Data/hora: 03/06/2024 18:28:04

Biblioteca(s): | Embrapa Hortaliças; Embrapa Unidades Centrais.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | Nacional - B
Data corrente: | 22/11/2004
Data da última atualização: | 15/05/2024
Autoria: | MELO FILHO, P. de A.; NAGATA, T.; DUSI, A. N.; BUSO, J. A.; TORRES, A. C.; EIRAS, M.; RESENDE, R. de O.
Afiliação: | PÉRICLES DE ALBUQUERQUE MELO FILHO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; TATSUYA NAGATA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANDRE NEPOMUCENO DUSI, CNPH; JOSE AMAURI BUSO, CNPH; ANTONIO CARLOS TORRES, CNPH; MARCELO EIRAS, INSTITUTO BIOLÓGICO; RENATO DE OLIVEIRA RESENDE, UNIVERSIDADE DE BRASÍLIA.
Título: | Detection of three Allexivirus species infecting garlic in Brazil.
Ano de publicação: | 2004
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, p. 735-740, ago. 2004.

Idioma:

Inglês
Português

Conteúdo: | Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of Biotechnology Information (NCBI). By these procedures, individual garlic virus genomes were isolated and sequenced. The nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses GarV-C, GarV-D and a recently described allexivirus, named Garlic mite-borne filamentous virus (GarMbFV), in Brazil.
Palavras-Chave: | coat protein; detecção de vírus; Dot-ELISA; proteínas capsidiais; RT-PCR; virus detection.
Thesagro: | Alho; Allium Sativum; Vírus.
Thesaurus Nal: | Garlic mite-borne filamentous virus; Garlic virus A; Garlic virus B; Garlic virus C; Garlic virus D.
Categoria do assunto: | --

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/777154/1/AP-CNPH-2004.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/28996/1/39n08a02.pdf

Registro original: | Embrapa Hortaliças (CNPH)

Exemplares:

AI-SEDE - (28996-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS
CNPH - (29155-1) - UPC - AP - DD - 630.5 -  -  - DIS