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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
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Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
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Palavras-Chave: |
ERIC-PCR; RAPD. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
caseous lymphadenitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02507naa a2200265 a 4500
001 1910089
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI
100 1 $aGUIMARAES, A. S.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource]
260 $c2011
520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
650 $acaseous lymphadenitis
650 $aCorynebacterium Pseudotuberculosis
653 $aERIC-PCR
653 $aRAPD
700 1 $aDORNELES, E. M. S.
700 1 $aANDRADE, G. I.
700 1 $aLAGE, A. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
700 1 $aGOUVEIA, A. M. G.
700 1 $aHEINEMANN, M. B.
773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
06/08/2008 |
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Data da última atualização: |
21/06/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
RASSINI, J. B.; FERREIRA, R. de P.; FREITAS, A. R. de; VILELA, D. |
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Afiliação: |
Joaquim Bartolomeu Rassini, CPPSE; REINALDO DE PAULA FERREIRA, CPPSE; Alfredo Ribeiro de Freitas, CPPSE; DUARTE VILELA, CNPGL. |
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Título: |
Produção de sementes de alfafa (Medicago sativa) na região Sudeste do Brasil. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras, MG. Anais... Lavras: SBZ: UFLA, 2008 |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Em um experimento conduzido na Embrapa Pecuária Sudeste, São Carlos, SP, avaliou-se o potencial de produção de sementes da alfafa LE N 4, na região Sudeste do Brasil. O rendimento e a qualidade das sementes foram medidos em 20 parcelas de 3m2, distribuídas ao acaso em uma área irrigada de 2.000m2. O rendimento médio foi de 421 kg/ha de sementes de alfafa. Concluiu-se que a região tem potencial para produzir sementes dessa forrageira. |
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Palavras-Chave: |
Sementes; Sudeste do Brasil. |
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Thesagro: |
Alfafa; Medicago Sativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43314/1/PROCIJBR2008.00027.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596753/1/Producao-de-sementes-de-alfafa-na-regiao-Sudeste-do-Brasil.pdf
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Marc: |
LEADER 01101nam a2200205 a 4500
001 1040310
005 2021-06-21
008 2008 bl uuuu u00u1 u #d
100 1 $aRASSINI, J. B.
245 $aProdução de sementes de alfafa (Medicago sativa) na região Sudeste do Brasil.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras, MG. Anais... Lavras: SBZ: UFLA$c2008
300 $c1 CD-ROM.
520 $aEm um experimento conduzido na Embrapa Pecuária Sudeste, São Carlos, SP, avaliou-se o potencial de produção de sementes da alfafa LE N 4, na região Sudeste do Brasil. O rendimento e a qualidade das sementes foram medidos em 20 parcelas de 3m2, distribuídas ao acaso em uma área irrigada de 2.000m2. O rendimento médio foi de 421 kg/ha de sementes de alfafa. Concluiu-se que a região tem potencial para produzir sementes dessa forrageira.
650 $aAlfafa
650 $aMedicago Sativa
653 $aSementes
653 $aSudeste do Brasil
700 1 $aFERREIRA, R. de P.
700 1 $aFREITAS, A. R. de
700 1 $aVILELA, D.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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