Data/hora: 03/06/2024 01:19:42

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 16/02/2005
Data da última atualização: | 11/05/2022
Autoria: | FUNES-HUACCA, M.; REGITANO, L. C. de A.; MURLLER, O.; CARILLHO, E.
Afiliação: | MARIBEL FUNES-HUACCA, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; ODILO MUELLER, Agilent Technologies, Waldbronn, Germany; EMANUEL CARRILHO, USP.
Título: | Semiquantitative determination of Alicyclobacillus acidoterrestris in orange juice by reverse- transcriptase polymerase chain reaction and capillary electrophoresis ? laser induced fluorescence using microchip technology
Ano de publicação: | 2004
Fonte/Imprenta: | Electrophoresis, v.25, n.21-22, p.3860-3864, 2004.
DOI: | https://doi.org/10.1002/elps.200406105
Idioma: | Inglês
Conteúdo: | The semiquantitative detection ofAlicyclobacillus acidoterrrestrisin orange juice byreverse-transcriptase polymerase chain reaction (RT-PCR) with a linear dynamic rangeof 26105– 2 colony forming units (CFU)/mL in terms of cell count is described.Separation, detection, and quantification of the RT-PCR products were accomplishedusing the Agilent 2100 bioanalyzer in conjunction with the DNA 1000 LabChip kit. After0 and 12 h of enrichment, it was possible to generate a linear standard curve betweenthe amount of cells and amplicon concentration of RT-PCR and PCR products. Usingthis method, cell diminution was verified in samples of orange juice treated with a nat-ural inhibitor (Sapindus saponaria), determining the persistence of viable cells. Semi-quantitative RT-PCR using the Agilent 2100 bioanalyzer is a potentially useful approachfor rapidin vitrodetermination ofA. acidoterrestrisand monitoring of inhibitor sus-ceptibility for the orange juice-producing industry.
Palavras-Chave: | Miniaturization; Reversetranscriptase.
Thesaurus Nal: | Alicyclobacillus; Alicyclobacillus acidoterrestris; Microchip technology; Orange juice; Reverse transcriptase polymerase chain reaction.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (15443-1) - UPC - AP - DD - PROCI-2004.00169 - HUA - 2004.00169 - DIS