOPA - CE; L. A. Vieira, LAMOFOPA - CE; H. H. V. Correia, LAMOFOPA - CE; N. A. R. Sa, LAMOFOPA - CE; A. A. A. Moura, Universidade Federal do Ceará; A. D. Sales, LAMOFOPA - CE; A. P. R. Rodrigues, LAMOFOPA - CE; D. M. Magalhães-Padilha, Universidade Potiguar; F. W. Santos, Universidade Estadual do Pampa; G. A. Apgar, Southern Illinois University, Carbondale, Illinois, USA; C. C. Campello, LAMOFOPA - CE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; J. R. Figueiredo, LAMOFOPA - CE. |
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Título: |
Effect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Theriogenology, n. 86, p. 994-1003, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC. MenosAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Oocyte; Preantral follicle. |
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Thesaurus NAL: |
heat stress; in vitro culture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03440naa a2200325 a 4500
001 2062969
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aPAES, V. M.
245 $aEffect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex.$h[electronic resource]
260 $c2016
520 $aAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC.
650 $aheat stress
650 $ain vitro culture
653 $aBovine
653 $aOocyte
653 $aPreantral follicle
700 1 $aVIEIRA, L. A.
700 1 $aCORREIA, H. H. V.
700 1 $aSA, N. A. R.
700 1 $aMOURA, A. A. A.
700 1 $aSALES, A. D.
700 1 $aRODRIGUES, A. P. R.
700 1 $aMAGALHÃES-PADILHA, D. M.
700 1 $aSANTOS, F. W.
700 1 $aAPGAR, G. A.
700 1 $aCAMPELLO, C. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFIGUEIREDO, J. R.
773 $tTheriogenology$gn. 86, p. 994-1003, 2016.
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