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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
BERGER, I. J.; FREITAS-ÁSTUA, J.; REIS, M. S.; TARGON, M. L. P. N.; MACHADO, M. A. |
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Afiliação: |
Irving J. Berger, APTA; Juliana Freitas-Astúa, CNPMF; Marcelo S. Reis, APTA; Maria Luísa P.N. Targon, APTA; Marcos A. Machado, APTA. |
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Título: |
In silico prediction of gene expression patterns in Citrus flavedo. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 752-760, 2007. |
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ISSN: |
1415-4757 |
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Idioma: |
Inglês |
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Conteúdo: |
Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in flavedo, are shown to be promising candidates for further characterization. In addition to promoter seeking, this kind of analysis can lead to gene discovery, tissue-specific and tissue-enriched expression pattern predictions (as shown herein) and can also be adopted as an in silico first, and probably reliable approach, for detecting expression profiles from EST sequencing efforts before experimental validation is available or for heuristically guiding that validation. |
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Palavras-Chave: |
EST; Fruit; Orange; Tissue-specific. |
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Thesaurus NAL: |
gene expression. |
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Categoria do assunto: |
-- |
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Marc: |
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001 1654655
005 2023-07-24
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022 $a1415-4757
100 1 $aBERGER, I. J.
245 $aIn silico prediction of gene expression patterns in Citrus flavedo.$h[electronic resource]
260 $c2007
520 $aOut of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in flavedo, are shown to be promising candidates for further characterization. In addition to promoter seeking, this kind of analysis can lead to gene discovery, tissue-specific and tissue-enriched expression pattern predictions (as shown herein) and can also be adopted as an in silico first, and probably reliable approach, for detecting expression profiles from EST sequencing efforts before experimental validation is available or for heuristically guiding that validation.
650 $agene expression
653 $aEST
653 $aFruit
653 $aOrange
653 $aTissue-specific
700 1 $aFREITAS-ÁSTUA, J.
700 1 $aREIS, M. S.
700 1 $aTARGON, M. L. P. N.
700 1 $aMACHADO, M. A.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 30, n. 3, p. 752-760, 2007.
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