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Registros recuperados : 31 | |
9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MENNA, P.; HUNGRIA, M.; BARCELLOS, F. G.; BANGEL, E. V.; HESS, P. N.; MARTÍNEZ-ROMERO, E. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. Systematic and Applied Microbiology, Stuttgart, v.29, n. 4, p. 315-332, Jun. 2006. Biblioteca(s): Embrapa Soja. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARTINEZ ROMERO, E.; SEGOVIA, L.; MERCANTE, F. M.; FRANCO, A. A.; GRAHAM, P.; PARDO, M. A. Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int. J. Syst. Bacteriol., v.41, n.3, p.417-426, 1991. Biblioteca(s): Embrapa Agrobiologia. |
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11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, R.; HUNGRIA, M.; ROGEL, M.; LOPEZ-LOPEZ, A.; ORMEÑO-ORRILLO, E.; MARTINEZ-ROMERO, E. Taxonomia e filogenia de microssimbiontes de feijoeiro (Phaseolous vulgaris L.) com o uso da técnica de MLSA (Multilocus Sequence Analysis). In: REUNIÓN LATINOAMERICANA DE RIZOBIOLOGÍA, 25.; CONGRESO NACIONAL DE MICROORGANISMOS PROMOTORES DEL CRECIMIENTO VEGETAL, 1., 2011, Piriápolis. Libro de resúmenes. Programa. Piriápolis: ALAR, 2011. p. 62. Biblioteca(s): Embrapa Soja. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ORMEÑO-ORRILLO, E.; ROGEL, M. A.; CHUEIRE, L. M. O.; TIEDJE, J. M.; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. Genome sequences of Burkholderia sp. Strains CCGE1002 and H160, isolated from legume nodules in Mexico and Brazil. Journal of Bacteriology, Washington, v. 194, n. 24, p. 6927, Dec. 2012. Biblioteca(s): Embrapa Soja. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, R. A.; ORMEÑO-ORILLO, E.; DALL'AGNOL, R. F.; GRAHAM, P. H.; MARTINEZ-ROMERO, E.; HUNGRIA, M. Novel Rhizobium lineages isolated from root nodules of the common bean (Phaseolus vulgaris L.) in Andean and Mesoamerican areas. Research in Microbiology, v. 164, n. 7, p. 740-748, Sept. 2013. Biblioteca(s): Embrapa Soja. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DELAMUTA, J. R. M.; RIBEIRO, R. A.; ORMEÑO-ORRILLO, E.; MELO, I. S.; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal of Systematic Evolutionary Microbiology, Reading, v. 63, n. 9, p. 3342-3351, Mar. 2013. Biblioteca(s): Embrapa Meio Ambiente; Embrapa Soja. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DELAMUTA, J. R. M.; RIBEIRO, R. A.; ORMEÑO-ORRILLO, E.; PARMA, M. M.; MELO, I. S. de; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. Bradyrhizobium tropiciagri sp. nov. and Bradyrhizobium embrapense sp. nov., nitrogen-fixing symbionts of tropical forage legumes. International Journal of Systematic and Evolutionary Microbiology, Reading, v. 65, n. 12, p. 4424-4433, 2015. Biblioteca(s): Embrapa Meio Ambiente; Embrapa Soja. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | HELENE, L. C. F.; DELAMUTA, J. R. M.; RIBEIRO. R. A.; ORMEÑO-ORRILLO, E.; ROGEL, M. A; MARTINEZ-ROMERO, E.; HUNGRIA, M. Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services. International Journal of Systematic and Evolutionary Microbiology, Reading v. 65, n. 12, 2015. p. 4441-4438 Biblioteca(s): Embrapa Soja. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FUENTES-RAMÍREZ, L. E.; BUSTILLOS-CRISTALES, R.; TAPIA-HERNÁNDEZ, A.; JIMÉNEZ-SALGADO, T.; WANG, E. T.; MARTÍNEZ-ROMERO, E.; CABALLERO-MELLADO, J. Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp.nov., associated with coffee plants. International Journal of Systematic and Evolutionary Microbiology, Reading, v. 51, Part 4, p. 1305-1314, jul. 2001. Biblioteca(s): Embrapa Agrobiologia. |
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18. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, R. A.; ROGEL, M. A.; LÓPEZ-LÓPEZ, A.; ORMEÑO-ORRILLO, E.; BARCELLOS, F. G.; MARTÍNEZ, J.; THOMPSON, F. L.; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. Reclassification of Rhizobium tropical type A strains as Rhizobium leucaenae sp. nov. International Journal of Systematic and Evolutionary Microbiology, Reading, v. 62 , Pt. 5, p. 1179-1184, 2012. Biblioteca(s): Embrapa Soja. |
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19. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, R. A.; MARTINS, T. B.; ORMENO-ORRILLO, E.; DELAMUTA, J. R. M.; ROGEL, M. A.; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. Rhizobium ecuadorense sp. nov., an indigenous N2-fixing symbiont of the Ecuadorian common bean (Phaseolus vulgaris L.) genetic pool. International Journal of Systematic and Evolutionary Microbiology, v. 65, n. 9, p. 3162-3169, Sept. 2015. Biblioteca(s): Embrapa Soja. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DALL'AGNOL, R. F.; RIBEIRO, R. A.; ORMEÑO-ORRILLO, E.; ROGEL, M. A.; DELAMUTA, J. R. M.; MARTÍNEZ-ROMERO, E.; ANDRADE, D. S.; HUNGRIA, M. Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen. International Journal of Systematic and Evolutionary Microbiology, v. 63, n.11, p. 4167-4173, 2013. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 31 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
12/01/2024 |
Data da última atualização: |
12/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRIGIDA, A. I. S.; BUARQUE, F. S.; NOGUEIRA, V. L. R.; MELO, V. M. M.; GUISÁN SEIJAS, J. M.; RIBEIRO, B. D.; GONÇALVES, L. R. B.; COELHO, M. A. Z. |
Afiliação: |
ANA IRAIDY SANTA BRIGIDA, CNPAT; FILIPE SMITH BUARQUE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VANESSA LÚCIA RODRIGUES NOGUEIRA, UNIVERSIDADE FEDERAL DO CEARÁ; VÂNIA MARIA MACIEL MELO, UNIVERSIDADE FEDERAL DO CEARÁ; JOSÉ MANUEL GUISÁN SEIJAS, INSTITUTE OF CATALYSIS AND PETROCHEMISTRY; BERNARDO DIAS RIBEIRO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCIANA ROCHA BARROS GONÇALVES, UNIVERSIDADE FEDERAL DO CEARÁ; MARIA ALICE ZARUR COELHO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
Título: |
Partial purification of crude lipase extract from Yarrowia lipolytica: precipitation, aqueous two-phase systems (ATPS), and immobilization methods. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Cleaner Chemical Engineering, v. 6, 11 p., art. 100105, June 2023. |
DOI: |
10.1016/j.clce.2023.100105 |
Idioma: |
Inglês |
Conteúdo: |
Efforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. |
Palavras-Chave: |
Aqueous biphasic system; Aqueous two-phase system; Sistema aquoso bifásico; Sistema aquoso em duas fases. |
Thesaurus NAL: |
Chemical precipitation; Purification methods; Yarrowia lipolytica. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160760/1/ART-23038.pdf
|
Marc: |
LEADER 02400naa a2200301 a 4500 001 2160760 005 2024-01-12 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1016/j.clce.2023.100105$2DOI 100 1 $aBRIGIDA, A. I. S. 245 $aPartial purification of crude lipase extract from Yarrowia lipolytica$bprecipitation, aqueous two-phase systems (ATPS), and immobilization methods.$h[electronic resource] 260 $c2023 520 $aEfforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethylene glycol (PEG) and potassium phosphate; and direct immobilization. It was impossible to obtain stable precipitates of the enzyme due to the low concentration of total protein and the presence of biosurfactant produced by the microorganism. Different mixture compositions were selected for the partitioning study. Three ATPS showed selective partitioning of the target enzymes, i.e., lipase and protease migrated to opposite phases. In the ATPS composed of 13 wt% PEG-4000 and 10 wt% salts, it was possible to achieve a purification factor for lipase of 4.2. Purification by immobilization performed by lipase-lipase interactions showed three lipases of distinct sizes in the crude extract. In the immobilization method by hydrophobic supports, phenyl-agarose and butyl‑agarose were more selective in immobilizing than octyl-agarose. In the ion exchange immobilization method, only the lipases identified at 66 kDa and 41 kDa have an attraction for DEAE-agarose (anionic) and sulfopropyl-agarose (cationic) matrices. 650 $aChemical precipitation 650 $aPurification methods 650 $aYarrowia lipolytica 653 $aAqueous biphasic system 653 $aAqueous two-phase system 653 $aSistema aquoso bifásico 653 $aSistema aquoso em duas fases 700 1 $aBUARQUE, F. S. 700 1 $aNOGUEIRA, V. L. R. 700 1 $aMELO, V. M. M. 700 1 $aGUISÁN SEIJAS, J. M. 700 1 $aRIBEIRO, B. D. 700 1 $aGONÇALVES, L. R. B. 700 1 $aCOELHO, M. A. Z. 773 $tCleaner Chemical Engineering$gv. 6, 11 p., art. 100105, June 2023.
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