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| Registros recuperados : 112 | |
| 2. |  | OLIVEIRA, F. R. de; REIS, D. R. de L.; SOUZA SOBRINHO, F. de; AZEVEDO, A. L. S. Transferência de marcadores microssatélites entre espécies do gênero Brachiaria. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | BRION, C. T.; SANTOS, L. B.; REIS, D. R. de L.; MARTINS, M. F.; SILVA, M. V. G. B.; PANETTO, J. C. do C.; MACHADO, M. A. Banco de DNA de Bovinos: avaliação de metodologias alternativas para extração de DNA. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | LANGE, C. C.; BRITO, M. A. V. P. e; SILVA, F. S. DA; ALVIM, M. C. T.; REIS, D. R. de L.; DOMINGUES, R. Identificação de estafilococos coagulase negativos isolados mastite bovina por sequenciamento do rDNA 16S. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçú. Resumos... Foz do Iguaçú: SBM, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | LANGE, C. C.; BRITO, M. A. V. P. e; SILVA, F. S.; ALVIM, M. C. T.; REIS, D. R. de L.; DOMINGUES, R. Identification of coagulase negative staphylococci isolated from bovine mastitis by 16S rDNA sequencing. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 13| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | GOMIDE, M.; LEMOS, F.; REIS, D. R. de L.; JOSÉ, G.; LOPES, M.; MACHADO, M. A.; ALVES, T.; COELHO, C. M. Identification of dysregulated microRNA expression and their potential role in the antiproliferative effect of the essential oils from four different Lippia species against the CT26.WT colon tumor cell line. Revista Brasileira de Farmacognosia, v. 26, n. 5, p. 627-633, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | SABINO, Y. N. V.; RIBEIRO, J. B.; REIS, D. R. de L.; FOCHAT, R. C.; CARNEIRO, J. da C.; RIBEIRO, M. T.; MACHADO, M. A.; PAIVA, A. D. Identification and gene prospection of bacteriocinogenic bacteria isolated from rumen In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Resumos. Florianópolis: Sociedade Brasileira de Microbiologia, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | WOHLRES-VIANA, S.; BERNARDO, K. B.; CUNHA, A. C. L. M.; REIS, D. R. de L.; ARASHIRO, E. K. N.; MACHADO, M. A.; VIANA, J. H. M. Identification of SNPs in the luteinizing hormone receptor transcript of Gyr (Bos indicus) granulosa cells. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçú: Sociedade Brasileira de Genética, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | HOSKEN, B.; BRITO, M. A. V. P. e; REIS, D. R. de L.; SOUZA, G. N. de; ALMEIDA, P.; MACHADO, M. A.; SILVA, M. R.; RIBEIRO, J. B. Investigação da resistência à eritromicina em linhagens de Streptococcus agalactiae isoladas de casos de mastite bovina. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 7., 2017, Curitiba. Anais... Curitiba: Conselho Brasileiro de Qualidade do Leite, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | SANTOS, F. F.; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; LANGE, C. C.; SILVA, M. A. S.; REIS, D. R. de L.; MACHADO, M. A. Presence of mecA in Staphylococcus spp. isolated from bovine intramammary infections. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçú: Sociedade Brasileira de Genética, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.; PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M. Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle. Genetics and Molecular Research, v. 15, n. 2, 2016. 12 p.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | MAGALHÃES, R. L. O. de; NOGUEIRA, A. L.; DOMINGUES, R.; REIS, D. R. de L.; MARTINS, M. F.; MACHADO, M. A.; AZEVEDO, A. L. S. Validação de um painel específico (Fingerprinting) para identificação da cultivar BRS Integra. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 27., 2023, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2024. p. 127-131. (Embrapa Gado de Leite. Eventos Técnicos & Científicos, 2). Pibic/CNPq.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | SEIBERLICK, O. S. G.; CARVALHO, B. O.; NASCIMENTO, J. C.; AZEVEDO, A. L. S.; REIS, D. R. de L.; LEDO, F. J. da S.; MACHADO, M. A. Varredura do banco ativo de germoplasma de capim-elefante para identificação de regiões genômicas associadas a apomixia. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 24., 2019, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2019. 4 p. Editor Técnico: Leônidas Paixão Passos, Embrapa Gado de Leite.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | LANGE, C. C.; BRITO, M. A. V. P. e; REIS, D. R. de L.; MACHADO, M. A.; GUIMARAES, A. S.; AZEVEDO, A. L. S.; SALLES, É. B.; ALVIM, M. C. T.; SILVA, F. S.; MEURER, I. R. Species level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing Veterinary Microbiology, v. 176, n. 3-4, p. 382-85, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | FREGULIA, P.; CAMPOS, M. M.; DIAS, R. J. P.; LIU, J.; GUO, W.; PEREIRA, L. G. R.; MACHADO, M. A.; REIS, D. R. de L.; GUAN, L. L.; GARNSWORTHY, P. C.; NEVES, A. L. A. Taxonomic and predicted functional signatures reveal linkages between the rumen microbiota and feed efficiency in dairy cattle raised in tropical areas. Frontiers in Microbiology, v. 13, 1025173, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | DIAS, A. T.; CASTRO, S. B. R. de; ALVES, C. C. de S.; EVANGELISTA, M. G.; SILVA, L. C. da; REIS, D. R. de L.; MACHADO, M. A.; JULIANO, M. A.; FERREIRA, A. P. Genistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis. Inflammation Research, v. 67, n. 7, p. 597-608, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | PEIXOTO, M. G. C. D.; SANTIAGO, L. C.; STEINBERG, R. S.; GASPARINI, K.; REIS, D. R. de L.; DOMINGUES, R.; EGITO, A. A. do; CARVALHO, M. R. S.; VERNEQUE, R. da S.; MACHADO, M. A.; AZEVEDO, A. L. S. Genetic differentiation of Guzerat (Bos Indicus) metapopulation in Minas Gerais state, Brazil. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 9., 2010, Leipzig. Proceedings... Leipzig: German Society fo Animal Science, 2010. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | SILVA, M. V. G. B.; MARTINS, M. F.; CEMBRANELLI, M. DE A. R.; PANETTO, J. C. do C.; PAIVA, L. DE C.; GONÇALVES, G. S.; MACHADO, M. A.; REIS, D. R. de L.; ALVES, B. R. C. (ed.). Girolando breed genetic improvement program - Sire summary - Progeny test results - June 2018. Juiz de Fora: Embrapa Gado de Leite, 2018. 62 p. (Embrapa Gado de Leite. Documentos, 222)| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | ALVES, A. S.; CAMPOS, R. A.; MALUF, V. H. H. K.; PEREIRA, H. P.; VIEIRA, F. de O.; REIS, D. R. de L.; GUIMARÃES, A. S.; MACHADO, M. A.; MARTINS, M. F. Identificação de vacas da raça Holandesa portadoras de alelo recessivo das doenças genéticas CVM, BLAD e DUMPS e genotipagem para Beta-caseína. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 25., 2021, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2021. (Embrapa Gado de Leite. Documentos, 261).| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 112 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, F. F. dos; MENDONCA, L. C.; REIS, D. R. de L.; GUIMARAES, A. S.; LANGE, C. C.; RIBEIRO, J. B.; MACHADO, M. A.; BRITO, M. A. V. P. e. |
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Afiliação: |
Fernanda Fernandes dos Santos, UFJF; LETICIA CALDAS MENDONCA, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; CARLA CHRISTINE LANGE, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL. |
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Título: |
Presence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 2, p. 1374-1382, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures. MenosAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, ery... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial resistance; MecA; Methicillin-resistant staphylococci; SCCmec. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03425naa a2200253 a 4500
001 2061479
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. F. dos
245 $aPresence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil.$h[electronic resource]
260 $c2016
520 $aAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures.
653 $aAntimicrobial resistance
653 $aMecA
653 $aMethicillin-resistant staphylococci
653 $aSCCmec
700 1 $aMENDONCA, L. C.
700 1 $aREIS, D. R. de L.
700 1 $aGUIMARAES, A. S.
700 1 $aLANGE, C. C.
700 1 $aRIBEIRO, J. B.
700 1 $aMACHADO, M. A.
700 1 $aBRITO, M. A. V. P. e
773 $tJournal of Dairy Science$gv. 99, n. 2, p. 1374-1382, 2016.
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