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Registros recuperados : 138 | |
85. | | SILVA, C. M. da; VASCONCELOS, S. S.; RAMOS, E. M. L. S.; CAPELA, C. Variação temporal do efluxo de dióxido de carbono pelo solo em sistemas de cultivo de dendê consorciado em Tomé-Açu, PA. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa. Biblioteca(s): Embrapa Amazônia Oriental. |
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89. | | ARAÚJO, M. I.; SOUSA, S. G. A. de; RAMOS, E. de M.; MENEZES, M. A. O. de. Aspectos da produção agrosilvicultural e agroextrativista na Hinterlândia Amazônica. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 160-161. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. Biblioteca(s): Embrapa Amazônia Ocidental. |
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90. | | RAMOS, E. J. A.; VIÉGAS, I. de J. M.; SATO, T.; PAULA, M. L. de; THOMAZ, M. A. A. Efeito da adubação NPK em plantas jovens de paricá (Schizolobium amazonicum, Herb) Ducke. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 26.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 5., 2004, Lages. Fertbio 2004: [anais]. Lages: SBCS, 2004. Biblioteca(s): Embrapa Amazônia Oriental. |
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92. | | FERREIRA, M. A. J. F.; BIANCHINI, P. C.; CARVALHO NETO, M. F.; SANTOS, R. R. dos; RAMOS, E. R. Ferramentas participativas para diagnóstico da agrobiodiversidade e identificação de agricultores guardiões Cadernos de Agroecologia, v. 10, n. 3, out. 2015. Edição dos Resumos do IX Congresso Brasileiro de Agroecologia e IV Seminário Estadual de Agroecologia, Belém, PA, set./out. 2015. Biblioteca(s): Embrapa Semiárido. |
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93. | | FARIA, M. T. de; ATHANAZIO, D. A.; RAMOS, E. A. G.; SILVA, E. F.; REIS, M. G.; KO, A. I. Morphological alterations in the kidney of rats with natural and experimental leptospira infection. Journal of Comparative Pathology, v. 137, n. 4, p. 231-238, Nov. 2007. Biblioteca(s): Embrapa Amazônia Oriental. |
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94. | | MARTINS, R. de J.; VESCHI, J. L. A.; RAMOS, E. M.; FREITAS, M. do C.; ATAÍDE, J. Avaliação da ocorrência de anticorpos Anti-brucella abortus em caprinos da região semiárida do Estado de Pernambuco. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 4., 2009, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2009. p. 268-272. (Embrapa Semi-Árido. Documentos, 221). Biblioteca(s): Embrapa Semiárido. |
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97. | | ARAUJO, K. S.; CARDOSO, K. G. V.; PEIXOTO, C. C.; RAMOS, E.; SILVA, H. S. A.; TRINDADE, A. V. Promoção do crescimento em mudas micropropagadas de bananeira por rizobactérias. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1218 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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98. | | ANDREA, M. V.; GOMES, M. V. M.; MARCONDES, C. R.; OLIVEIRA, K. N.; RAMOS, E. S.; FONTELES, S. B. Relação entre polimorfismo do gene do hormônio do crescimento e características de precocidade em novilhas da raça Nelore. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 63, n. 1, p. 153-157, fev. 2011. Biblioteca(s): Embrapa Pecuária Sudeste. |
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99. | | VESCHI, J. L. A.; LANDIM, A. M. de S.; RAMOS, E. M.; ZAFALON, L. F.; CASTRO, R. S. de. Soroprevalência de lentiviroses de pequenos ruminantes em ovinos e caprinos na região do submédio do Vale do São Francisco, Pernambuco, Brasil. Acta Scientiae Veterinariae, v. 40, p. s99, 2012. Suplemento 2. Edição do 1 Encontro Nacional de Epidemiologia Veterinária - ENEPI, São Paulo, jul. 2012. Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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100. | | RAMOS, E. Z.; MIDORIKAWA, G. E. O.; FAVARO, L. C. de L.; SILVA, A. S. da; GOTTSCHALK, L. M. F.; BON, E. P. S. Agrobacterium tumefaciens-mediated transformation of Trichoderma harzianum CFAM-422 using an herbicide resistance gene as selection marker. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59006. Biblioteca(s): Embrapa Agroenergia; Embrapa Agroindústria de Alimentos. |
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Registros recuperados : 138 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
29/04/2021 |
Data da última atualização: |
29/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MELO, L. H. V. de; ROCHA, F. Y. O.; VIDAL, M. S.; GITAHY, P. de M.; ARRUDA, G. M.; BARRETO, C. P.; ALVES, P. B.; RAMOS, E. T. de A. |
Afiliação: |
LEONA HENRIQUE VARIAL DE MELO, UFRJ; FRANCINE YURIKO OTSUKA ROCHA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; PATRICIA DE MEDEIROS GITAHY, CNPAB; GABRIEL MARTINS ARRUDA, UFRRJ; CAMILA PAES BARRETO, UFRRJ; PABLO BELA ALVES, UFRRJ; ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ. |
Título: |
Diversity and biotechnological potential of endophytic Bacillus species originating from the stem apoplast fluid of sugarcane plants. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Applied Soil Ecology, V. 166, 103985, 2021. |
ISSN: |
0929-1393 |
DOI: |
https://doi.org/10.1016/j.apsoil.2021.103985 R |
Idioma: |
Inglês |
Conteúdo: |
The genus Bacillus has been isolated from a wide range of environments and comprises species with biotechnological applications, such as bioinseticides, biofungicides, and biofertilizers. This study investigated the endophytic occurrence and diversity of Bacilli in the apoplastic fluid of sugarcane cultivated in Brazil. Their biocontrol activity against fungi and their plant growth promotion (PGP) potential in sugarcane mini-set plants were also evaluated. Forty-two Bacillus colonies were isolated from the apoplastic fluid of one-year-old commercial sugarcane varieties and analyzed by sequencing the 16S rRNA gene. The ability to produce indols and siderophores and to solubilize phosphate and zinc were evaluated in vitro, along with their cellulose and protease activity and, antagonistic activity against sugarcane phytopatogenic fungi. The PGP potential was determined using strains which were inoculated in sugarcane mini-set plants grown in sterile substrate and later in unsterilized soil pots. The 16S rRNA analyses demonstrated high bacterial diversity with the majority closely related to B. megaterium followed by B. aryabhattai and B. cereus. B. pumilus, B. velezensis, B. safensis, B. flexus and Paenibacillus taichungensis species were also identified. Most strains were able to produce indol, solubilize zinc oxide and presented cellulase activity, while phosphate solubilization and siderophore production was observed in only few strains. The strains LMC21, LMC44 and LMC48 presented high biocontrol activity against the sugarcane fungi Colletotrichum falcatum. The spore formation was observed for strains grown for 8 days in the HCT medium but not in presence of sugarcane juice. Some strains also accumulated higher biomass in sugarcane (sterile conditions) and a mixture of three Bacillus strains performed better in the soil-pot inoculation experiment. These findings show for the first time that many Bacillus species are present in the apoplastic fluid of sugarcane grown in Brazil and indicate that some strains have PGP and biocontrol potential for application as biofertilizer in sugarcane plants. MenosThe genus Bacillus has been isolated from a wide range of environments and comprises species with biotechnological applications, such as bioinseticides, biofungicides, and biofertilizers. This study investigated the endophytic occurrence and diversity of Bacilli in the apoplastic fluid of sugarcane cultivated in Brazil. Their biocontrol activity against fungi and their plant growth promotion (PGP) potential in sugarcane mini-set plants were also evaluated. Forty-two Bacillus colonies were isolated from the apoplastic fluid of one-year-old commercial sugarcane varieties and analyzed by sequencing the 16S rRNA gene. The ability to produce indols and siderophores and to solubilize phosphate and zinc were evaluated in vitro, along with their cellulose and protease activity and, antagonistic activity against sugarcane phytopatogenic fungi. The PGP potential was determined using strains which were inoculated in sugarcane mini-set plants grown in sterile substrate and later in unsterilized soil pots. The 16S rRNA analyses demonstrated high bacterial diversity with the majority closely related to B. megaterium followed by B. aryabhattai and B. cereus. B. pumilus, B. velezensis, B. safensis, B. flexus and Paenibacillus taichungensis species were also identified. Most strains were able to produce indol, solubilize zinc oxide and presented cellulase activity, while phosphate solubilization and siderophore production was observed in only few strains. The strains LMC21, LMC44 and LMC48 p... Mostrar Tudo |
Palavras-Chave: |
Endophytes Taxonomy; Mix inoculant. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02938naa a2200253 a 4500 001 2131551 005 2021-04-29 008 2021 bl uuuu u00u1 u #d 022 $a0929-1393 024 7 $ahttps://doi.org/10.1016/j.apsoil.2021.103985 R$2DOI 100 1 $aMELO, L. H. V. de 245 $aDiversity and biotechnological potential of endophytic Bacillus species originating from the stem apoplast fluid of sugarcane plants.$h[electronic resource] 260 $c2021 520 $aThe genus Bacillus has been isolated from a wide range of environments and comprises species with biotechnological applications, such as bioinseticides, biofungicides, and biofertilizers. This study investigated the endophytic occurrence and diversity of Bacilli in the apoplastic fluid of sugarcane cultivated in Brazil. Their biocontrol activity against fungi and their plant growth promotion (PGP) potential in sugarcane mini-set plants were also evaluated. Forty-two Bacillus colonies were isolated from the apoplastic fluid of one-year-old commercial sugarcane varieties and analyzed by sequencing the 16S rRNA gene. The ability to produce indols and siderophores and to solubilize phosphate and zinc were evaluated in vitro, along with their cellulose and protease activity and, antagonistic activity against sugarcane phytopatogenic fungi. The PGP potential was determined using strains which were inoculated in sugarcane mini-set plants grown in sterile substrate and later in unsterilized soil pots. The 16S rRNA analyses demonstrated high bacterial diversity with the majority closely related to B. megaterium followed by B. aryabhattai and B. cereus. B. pumilus, B. velezensis, B. safensis, B. flexus and Paenibacillus taichungensis species were also identified. Most strains were able to produce indol, solubilize zinc oxide and presented cellulase activity, while phosphate solubilization and siderophore production was observed in only few strains. The strains LMC21, LMC44 and LMC48 presented high biocontrol activity against the sugarcane fungi Colletotrichum falcatum. The spore formation was observed for strains grown for 8 days in the HCT medium but not in presence of sugarcane juice. Some strains also accumulated higher biomass in sugarcane (sterile conditions) and a mixture of three Bacillus strains performed better in the soil-pot inoculation experiment. These findings show for the first time that many Bacillus species are present in the apoplastic fluid of sugarcane grown in Brazil and indicate that some strains have PGP and biocontrol potential for application as biofertilizer in sugarcane plants. 653 $aEndophytes Taxonomy 653 $aMix inoculant 700 1 $aROCHA, F. Y. O. 700 1 $aVIDAL, M. S. 700 1 $aGITAHY, P. de M. 700 1 $aARRUDA, G. M. 700 1 $aBARRETO, C. P. 700 1 $aALVES, P. B. 700 1 $aRAMOS, E. T. de A. 773 $tApplied Soil Ecology, V. 166, 103985, 2021.
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