Data/hora: 04/06/2024 05:48:31

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 11/12/2013
Data da última atualização: | 18/03/2015
Autoria: | SANTOS, J. M. L. dos; MONTEIRO, J. P.; RIBEIRO, W. L. C.; MACEDO, I. T. F.; CAMURÇA-VASCONCELOS, A. L. F.; VIEIRA, L. da S.; BEVILÁQUA, C. M. L.
Afiliação: | Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; Wesley Lyeverton Correia Ribeiro, Pós-graduação - UECe - Fortaleza, CE, Brazil; Iara Tersia Freitas Macedo, UECe - Fortaleza, CE, Brazil; Ana Lourdes Fernandes Camurça-Vasconcelos, Pós-graduação - UECe - Fortaleza, CE, Brazil; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, Pós-graduação - UECe - Fortaleza, CE, Brazil.
Título: | Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil.
Ano de publicação: | 2014
Fonte/Imprenta: | Veterinary Parasitology, v. 199, n. 3/4, p. 160-164, Jan. 2014.
DOI: | http://dx.doi.org/10.1016/j.vetpar.2013.11.006
Idioma: | Inglês
Conteúdo: | Abstract: Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the ?-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 ug/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.
Palavras-Chave: | Gastrointestinal diseases; Helmintose; Nematóide gastrintestinal; PCR; QPCR; Quantitative real-time PCR; Resistance to anthelmintics; Resistência anti-helmíntica.
Thesagro: | Anti-helmíntico; Haemonchus contortus; Nematóide; Ovino.
Thesaurus Nal: | benzimidazole; Sheep.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (27754-1) - UPC - AP - DD -  -  -  - DIS