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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/07/2022 |
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Data da última atualização: |
20/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. M. de J.; DIAS, R. de C. S.; SANTOS, J. S. dos; SOUZA, F. de F.; MELO, N. F. de. |
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Afiliação: |
CARLA MARIA DE JESUS SILVA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; RITA DE CASSIA SOUZA DIAS, CPATSA; JOICE SIMONE DOS SANTOS, Instituto Federal do Sertão Pernambucano, Campus Ouricuri, PE; FLAVIO DE FRANCA SOUZA, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
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Título: |
Induction of polyploidy in watermelon genotype with powdery mildew resistance (Podosphaera xanthii). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Revista Caatinga, v. 35, n. 3, p. 505-513, 2022. |
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DOI: |
http://dx.doi.org/10.1590/1983-21252022v35n301rc |
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Idioma: |
Inglês |
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Conteúdo: |
Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant. MenosTriploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics... Mostrar Tudo |
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Palavras-Chave: |
Duplicação cromossômica; Melancia triploide; Podosphaera xanthii; Tetraploidia. |
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Thesagro: |
Citogenética; Citogenética Vegetal; Citrullus Lanatus; Doença; Melancia; Oídio. |
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Thesaurus Nal: |
Cytogenetics; Tetraploidy; Watermelons. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144783/1/INDUCTION-OF-POLYPLOIDY-IN-WATERMELON-2022.pdf
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Marc: |
LEADER 02593naa a2200337 a 4500
001 2144783
005 2023-04-20
008 2022 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/1983-21252022v35n301rc$2DOI
100 1 $aSILVA, C. M. de J.
245 $aInduction of polyploidy in watermelon genotype with powdery mildew resistance (Podosphaera xanthii).$h[electronic resource]
260 $c2022
520 $aTriploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.
650 $aCytogenetics
650 $aTetraploidy
650 $aWatermelons
650 $aCitogenética
650 $aCitogenética Vegetal
650 $aCitrullus Lanatus
650 $aDoença
650 $aMelancia
650 $aOídio
653 $aDuplicação cromossômica
653 $aMelancia triploide
653 $aPodosphaera xanthii
653 $aTetraploidia
700 1 $aDIAS, R. de C. S.
700 1 $aSANTOS, J. S. dos
700 1 $aSOUZA, F. de F.
700 1 $aMELO, N. F. de
773 $tRevista Caatinga$gv. 35, n. 3, p. 505-513, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
NASCIMENTO, E. M.; MEDEIROS, R. M. T.; SIMÕES, S. V. D.; RIET-CORREA, F. |
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Afiliação: |
EDUARDO M. NASCIMENTO, HV/FV/UFCG; ROSANE M. T. MEDEIROS, HV/FV/UFCG; SARA V. D. SIMÕES, HV/FV/UFCG; FRANKLIN RIET-CORREA, HV/FV/UFCG/INIA. |
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Título: |
Compactação ruminal e obstrução intestinal em bovinos, associadas ao consumo de Agave sisalana Perrine (Agavaceae). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 719-723, ago. 2016. |
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Idioma: |
Português |
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Conteúdo: |
Indigestion due to compaction of the ruminal content is a digestive disorder resulting from low-quality forage intake, rich in lignin and with low levels of energy and digestible protein, with or without water restriction. The accumulation of plant residues rich in indigestible fibers can also lead to formation of phytobezoars, causing digestive disorders and intestinal obstructions. The objective of this paper was to describe the epidemiology and the clinical and pathological changes of an outbreak of indigestion and other digestive disorders in cattle fed Agave sisalana. The affected herd consisted of 22 adult cattle reared in a semi-extensive system, which ingested almost exclusively stalks of A. sisalana manually cut for two months. Six cows showed decreased appetite and rumination, abdominal distension, and reduced production of dried feces. One animal was referred to the Veterinary Hospital. The presumptive diagnosis of indigestion was made by the epidemiologic data, clinical signs, and laboratory evaluation. At necropsy, the rumen, reticulum and omasum were filled with fibrous and dried A. sisalana content. A viscous, greenish brownish content was present in the abomasum. Numerous phytobezoars were found in the pre-stomachs and abomasum measuring 4-12cm in diameter. One phytobezoar was partially obstructing the duodenum, with the cranial part of the organ filled with intestinal content, and the caudal portion was empty. It is concluded that the administration of A. sisalana stalks for long periods may cause indigestion due to ruminal compaction and formation of phytobezoars. MenosIndigestion due to compaction of the ruminal content is a digestive disorder resulting from low-quality forage intake, rich in lignin and with low levels of energy and digestible protein, with or without water restriction. The accumulation of plant residues rich in indigestible fibers can also lead to formation of phytobezoars, causing digestive disorders and intestinal obstructions. The objective of this paper was to describe the epidemiology and the clinical and pathological changes of an outbreak of indigestion and other digestive disorders in cattle fed Agave sisalana. The affected herd consisted of 22 adult cattle reared in a semi-extensive system, which ingested almost exclusively stalks of A. sisalana manually cut for two months. Six cows showed decreased appetite and rumination, abdominal distension, and reduced production of dried feces. One animal was referred to the Veterinary Hospital. The presumptive diagnosis of indigestion was made by the epidemiologic data, clinical signs, and laboratory evaluation. At necropsy, the rumen, reticulum and omasum were filled with fibrous and dried A. sisalana content. A viscous, greenish brownish content was present in the abomasum. Numerous phytobezoars were found in the pre-stomachs and abomasum measuring 4-12cm in diameter. One phytobezoar was partially obstructing the duodenum, with the cranial part of the organ filled with intestinal content, and the caudal portion was empty. It is concluded that the administration of A. si... Mostrar Tudo |
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Palavras-Chave: |
Fitobezoares; Phytobezoars; Sistema digestório. |
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Thesagro: |
Agave Sisalana; Bovino; Prensagem. |
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Thesaurus NAL: |
Digestive system; Indigestion. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148233/1/Compactacao-ruminal.pdf
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Marc: |
LEADER 02376naa a2200253 a 4500
001 2054017
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, E. M.
245 $aCompactação ruminal e obstrução intestinal em bovinos, associadas ao consumo de Agave sisalana Perrine (Agavaceae).
260 $c2016
520 $aIndigestion due to compaction of the ruminal content is a digestive disorder resulting from low-quality forage intake, rich in lignin and with low levels of energy and digestible protein, with or without water restriction. The accumulation of plant residues rich in indigestible fibers can also lead to formation of phytobezoars, causing digestive disorders and intestinal obstructions. The objective of this paper was to describe the epidemiology and the clinical and pathological changes of an outbreak of indigestion and other digestive disorders in cattle fed Agave sisalana. The affected herd consisted of 22 adult cattle reared in a semi-extensive system, which ingested almost exclusively stalks of A. sisalana manually cut for two months. Six cows showed decreased appetite and rumination, abdominal distension, and reduced production of dried feces. One animal was referred to the Veterinary Hospital. The presumptive diagnosis of indigestion was made by the epidemiologic data, clinical signs, and laboratory evaluation. At necropsy, the rumen, reticulum and omasum were filled with fibrous and dried A. sisalana content. A viscous, greenish brownish content was present in the abomasum. Numerous phytobezoars were found in the pre-stomachs and abomasum measuring 4-12cm in diameter. One phytobezoar was partially obstructing the duodenum, with the cranial part of the organ filled with intestinal content, and the caudal portion was empty. It is concluded that the administration of A. sisalana stalks for long periods may cause indigestion due to ruminal compaction and formation of phytobezoars.
650 $aDigestive system
650 $aIndigestion
650 $aAgave Sisalana
650 $aBovino
650 $aPrensagem
653 $aFitobezoares
653 $aPhytobezoars
653 $aSistema digestório
700 1 $aMEDEIROS, R. M. T.
700 1 $aSIMÕES, S. V. D.
700 1 $aRIET-CORREA, F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 719-723, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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