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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/07/1999 |
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Data da última atualização: |
21/07/1999 |
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Autoria: |
GOMES, P. C.; ROSTAGNO, H. S.; COSTA, P. M. de A.; PEREIRA, J. A. A.; LIMA, J. A. de F. |
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Afiliação: |
Embrapa Suinos e Aves, Concordia, SC. |
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Título: |
Digestibilidade aparente e verdadeira do fosforo de cinco alimentos determinadas com suinos em diferentes idades. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 23., 1986, Campo Grande, MS. Anais... Campo Grande: SBZ, 1986. |
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Páginas: |
p.44 |
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Idioma: |
Português |
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Thesagro: |
Digestibilidade; Fósforo; Suíno. |
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Thesaurus Nal: |
digestibility; phosphorus; swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00752naa a2200241 a 4500
001 1434130
005 1999-07-21
008 1986 bl uuuu u00u1 u #d
100 1 $aGOMES, P. C.
245 $aDigestibilidade aparente e verdadeira do fosforo de cinco alimentos determinadas com suinos em diferentes idades.
260 $c1986
300 $ap.44
650 $adigestibility
650 $aphosphorus
650 $aswine
650 $aDigestibilidade
650 $aFósforo
650 $aSuíno
700 1 $aROSTAGNO, H. S.
700 1 $aCOSTA, P. M. de A.
700 1 $aPEREIRA, J. A. A.
700 1 $aLIMA, J. A. de F.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 23., 1986, Campo Grande, MS. Anais... Campo Grande: SBZ, 1986.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
20/07/2021 |
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Data da última atualização: |
20/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MASCARIN, G. M.; IWANICKI, N. S.; RAMIREZ, J. L.; DELALIBERA JUNIOR, I.; DUNLAP, C. A. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; NATASHA SANT´ANNA IWANICKI, ESALQ-USP; JOSE LUIS RAMIREZ, USDA-ARS; ÍTALO DELALIBERA JUNIOR, ESALQ-USP. |
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Título: |
Transcriptional responses of Beauveria bassiana blastospores cultured under varying glucose concentrations. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Frontiers in Cellular and Infection Microbiology, v. 11, article 644372, 2021. |
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Páginas: |
p. 1-18. |
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ISSN: |
2235-2988 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications. MenosAbstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG ... Mostrar Tudo |
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Palavras-Chave: |
Biocontrol; Dimorphic growth; Liquid fermentation. |
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Thesagro: |
Beauveria Bassiana; Controle Biológico; Fermentação; Fungo Entomógeno; Fungo Para Controle Biológico. |
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Thesaurus NAL: |
Biological control; Cordycipitaceae; Liquid state fermentation; morphogenesis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224558/1/Mascarin-Transcriptional-responses-2021.pdf
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Marc: |
LEADER 02887naa a2200337 a 4500
001 2133044
005 2021-07-20
008 2021 bl uuuu u00u1 u #d
022 $a2235-2988
100 1 $aMASCARIN, G. M.
245 $aTranscriptional responses of Beauveria bassiana blastospores cultured under varying glucose concentrations.$h[electronic resource]
260 $c2021
300 $ap. 1-18.
520 $aAbstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications.
650 $aBiological control
650 $aCordycipitaceae
650 $aLiquid state fermentation
650 $amorphogenesis
650 $aBeauveria Bassiana
650 $aControle Biológico
650 $aFermentação
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
653 $aBiocontrol
653 $aDimorphic growth
653 $aLiquid fermentation
700 1 $aIWANICKI, N. S.
700 1 $aRAMIREZ, J. L.
700 1 $aDELALIBERA JUNIOR, I.
700 1 $aDUNLAP, C. A.
773 $tFrontiers in Cellular and Infection Microbiology$gv. 11, article 644372, 2021.
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Embrapa Meio Ambiente (CNPMA) |
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