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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/12/2018 |
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Data da última atualização: |
23/06/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. |
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Afiliação: |
ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; Cenargen; EMORY UNIVERSITY; MCGILL UNIVERSITY; DARIO GRATTAPAGLIA, Cenargen; ELIBIO LEOPOLDO RECH FILHO, Cenargen. |
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Título: |
Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We also present our efforts to apply DNA-free editing tools in implementing genetic alterations that are expected to engineer desirable traits in soybeans by modifying meiotic specific expression. These changes are expected to increase genetic and epigenetic variations with favorable outcomes in seed oil and protein pathway regulation. Financial support: Embrapa-Monsanto Fund/Project:03.17.03.002.00.00 MenosABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We ... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Seqüenciamento de longa duração. |
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Thesagro: |
Genoma; Melhoramento Genético Vegetal; Soja. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187384/1/Coupling-in-depth-genome-annotations-with-genome-editing-technology-for-harnessing-genomic-variation-to-promote-precision-breeding-in-tropical-soybean-APAGAR.pdf
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Marc: |
LEADER 02810nam a2200241 a 4500
001 2101385
005 2022-06-23
008 2018 bl uuuu u01u1 u #d
100 1 $aSILVA JUNIOR, O. B. da
245 $aCoupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean.$h[electronic resource]
260 $aIn: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec$c2018
520 $aABSTRACT: Directional selection during crop domestication and advanced breeding has resulted in significant changes in plant genomes. The extent of intraspecific variation in a crop documents domestication and highlights variants underlying complex traits. Studies demonstrate that most naturally occurring variants in crops are low frequency, and only a small fraction of those might have important functions to trait variation. To understand the inheritance of quantitative traits, diversity panels have become available by crossing inbred lines to produce genomic maps that relate phenotypic variation to recombination and ultimately to genome structure. Using these resources, genomics studies have suggested that selection performs poorly in pericentromeric regions because of reduced recombination frequency (RF). Thus, manipulating RF and meiotic crossover location is an appealing issue to advance crop breeding. In our study, we are using genomic analysis to characterize intragenomic diversity of tropical elite soybean lines in terms of heterozygosity, RF, chromatin structure and accessibility. Our results to date include the generation of draft genome assemblies for two soybean advanced lines using PACBIO long-read sequencing, Chromium Linked-Reads sequencing and an approach rooted in polymer physics that determines the most likely genome structure using chromosomal contact data. In-depth genome-wide characterization is ongoing using ATAC-Seq and Parent-Progeny resequencing. We also present our efforts to apply DNA-free editing tools in implementing genetic alterations that are expected to engineer desirable traits in soybeans by modifying meiotic specific expression. These changes are expected to increase genetic and epigenetic variations with favorable outcomes in seed oil and protein pathway regulation. Financial support: Embrapa-Monsanto Fund/Project:03.17.03.002.00.00
650 $aGenoma
650 $aMelhoramento Genético Vegetal
650 $aSoja
653 $aAnálise genômica
653 $aSeqüenciamento de longa duração
700 1 $aPESSOA FILHO, M. A. C. de P.
700 1 $aSA, M. E. L.
700 1 $aDEAL, R.
700 1 $aRAGOUSSIS, I.
700 1 $aGRATTAPAGLIA, D.
700 1 $aRECH FILHO, E. L.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/10/2003 |
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Data da última atualização: |
03/06/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DUTRA, P. R. S.; ASSIS, J. S. de; AZEVEDO, L. C. de; RIBEIRO, A. P. L.; ANDRADE, S. A.; GUERRA, N. B. |
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Afiliação: |
JOSTON SIMAO DE ASSIS, CPATSA. |
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Título: |
Desenvolvimento do fruto da mangueira cv. Tommy Atkins no município de Petrolina, PE, Brasil. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: SEMANA INTERNACIONAL DA FRUTICULTURA, FLORICULTURA E AGROINDÚSTRIA - FRUTAL, 10.; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DA HORTICULTURA TROPICAL, 49., 2003, Fortaleza. Anais... Fortaleza : Instituto Frutal, 2003. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Para um melhor conhecimento do desenvolvimento do fruto foi determinado o comportamento dos parâmetros físicos para estimar a melhor época de colheita. |
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Palavras-Chave: |
Caracteristica; Desenvolvimento; Pernambuco; Petrolina; Vale do São Francisco; Variedade Tommy Atkins. |
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Thesagro: |
Manga. |
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Thesaurus NAL: |
Mangoes. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/27274/1/OPB711.pdf
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Marc: |
LEADER 01101nam a2200277 a 4500
001 1152025
005 2022-06-03
008 2003 bl uuuu u00u1 u #d
100 1 $aDUTRA, P. R. S.
245 $aDesenvolvimento do fruto da mangueira cv. Tommy Atkins no município de Petrolina, PE, Brasil.
260 $aIn: SEMANA INTERNACIONAL DA FRUTICULTURA, FLORICULTURA E AGROINDÚSTRIA - FRUTAL, 10.; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DA HORTICULTURA TROPICAL, 49., 2003, Fortaleza. Anais... Fortaleza : Instituto Frutal$c2003
300 $c1 CD-ROM.
520 $aPara um melhor conhecimento do desenvolvimento do fruto foi determinado o comportamento dos parâmetros físicos para estimar a melhor época de colheita.
650 $aMangoes
650 $aManga
653 $aCaracteristica
653 $aDesenvolvimento
653 $aPernambuco
653 $aPetrolina
653 $aVale do São Francisco
653 $aVariedade Tommy Atkins
700 1 $aASSIS, J. S. de
700 1 $aAZEVEDO, L. C. de
700 1 $aRIBEIRO, A. P. L.
700 1 $aANDRADE, S. A.
700 1 $aGUERRA, N. B.
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Embrapa Semiárido (CPATSA) |
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