Data/hora: 05/06/2024 05:24:32

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 24/01/2006
Data da última atualização: | 25/02/2013
Autoria: | CALIGIORNE, R. B.; RESENDE, M. A.; PAIVA, E.; AZEVEDO, V.
Afiliação: | Embrapa Milho e Sorgo.
Título: | Use of RAPD (random amplified polymorphic DNA) to analyse genetic diversity of dematiaceous fungal pathogens.
Ano de publicação: | 1999
Fonte/Imprenta: | Canadian Journal of Microbiology, Ottawa, v. 45, n. 5, p. 408-412 , 1999.
Idioma: | Inglês
Conteúdo: | A total of 13 strains of fungi, the aetiological agents of chromoblastomycosis and phaeohyphomycosis (including Rhinocladiella sp., R. aquaspersa, Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala dermatitis [Wangiella dermatitidis]) were obtained from different geographical origins. These strains were genotypically compared by random amplified polymorphic DNA (RAPD). The data generated showed a high degree of polymorphism between distinct species and a low polymorphism between strains of the same species. The results generated by these tests were subjected to a numerical taxonomy analysis, using the unweighted pair-group method. A phenogram was constructed for the set of strains studied. Based on its structure, it is concluded that genotypical data provided enough information to use the unweighted pair-group method to cluster the strains in accordance to their respective species. The phenogram grouped in a single branch the strains of Fonsecaea pedrosoi and F. compacta species, indicating a great similarity between these fungi, and suggesting that the classification as distinct species may not be appropriate for these species of the genus Fonsecaea..
Palavras-Chave: | Genetic diversity; Identification; Phaeohyphomycosis; Random amplified polymorphic DNA; Techniques.
Thesagro: | DNA.
Thesaurus Nal: | classification; polymorphism; taxonomy.
Categoria do assunto: | --
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (18281-1) - UPC - AP - DD -  -  -  - DIS