Data/hora: 15/06/2024 12:40:33

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 30/01/2024
Data da última atualização: | 30/01/2024
Autoria: | REIS, C. B. dos; OTENIO, M. H.; MELO JÚNIOR, A. M. de; DORNELAS, J. C. M.; CARMO, P. H. F. do; VIANA, R. de O.; RICOY, A. C. S.; ALVES, V. de S.
Afiliação: | CAMILA BRANDA DOS REIS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; MARCELO HENRIQUE OTENIO, CNPGL; ANDERSON MACHADO DE MELO JUNIOR, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JOAO CARLOS MAIA DORNELAS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAULO HENRIQUE FONSECA DO CARMO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ROBERTA DE OLIVEIRA VIANA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ANA CAROLINA SANTOS RICOY, UNIVERSIDADE FEDERAL DE MINAS GERAIS; VIVIANE DE SOUZA ALVES, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
Título: | Virulence profile of Candida spp. isolated from an anaerobic biodigester supplied with dairy cattle waste.
Ano de publicação: | 2024
Fonte/Imprenta: | Microbial Pathogenesis, v. 187, 106516, 2024.
DOI: | https://doi.org/10.1016/j.micpath.2023.106516
Idioma: | Inglês
Conteúdo: | Anaerobic biodigesters play a crucial role in enhancing animal waste management. However, the presence of pathogens in the biodigestion process poses a significant concern. Candida spp., a widespread fungus known for its opportunistic nature and adaptability to diverse environmental conditions, including reciprocal transmission between humans and animals, is one such pathogen of concern. Therefore, it is imperative to assess the virulence profile of Candida spp. originating from anaerobic biodigestion processes. Here we demonstrate that strains isolated from the biodigestion process of dairy cattle waste exhibit noteworthy virulence mechanisms, surpassing the virulence of clinical control strains. After we identified strains from affluent, effluent, and biofertilizer, we observed that all analyzed isolates produced biofilm. Additionally, a substantial proportion of these isolates demonstrated phospholipase production, while only a few strains exhibited protease production. Furthermore, all strains exhibited resistance or dose-dependent responses to amphotericin B and itraconazole, with the majority displaying resistance to fluconazole. In the in vivo test, we observed a significant correlation (p < 0.05) between the LT50 and biofilm formation as well as hyphae/pseudohyphae production. Additionally, some isolates demonstrated a quicker nematode-killing capacity compared to clinical controls. Our findings underscore the considerable pathogenic potential of certain Candida species present in the dynamics of anaerobic biodigestion. Importantly, the anaerobic biodigester system did not eliminate Candida strains from dairy cattle waste, highlighting the need for caution in utilizing biodigester products. We advocate for further studies to explore the virulence of other microorganisms in various animal production contexts. Furthermore, our results emphasize the urgency of enhancing waste treatment methods to effectively eliminate pathogens and curb their potential dissemination.
Palavras-Chave: | Antifúngico; Biodigestão; Levedura; Resíduo animal.
Thesagro: | Biodigestor; Fungo; Gado Leiteiro; Resíduo.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (26276-1) - UPC - AP - DD -  -  -  - DIS