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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
27/02/2024 |
Data da última atualização: |
12/03/2024 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ARAÚJO, G. L. T.; BASSO, M. F.; MORGANTE, C. V.; SA, M. F. G. de. |
Afiliação: |
GABRIELE LOUISE TRINDADE ARAÚJO, Universidade Católica de Brasília; MARCOS FERNANDO BASSO, Universidade Católica de Brasília; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Overexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. |
Páginas: |
p. 611. |
Idioma: |
Inglês |
Conteúdo: |
Nematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events were generated with a 1% transformation efficiency. Characterization of transgenic plants was performed by PCR and enzyme-linked immunosorbent assays. Five independent transformation events with high transgenic protein accumulation were selected for generation advancement. Progenies of T2 generation were inoculated with 2000 ppJ2 juveniles of M. incognita at 15 days after seed germination in an assay performed in greenhouse with 20 replicates of each event. After 90 days, the roots were evaluated for the incidence of galls using a qualitative rating scale. Two transgenic events stood out by having superior scores, indicating a reduction in the number of galls. Additional analyses are in progress to determine quantitative effects of the parasitism in transgenic plants. Biotechnology approaches offer great potential for application in commercial crop cultivars aiming to reduce plant susceptibility to root-knot nematodes and develop sustainable agriculture with less dependence on pesticides. MenosNematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events wer... Mostrar Tudo |
Palavras-Chave: |
Nematóides das galhas; Plantas geneticamente modificadas. |
Thesagro: |
Algodão; Meloidogyne Incognita; Nematóide; Produção Agrícola. |
Thesaurus Nal: |
Cotton; Genetically modified plants; Root-knot nematodes. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162324/1/Overexpression-of-the-GmGlb1-1-and-GmEXPA-1-applied-in-cotton-to-increase-tolerance-to-Meloidogyne-incognita.pdf
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Marc: |
LEADER 03410nam a2200265 a 4500 001 2162324 005 2024-03-12 008 2023 bl uuuu u00u1 u #d 100 1 $aARAÚJO, G. L. T. 245 $aOverexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia$c2023 300 $ap. 611. 520 $aNematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events were generated with a 1% transformation efficiency. Characterization of transgenic plants was performed by PCR and enzyme-linked immunosorbent assays. Five independent transformation events with high transgenic protein accumulation were selected for generation advancement. Progenies of T2 generation were inoculated with 2000 ppJ2 juveniles of M. incognita at 15 days after seed germination in an assay performed in greenhouse with 20 replicates of each event. After 90 days, the roots were evaluated for the incidence of galls using a qualitative rating scale. Two transgenic events stood out by having superior scores, indicating a reduction in the number of galls. Additional analyses are in progress to determine quantitative effects of the parasitism in transgenic plants. Biotechnology approaches offer great potential for application in commercial crop cultivars aiming to reduce plant susceptibility to root-knot nematodes and develop sustainable agriculture with less dependence on pesticides. 650 $aCotton 650 $aGenetically modified plants 650 $aRoot-knot nematodes 650 $aAlgodão 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aProdução Agrícola 653 $aNematóides das galhas 653 $aPlantas geneticamente modificadas 700 1 $aBASSO, M. F. 700 1 $aMORGANTE, C. V. 700 1 $aSA, M. F. G. de
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Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
22/07/2014 |
Data da última atualização: |
16/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
TONIETTO, J.; SOTES RUIZ, V.; ZANUS, M. C.; MONTES, C.; ULIARTE, E. M.; BRUNO, L. A.; CLIMACO, P.; PENA, A.; GUERRA, C. C.; CATANIA, C. D.; KOHLBERG, E. J.; PEREIRA, G. E.; RICARDO-DA-SILVA, J.-M.; RAGOUT, J. V.; NAVARRO, L. V.; LAUREANO, O.; CASTRO, R. de; DEL MONTE, R. F.; DEL MONTE, S. A.; GOMEZ-MIGUEL, V.; CARBONNEAU, A. |
Afiliação: |
JORGE TONIETTO, CNPUV; UPM - Universidad Politécnica, Madrid, Spain; MAURO CELSO ZANUS, CNPUV; Centro de Estudios Avanzados en Zonas Áridas, La Serena, Chile; INTA – EEA, Mendoza, Argentina; PFCUVS-FAUTAPO, Tarija, Bolivia; INIA/INRB, Estação Vitivinícola Nacional, Dois Portos, Portugal; Faculdad de Ciencias Agronomicas, Universidad de Chile, Santiago, Chile; CELITO CRIVELLARO GUERRA, CNPUV; INTA – EEA, Mendoza, Argentina; Kohlberg Estate, Tarija, Bolivia; GIULIANO ELIAS PEREIRA, CNPUV / CPATSA; ISA-UTL - Instituto Superior de Agronomia, Lisboa, Portugal; Enology expert, Spain; Enology expert, Spain; ISA-UTL - Instituto Superior de Agronomia, Lisboa, Portugal; ISA-UTL - Instituto Superior de Agronomia, Lisboa, Portugal; INTA – EEA, Mendoza, Argentina; INTA – EEA, Mendoza, Argentina; UPM - Universidad Politécnica, Madrid, Spain; Supagro, Montpellier, France. |
Título: |
The Effect of viticultural climate on red and white wine typicity: characterization in Ibero-American grape-growing regions. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Journal International des Sciences de la Vigne et du Vin, Bordeaux, p. 19-23, 2014. |
Idioma: |
Inglês |
Notas: |
Spécial Laccave. |
Conteúdo: |
This study is part of a CYTED (Ibero-American Program for Science, Technology and Development) project on vitivinicultural zoning. The objective was to characterize the effect of viticultural climate on red and white wine typicity in the macro Ibero-American viticultural region. |
Palavras-Chave: |
Mudanças Climáticas; Tipicidade do vinho; Variáveis sensoriais; Vinho branco; Vinho tinto. |
Thesagro: |
Uva; Vinho; Viticultura; Vitis Vinifera. |
Thesaurus NAL: |
Climate change; Grapes; Principal component analysis; Viticulture. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01754naa a2200529 a 4500 001 1991136 005 2017-05-16 008 2014 bl uuuu u00u1 u #d 100 1 $aTONIETTO, J. 245 $aThe Effect of viticultural climate on red and white wine typicity$bcharacterization in Ibero-American grape-growing regions. 260 $c2014 500 $aSpécial Laccave. 520 $aThis study is part of a CYTED (Ibero-American Program for Science, Technology and Development) project on vitivinicultural zoning. The objective was to characterize the effect of viticultural climate on red and white wine typicity in the macro Ibero-American viticultural region. 650 $aClimate change 650 $aGrapes 650 $aPrincipal component analysis 650 $aViticulture 650 $aUva 650 $aVinho 650 $aViticultura 650 $aVitis Vinifera 653 $aMudanças Climáticas 653 $aTipicidade do vinho 653 $aVariáveis sensoriais 653 $aVinho branco 653 $aVinho tinto 700 1 $aSOTES RUIZ, V. 700 1 $aZANUS, M. C. 700 1 $aMONTES, C. 700 1 $aULIARTE, E. M. 700 1 $aBRUNO, L. A. 700 1 $aCLIMACO, P. 700 1 $aPENA, A. 700 1 $aGUERRA, C. C. 700 1 $aCATANIA, C. D. 700 1 $aKOHLBERG, E. J. 700 1 $aPEREIRA, G. E. 700 1 $aRICARDO-DA-SILVA, J.-M. 700 1 $aRAGOUT, J. V. 700 1 $aNAVARRO, L. V. 700 1 $aLAUREANO, O. 700 1 $aCASTRO, R. de 700 1 $aDEL MONTE, R. F. 700 1 $aDEL MONTE, S. A. 700 1 $aGOMEZ-MIGUEL, V. 700 1 $aCARBONNEAU, A. 773 $tJournal International des Sciences de la Vigne et du Vin, Bordeaux, p. 19-23, 2014.
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