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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, R. S.; GARCIA, G. M.; VILELA, J. M. C.; ANDRADE, M. S.; OLIVEIRA, L. A. M.; KANO, E. K.; LANGE, C. C.; BRITO, M. A. V. P. E; BRANDAO, H. de M.; MOSQUEIRA, V. C. F. |
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Afiliação: |
RAQUEL SILVA ARAÚJO; GIANI MARTINS GARCIA; JOSÉ MARIO CARNEIRO VILELA; MARGARETH SPANGLER ANDRADE; LASER ANTÔNIO MACHADO OLIVEIRA; EUNICE KAZUE KANO; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; HUMBERTO DE MELLO BRANDAO, CNPGL; VANESSA CARLA FURTADO MOSQUEIRA. |
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Título: |
Cloxacillin benzathine-loaded polymeric nanocapsules: Physicochemical characterization, cell uptake, and intramammary antimicrobial effect. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Materials Science & Engineering C, v. 104, 110006, 2019. |
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DOI: |
https://doi.org/10.1016/j.msec.2019.110006 |
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Idioma: |
Inglês |
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Conteúdo: |
The present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control. MenosThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tra... Mostrar Tudo |
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Palavras-Chave: |
Cell uptake; Cloxacillin benzathine; Intramammary; Release kinetics. |
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Thesaurus Nal: |
Mastitis; Nanocapsules. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02882naa a2200313 a 4500
001 2117206
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.msec.2019.110006$2DOI
100 1 $aARAÚJO, R. S.
245 $aCloxacillin benzathine-loaded polymeric nanocapsules$bPhysicochemical characterization, cell uptake, and intramammary antimicrobial effect.$h[electronic resource]
260 $c2019
520 $aThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control.
650 $aMastitis
650 $aNanocapsules
653 $aCell uptake
653 $aCloxacillin benzathine
653 $aIntramammary
653 $aRelease kinetics
700 1 $aGARCIA, G. M.
700 1 $aVILELA, J. M. C.
700 1 $aANDRADE, M. S.
700 1 $aOLIVEIRA, L. A. M.
700 1 $aKANO, E. K.
700 1 $aLANGE, C. C.
700 1 $aBRITO, M. A. V. P. E
700 1 $aBRANDAO, H. de M.
700 1 $aMOSQUEIRA, V. C. F.
773 $tMaterials Science & Engineering C$gv. 104, 110006, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
01/02/2018 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
VILELA, E. F.; RECH FILHO, E. L.; MARTHA JUNIOR, G. B.; ALVES, E. R. de A.; LOPES, M. A.; GUIMARAES, E. P.; CABRAL, P. R.; SOARES, C. O.; ROSINHA, G. M. S.; BUAINAIN, A. M.; NUTTI, M. R.; CALEGAR, G. M. |
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Afiliação: |
Evaldo Ferreira Vilela, UFV; ELIBIO LEOPOLDO RECH FILHO, Cenargen; GERALDO BUENO MARTHA JUNIOR, CNPTIA; ELISEU ROBERTO DE ANDRADE ALVES, DE/PR; MAURICIO ANTONIO LOPES, CNPAE; ELCIO PERPETUO GUIMARAES, CNPAF; Paulo Renato Cabral; CLEBER OLIVEIRA SOARES, DEIT; GRACIA MARIA SOARES ROSINHA, CNPGC; Antônio Marcio Buainain; MARILIA REGINI NUTTI, CTAA; GERALDO MAGELA CALEGAR, CNPGL. |
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Título: |
Food and Nutrition Security in Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: IANAS. Challenges and opportunities for food and nutrition security in the Americas: The view of the Academies of Sciences. Ciudad de Mexico: IANAS, 2017. p. 76-109. |
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ISBN: |
978-607-8379-29-3 |
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Idioma: |
Inglês |
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Notas: |
IANAS Regional Report november 2017. Tít. em espanhol: Food and Nutrition Security in Brazil. In: Retos y oportunidades de la seguridad alimentaria y nutricional en las Américas: El punto de vista de las Academias de Ciencias. |
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Conteúdo: |
In the past 40 years, the agricultural public and private sectors of Brazil have been working in close collaboration, to promote one of the most impressive and successful sustainable agricultural developments in a middle income country. Brazil has become an example of a food secure country and one the of world?s most important agricultural export countries. Mention should be made of the outstanding role played by the agricultural research technology developed by Brazilian research organizations, led by the agricultural research system encompassing agricultural universities, the Brazilian Agricultural Research Organization (EMBRAPA) and the state agricultural research organizations. This comprehensive executive summary outlines the future challenges and opportunities for the Brazilian agricultural sector in terms of science, technology and innovation, to keep agriculture improving its performance in a world that faces the enormous challenge of feeding a hungry population now and in the following decades. These challenges and opportunities were identified by a select group of highly qualified Brazilian researchers who have spent a lifetime generating and adapting new technology for the development of the Brazilian agriculture sector. |
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Thesagro: |
Nutrição Humana; Segurança Alimentar. |
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Thesaurus NAL: |
Food and Human Nutrition; Food security. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 02481naa a2200325 a 4500
001 2086913
005 2020-03-05
008 2017 bl uuuu u00u1 u #d
020 $a978-607-8379-29-3
100 1 $aVILELA, E. F.
245 $aFood and Nutrition Security in Brazil.$h[electronic resource]
260 $c2017
500 $aIANAS Regional Report november 2017. Tít. em espanhol: Food and Nutrition Security in Brazil. In: Retos y oportunidades de la seguridad alimentaria y nutricional en las Américas: El punto de vista de las Academias de Ciencias.
520 $aIn the past 40 years, the agricultural public and private sectors of Brazil have been working in close collaboration, to promote one of the most impressive and successful sustainable agricultural developments in a middle income country. Brazil has become an example of a food secure country and one the of world?s most important agricultural export countries. Mention should be made of the outstanding role played by the agricultural research technology developed by Brazilian research organizations, led by the agricultural research system encompassing agricultural universities, the Brazilian Agricultural Research Organization (EMBRAPA) and the state agricultural research organizations. This comprehensive executive summary outlines the future challenges and opportunities for the Brazilian agricultural sector in terms of science, technology and innovation, to keep agriculture improving its performance in a world that faces the enormous challenge of feeding a hungry population now and in the following decades. These challenges and opportunities were identified by a select group of highly qualified Brazilian researchers who have spent a lifetime generating and adapting new technology for the development of the Brazilian agriculture sector.
650 $aFood and Human Nutrition
650 $aFood security
650 $aNutrição Humana
650 $aSegurança Alimentar
700 1 $aRECH FILHO, E. L.
700 1 $aMARTHA JUNIOR, G. B.
700 1 $aALVES, E. R. de A.
700 1 $aLOPES, M. A.
700 1 $aGUIMARAES, E. P.
700 1 $aCABRAL, P. R.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
700 1 $aBUAINAIN, A. M.
700 1 $aNUTTI, M. R.
700 1 $aCALEGAR, G. M.
773 $tIn: IANAS. Challenges and opportunities for food and nutrition security in the Americas: The view of the Academies of Sciences. Ciudad de Mexico: IANAS, 2017. p. 76-109.
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Embrapa Agroindústria de Alimentos (CTAA) |
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