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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
19/10/2004 |
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Data da última atualização: |
15/03/2011 |
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Autoria: |
OLIVEIRA, R. P. de; AGUILAR-VILDOSO, C. I.; MACHADO, M. A. |
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Título: |
Determination of the genome size and the number of markers for saturation of linkage maps of citrus. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 3, p. 417-423, Sept. 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to determine the size of the genome of citrus and correlate it with the number of markers necessary to saturate linkage maps. Linkage maps of 'Pêra' sweet orange (Citrus sinensis (L.) Osbeck) and 'Cravo' mandarin (C. reticulata Blanco) were constructed using 123 and 53 RAPD markers, respectively. The estimation of the genome size was conducted using regression models, the method of moments and Poisson probability. These approaches revealed a significant variation when estimating the genomes of 'Pêra' (612 to 1731 cM) and 'Cravo' (392 to 698 cM). The average number of markers necessary to saturate 99% of the genetic length determined for the genomes of 'Pêra' and 'Cravo' was 183 and 98, respectively. The linkage maps of these two varieties showed that the markers were distributed in clusters and this affected the accuracy of the estimates, especially of the 'Cravo' mandarin. |
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Palavras-Chave: |
Citros; Method of moments; Poisson probability; RAPD; Regression method. |
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Thesagro: |
Genoma; Saturação; Tamanho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01631naa a2200241 a 4500
001 1212605
005 2011-03-15
008 2002 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, R. P. de
245 $aDetermination of the genome size and the number of markers for saturation of linkage maps of citrus.
260 $c2002
520 $aThe objective of this study was to determine the size of the genome of citrus and correlate it with the number of markers necessary to saturate linkage maps. Linkage maps of 'Pêra' sweet orange (Citrus sinensis (L.) Osbeck) and 'Cravo' mandarin (C. reticulata Blanco) were constructed using 123 and 53 RAPD markers, respectively. The estimation of the genome size was conducted using regression models, the method of moments and Poisson probability. These approaches revealed a significant variation when estimating the genomes of 'Pêra' (612 to 1731 cM) and 'Cravo' (392 to 698 cM). The average number of markers necessary to saturate 99% of the genetic length determined for the genomes of 'Pêra' and 'Cravo' was 183 and 98, respectively. The linkage maps of these two varieties showed that the markers were distributed in clusters and this affected the accuracy of the estimates, especially of the 'Cravo' mandarin.
650 $aGenoma
650 $aSaturação
650 $aTamanho
653 $aCitros
653 $aMethod of moments
653 $aPoisson probability
653 $aRAPD
653 $aRegression method
700 1 $aAGUILAR-VILDOSO, C. I.
700 1 $aMACHADO, M. A.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 2, n. 3, p. 417-423, Sept. 2002.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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