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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/08/2018 |
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Data da última atualização: |
15/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, F. A. de; SILVA, I. R. da; BARRETO, M. B. B. de B.; OEHL, F.; GOTO, B. T.; MAIA, L. C. |
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Afiliação: |
FRANCISCO ADRIANO DE SOUZA, CNPMS; Iolanda Ramalho da Silva, Universidade Federal de Pernambuco; Maria Beatriz Barbosa de Barros Barreto, Universidade Federal do Rio de Janeiro; Fritz Oehl, Agroscope, Competence Division for Plants and Plant Products, Ecotoxicology, Schloss; Bruno Tomio Goto, Universidade Federal do Rio Grande do Norte; Leonor Costa Maia, Universidade Federal de Pernambuco. |
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Título: |
Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Mycological Progress, v. 17, p. 999-1011, 2018. |
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DOI: |
10.1007/s11557-018-1410-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Here, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long continuous nuclear rDNA sequence fragment, which encompasses the nearly complete 18S SSU sequence gene until the 3? end of the D2 region of the 28S LSU (~ 3100 bp), which allows for comparison with sequences obtained from regions used for fungal metagenomic studies, species description, and AMfungi DNA-barcode. The new species forms dark brown to black spores, approx. 340?510 ?m on in diam., on sporogenous cells. The spores have unique Bcloud/flower^ projections on the spore surface, two walls, and differentiate a multiple-lobed germ shield with up to 8?12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0?2 %) is within the lower range for the genus. The V3?V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from this fragment, we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from reference strains and those obtained from environmental sequences in Glomeromycotina is still a problematic issue, mainly due to the reduced number of AM fungal species characterized based on DNA sequences. MenosHere, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long continuous nuclear rDNA sequence fragment, which encompasses the nearly complete 18S SSU sequence gene until the 3? end of the D2 region of the 28S LSU (~ 3100 bp), which allows for comparison with sequences obtained from regions used for fungal metagenomic studies, species description, and AMfungi DNA-barcode. The new species forms dark brown to black spores, approx. 340?510 ?m on in diam., on sporogenous cells. The spores have unique Bcloud/flower^ projections on the spore surface, two walls, and differentiate a multiple-lobed germ shield with up to 8?12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0?2 %) is within the lower range for the genus. The V3?V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from this fragment, we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from reference strains and those obtained from environmental sequences in Glomeromycotina is still a ... Mostrar Tudo |
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Thesagro: |
DNA; Filogenia; Fungo; Micorriza. |
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Thesaurus Nal: |
Mycorrhizae. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02371naa a2200253 a 4500
001 2094151
005 2018-08-15
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11557-018-1410-9$2DOI
100 1 $aSOUZA, F. A. de
245 $aRacocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny.$h[electronic resource]
260 $c2018
520 $aHere, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long continuous nuclear rDNA sequence fragment, which encompasses the nearly complete 18S SSU sequence gene until the 3? end of the D2 region of the 28S LSU (~ 3100 bp), which allows for comparison with sequences obtained from regions used for fungal metagenomic studies, species description, and AMfungi DNA-barcode. The new species forms dark brown to black spores, approx. 340?510 ?m on in diam., on sporogenous cells. The spores have unique Bcloud/flower^ projections on the spore surface, two walls, and differentiate a multiple-lobed germ shield with up to 8?12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0?2 %) is within the lower range for the genus. The V3?V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from this fragment, we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from reference strains and those obtained from environmental sequences in Glomeromycotina is still a problematic issue, mainly due to the reduced number of AM fungal species characterized based on DNA sequences.
650 $aMycorrhizae
650 $aDNA
650 $aFilogenia
650 $aFungo
650 $aMicorriza
700 1 $aSILVA, I. R. da
700 1 $aBARRETO, M. B. B. de B.
700 1 $aOEHL, F.
700 1 $aGOTO, B. T.
700 1 $aMAIA, L. C.
773 $tMycological Progress$gv. 17, p. 999-1011, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
31/08/2017 |
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Data da última atualização: |
31/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
REGO, E. C. P. DO; GUIMARÃES, E. DE F.; RODRIGUES, J. M.; SCARLATO, R. C.; NOGUEIRA, R. I.; PEREIRA NETTO, A. D. |
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Afiliação: |
Eliane Cristina Pires do Rego, INMETRO; Evelyn de Freitas Guimarães, INMETRO; Janaína Marques Rodrigues, INMETRO; Renata Cristina Scarlato, INMETRO; REGINA ISABEL NOGUEIRA, CTAA; Annibal Duarte Pereira Netto, UFF. |
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Título: |
Feasibility study for development of candidate reference material for food analysis: Chloramphenicol in milk powder. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Measurement, n. 98, p. 300-304, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Reliable analytical results are necessary to ensure effective consumer protection and the use of certified reference material (CRM) is an important tool for quality assurance. The Brazilian Metrology Institute (Inmetro) studied the feasibility of the production of a candidate CRM of chloramphenicol (CAP) in milk. CAP is an antibiotic banned for treatment of food-producing animals. A minimum required performance limit (MRPL) for analytical methods of 0.3 lg kg1 was set by the European Community for the detection of CAP residues in food matrices. Liquid chromatography with isotope dilution mass spectrometry (LC? IDMS) was employed for CAP determination and water content was measured by Karl Fischer coulometric titration. The feasibility study showed milk powder processed with both spray dryer and lyophilization lead to the best results, showing good degree of inhomogeneity for CAP residues (5.0%) and for water content (3.0%), therefore demonstrating that the production of this candidate CRM was feasible |
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Palavras-Chave: |
Feasibility study; Homogeneity Certified Reference Material (CRM). |
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Thesaurus NAL: |
chloramphenicol; milk. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01741naa a2200229 a 4500
001 2074788
005 2017-08-31
008 2017 bl uuuu u00u1 u #d
100 1 $aREGO, E. C. P. DO
245 $aFeasibility study for development of candidate reference material for food analysis$bChloramphenicol in milk powder.$h[electronic resource]
260 $c2017
520 $aReliable analytical results are necessary to ensure effective consumer protection and the use of certified reference material (CRM) is an important tool for quality assurance. The Brazilian Metrology Institute (Inmetro) studied the feasibility of the production of a candidate CRM of chloramphenicol (CAP) in milk. CAP is an antibiotic banned for treatment of food-producing animals. A minimum required performance limit (MRPL) for analytical methods of 0.3 lg kg1 was set by the European Community for the detection of CAP residues in food matrices. Liquid chromatography with isotope dilution mass spectrometry (LC? IDMS) was employed for CAP determination and water content was measured by Karl Fischer coulometric titration. The feasibility study showed milk powder processed with both spray dryer and lyophilization lead to the best results, showing good degree of inhomogeneity for CAP residues (5.0%) and for water content (3.0%), therefore demonstrating that the production of this candidate CRM was feasible
650 $achloramphenicol
650 $amilk
653 $aFeasibility study
653 $aHomogeneity Certified Reference Material (CRM)
700 1 $aGUIMARÃES, E. DE F.
700 1 $aRODRIGUES, J. M.
700 1 $aSCARLATO, R. C.
700 1 $aNOGUEIRA, R. I.
700 1 $aPEREIRA NETTO, A. D.
773 $tMeasurement$gn. 98, p. 300-304, 2017.
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Embrapa Agroindústria de Alimentos (CTAA) |
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