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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D. |
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Afiliação: |
Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba. |
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Título: |
Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Annals of Microbiology, v. 69, p. 131-138, 2019. |
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DOI: |
10.1007/s13213-018-1407-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. |
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Palavras-Chave: |
Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp. |
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Thesagro: |
Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02399naa a2200337 a 4500
001 2108297
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s13213-018-1407-2$2DOI
100 1 $aSABINO, Y. N. V.
245 $aAntibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.$h[electronic resource]
260 $c2019
520 $aAbstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem.
650 $aRúmen
653 $aAntibacterial activity
653 $aBacteriocin
653 $aLanC
653 $aLantibiotic
653 $aStreptococcus sp
700 1 $aFOCHAT, R. C.
700 1 $aLIMA, J. C. F.
700 1 $aRIBEIRO, M. T.
700 1 $aARCURI, P. B.
700 1 $aCARNEIRO, J. da C.
700 1 $aMACHADO, M. A.
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, A. B. F.
700 1 $aHÚNGARO, H. M.
700 1 $aRIBEIRO, J. B.
700 1 $aPAIVA, A. D.
773 $tAnnals of Microbiology$gv. 69, p. 131-138, 2019.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
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Data corrente: |
28/08/2023 |
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Data da última atualização: |
06/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOUSA, T. F.; REÇA, B. N. P. V.; CASTRO, G. S.; SILVA, I. J. S. da; CANIATO, F. F.; JÚNIOR, M. B. de A.; YAMAGISHI, M. E. B.; KOOLEN, H. H. F.; BATAGLION, G. A.; HANADA, R. E.; SILVA, G. F. da. |
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Afiliação: |
THIAGO FERNANDES SOUSA, UNIVERSIDADE FEDERAL DO AMAZONAS; BRUNA NAYARA PANTOJA VIEIRA REÇA, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; GLEUCINEI SANTOS CASTRO, UNIVERSIDADE DO ESTADO DO AMAZONAS; INGRIDE JARLINE SANTOS DA SILVA, UNIVERSIDADE FEDERAL DO AMAZONAS; FERNANDA FÁTIMA CANIATO, UNIVERSIDADE FEDERAL DO AMAZONAS; MOYSÉS BATISTA DE ARAÚJO JÚNIOR, UNIVERSIDADE FEDERAL DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; HECTOR HENRIQUE FERREIRA KOOLEN, UNIVERSIDADE FEDERAL DO AMAZONAS; GIOVANA ANCESKI BATAGLION, UNIVERSIDADE FEDERAL DO AMAZONAS; ROGÉRIO EIJI HANADA, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Trichoderma agriamazonicum sp. nov. (Hypocreaceae), a new ally in the control of phytopathogens. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Microbiological Research, v. 275, 127469, Oct. 2023. |
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ISSN: |
1618-0623 |
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DOI: |
https://doi.org/10.1016/j.micres.2023.127469 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all Trichoderma spp. analyzed, while the presence of licheninase GH12 was observed only in T. agriamazonicum. Genome mining analysis identified 33 biosynthetic gene clusters (BGCs) of which 27 are new or uncharacterized, and the main BGCs are related to the production of polyketides. These results demonstrate the potential of this newly described species for agriculture and biotechnology. MenosAbstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all ... Mostrar Tudo |
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Palavras-Chave: |
Amazon fungi; Biocontrol; Biocontrole; Fitopatógenos; Fungos amazônicos; Peptaibols; Peptídeos antibióticos; Phytopathogens; Post-harvest; Trichoderma agriamazonicum. |
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Thesagro: |
Agricultura; Pós-Colheita. |
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Thesaurus NAL: |
Agriculture; Hypocreaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03140naa a2200433 a 4500
001 2156145
005 2023-09-06
008 2023 bl uuuu u00u1 u #d
022 $a1618-0623
024 7 $ahttps://doi.org/10.1016/j.micres.2023.127469$2DOI
100 1 $aSOUSA, T. F.
245 $aTrichoderma agriamazonicum sp. nov. (Hypocreaceae), a new ally in the control of phytopathogens.$h[electronic resource]
260 $c2023
520 $aAbstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all Trichoderma spp. analyzed, while the presence of licheninase GH12 was observed only in T. agriamazonicum. Genome mining analysis identified 33 biosynthetic gene clusters (BGCs) of which 27 are new or uncharacterized, and the main BGCs are related to the production of polyketides. These results demonstrate the potential of this newly described species for agriculture and biotechnology.
650 $aAgriculture
650 $aHypocreaceae
650 $aAgricultura
650 $aPós-Colheita
653 $aAmazon fungi
653 $aBiocontrol
653 $aBiocontrole
653 $aFitopatógenos
653 $aFungos amazônicos
653 $aPeptaibols
653 $aPeptídeos antibióticos
653 $aPhytopathogens
653 $aPost-harvest
653 $aTrichoderma agriamazonicum
700 1 $aREÇA, B. N. P. V.
700 1 $aCASTRO, G. S.
700 1 $aSILVA, I. J. S. da
700 1 $aCANIATO, F. F.
700 1 $aJÚNIOR, M. B. de A.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aKOOLEN, H. H. F.
700 1 $aBATAGLION, G. A.
700 1 $aHANADA, R. E.
700 1 $aSILVA, G. F. da
773 $tMicrobiological Research$gv. 275, 127469, Oct. 2023.
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