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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/03/2009 |
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Data da última atualização: |
16/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARCELINO, F. C.; GUIMARÃES, M. F. M.; BARROS, E. G. |
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Afiliação: |
Francismar Correa Marcelino, Embrapa Soja; Marta Fonseca Martins Guimaraes, Embrapa Gado de Leite; Everaldo Gonçalves de Barros, Bioagro / UFV. |
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Título: |
Detection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Ciência e Tecnologia de Alimentos, Campinas, v. 28, p. 38-45, 2008. |
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DOI: |
https://doi.org/10.1590/S0101-20612008000500007 |
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Idioma: |
Inglês |
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Notas: |
Supl. |
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Conteúdo: |
The increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory. MenosThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit vari... Mostrar Tudo |
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Palavras-Chave: |
GMO; PCR quantitative; Sausage; Transgenic residues. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596463/1/Detection-and-quantification-of-Roundup-Ready-soybean.pdf
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Marc: |
LEADER 02405naa a2200217 a 4500
001 1596463
005 2024-02-16
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0101-20612008000500007$2DOI
100 1 $aMARCELINO, F. C.
245 $aDetection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR.$h[electronic resource]
260 $c2008
500 $aSupl.
520 $aThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory.
653 $aGMO
653 $aPCR quantitative
653 $aSausage
653 $aTransgenic residues
700 1 $aGUIMARÃES, M. F. M.
700 1 $aBARROS, E. G.
773 $tCiência e Tecnologia de Alimentos, Campinas$gv. 28, p. 38-45, 2008.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
SILVA, M. S. L. da; RIBEIRO, M. R. |
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Afiliação: |
MARIA SONIA LOPES DA SILVA, CPATSA. |
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Título: |
Influencia do cultivo continuo da cana-de-acucar em propriedades morfologicas e fisicas de solos argilosos de tabuleiro no Estado de Alagoas. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência do Solo, Campinas, v. 16, p. 397-402, 1992. |
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Idioma: |
Português |
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Conteúdo: |
Com o objetivo de avaliar o efeito do cultivo continuo da cana-de-acucar em propriedades de solos de tabuleiro, foram selecionados quatro talhoes da Usina Santa Clotilde, municipio de Rio Largo, AL, sendo um coberto por vegetacao de mata nativa e os demais, cultivados por periodos de 5,15 e 25 anos. Os solos foram caracterizados morfologicamente e nas amostras, coletadas por horizontes, foram determinadas a granulometria, umidade a -1/3 e /15 bar, densidade do solo, estabilidade de agregados, porosidade total, macro- e microporosidade. Os resultados mostraram: (a) que os solos tem uma morfologia similar, exceto para o horizonte superficial, onde a utilizacao agricola promoveo u desenvolvijmento de um horizonte Ap, com transicao abrupta para o horizonte BA subjacente, e (b) que, com relacao as propriedades fisicas, nas condicoes de solo e manejo das areas estudadas, o cultivo continuo da cana-de-acucar nao alterou de forma expressiva as caracteristicas dos solos, podendo ser observadas, apenas, pequenas modificacoes restritas ao horizonte superficial. |
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Palavras-Chave: |
Alagoas; Brasil; Cana-de-açúcar; Morfologia; Morphology; Physic; Plateau; Soils; Solos; Sugar cane; Tabuleiros. |
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Thesagro: |
Cana de Açúcar; Cultivo Continuo; Física; Física do Solo; Solo. |
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Thesaurus NAL: |
soil; sugarcane. |
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Categoria do assunto: |
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P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178460/1/Revista-brasileira-de-ciencia-do-solo-v.16n.3p.397-402.pdf
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Marc: |
LEADER 02010naa a2200349 a 4500
001 1133350
005 2018-06-12
008 1992 bl uuuu u00u1 u #d
100 1 $aSILVA, M. S. L. da
245 $aInfluencia do cultivo continuo da cana-de-acucar em propriedades morfologicas e fisicas de solos argilosos de tabuleiro no Estado de Alagoas.
260 $c1992
520 $aCom o objetivo de avaliar o efeito do cultivo continuo da cana-de-acucar em propriedades de solos de tabuleiro, foram selecionados quatro talhoes da Usina Santa Clotilde, municipio de Rio Largo, AL, sendo um coberto por vegetacao de mata nativa e os demais, cultivados por periodos de 5,15 e 25 anos. Os solos foram caracterizados morfologicamente e nas amostras, coletadas por horizontes, foram determinadas a granulometria, umidade a -1/3 e /15 bar, densidade do solo, estabilidade de agregados, porosidade total, macro- e microporosidade. Os resultados mostraram: (a) que os solos tem uma morfologia similar, exceto para o horizonte superficial, onde a utilizacao agricola promoveo u desenvolvijmento de um horizonte Ap, com transicao abrupta para o horizonte BA subjacente, e (b) que, com relacao as propriedades fisicas, nas condicoes de solo e manejo das areas estudadas, o cultivo continuo da cana-de-acucar nao alterou de forma expressiva as caracteristicas dos solos, podendo ser observadas, apenas, pequenas modificacoes restritas ao horizonte superficial.
650 $asoil
650 $asugarcane
650 $aCana de Açúcar
650 $aCultivo Continuo
650 $aFísica
650 $aFísica do Solo
650 $aSolo
653 $aAlagoas
653 $aBrasil
653 $aCana-de-açúcar
653 $aMorfologia
653 $aMorphology
653 $aPhysic
653 $aPlateau
653 $aSoils
653 $aSolos
653 $aSugar cane
653 $aTabuleiros
700 1 $aRIBEIRO, M. R.
773 $tRevista Brasileira de Ciência do Solo, Campinas$gv. 16, p. 397-402, 1992.
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Embrapa Semiárido (CPATSA) |
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