Data/hora: 03/06/2024 15:37:08

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 02/02/2017
Data da última atualização: | 19/10/2020
Autoria: | DELAMUTA, J. R. M.; RIBEIRO, R. A.; ARAUJO, J. L. S. de; ROUWS, L. F. M.; ZILLI, J. E.; PARMA, M. M.; MELO, I. S. de; HUNGRIA, M.
Afiliação: | JAKELINE RENATA MARCON DELAMUTA, CAPES; RENAN AUGUSTO RIBEIRO, CNPq; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JERRI EDSON ZILLI, CNPAB; MARCIA MARIA PARMA LEME, CNPMA; ITAMAR SOARES DE MELO, CNPMA; MARIANGELA HUNGRIA DA CUNHA, CNPSO.
Título: | Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.
Ano de publicação: | 2016
Fonte/Imprenta: | International Journal of Systematic and Evolutionary Microbiology, v. 66, n. 8, p. 3078-3087, 2016.
DOI: | http://dx.doi.org/10.1099/ijsem.0.001148
Idioma: | Inglês
Conteúdo: | Abstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4&#8201;% similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5&#8201;% nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7&#8201;%). DNA-DNA hybridization resulted in &#916;Tm values over 6.7&#8201;°C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B.huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis.
Palavras-Chave: | Bradyrhizobium stylosanthis.
Thesagro: | Fixação simbiótica de nitrogênio; Planta forrageira; Rhizobium.
Thesaurus Nal: | Nitrogen-fixing bacteria; Stylosanthes.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (15254-1) - UPC - AP - DD -  -  -  - DIS