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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/05/2019 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, T. B. dos; SOARES, J. D. M.; LIMA, J. E.; SILVA, J. C.; IVAMOTO, S. T.; BABA, V. Y.; SOUZA, S. G. H.; LORENZETTI, A. P. R.; PASCHOAL, A. R.; MEDA, A. R.; NISHIYAMA JÚNIOR, M. Y.; OLIVEIRA, U. C. de; MOKOCHINSKI, J. B.; GUYOT, R.; JUNQUEIRA-DE-AZEVEDO, I. L. M.; FIGUEIR, A. V. O.; MAZZAFERA, P.; R. JÚNIO, O.; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. |
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Afiliação: |
Tiago Benedito dos Santos, Universidade do Oeste Paulista; João D. M. Soares, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Joni E. Lima, Departamento de Botânica/Instituto de Ciências Biológicas/Universidade Federal de Minas Gerais - UFMG; Juliana C. Silva, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná; Suzana T. Ivamoto, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista; Viviane Y. Baba, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Silvia G. H. Souza, Laboratório de Biologia Molecular/Universidade Paranaense; Alan P. R. Lorenzetti, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL; Alexandre R. Paschoal, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná; Anderson R. Meda, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Milton Y. Nishiyama Júnior, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; Úrsula C. de Oliveira, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; João B. Mokochinski, Departamento de Biologia Vegetal, Instituto de Biologia/Universidade Estadual de Campinas; Romain Guyot, IRD, UMR IPME, COFFEEADAPT; Inácio L. M. Junqueira-de-Azevedo, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; Antônio V. O. Figueira, Centro de Energia Nuclear na Agricultura/Universidade de São Paulo - USP; Paulo Mazzafera, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL; Osvaldo R. Júnior, Life Sciences Core Facility - LaCTAD/Universidade Estadual de Campinas; Luiz G. E. Vieira, Universidade do Oeste Paulista; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; Douglas S. Domingues, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista. |
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Título: |
An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 19, n. 1, p. 151-169, January, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots. |
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Palavras-Chave: |
Differential gene expression; Nitrogen transport; RNA-seq. |
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Thesaurus Nal: |
MicroRNA. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196962/1/An-integrated-analysis-of-mRNA-transcriptional.pdf
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Marc: |
LEADER 02379naa a2200409 a 4500
001 2108713
005 2019-05-06
008 2019 bl uuuu u00u1 u #d
100 1 $aSANTOS, T. B. dos
245 $aAn integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots$binsights on nitrogen starvation responses.$h[electronic resource]
260 $c2019
520 $aCoffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots.
650 $aMicroRNA
653 $aDifferential gene expression
653 $aNitrogen transport
653 $aRNA-seq
700 1 $aSOARES, J. D. M.
700 1 $aLIMA, J. E.
700 1 $aSILVA, J. C.
700 1 $aIVAMOTO, S. T.
700 1 $aBABA, V. Y.
700 1 $aSOUZA, S. G. H.
700 1 $aLORENZETTI, A. P. R.
700 1 $aPASCHOAL, A. R.
700 1 $aMEDA, A. R.
700 1 $aNISHIYAMA JÚNIOR, M. Y.
700 1 $aOLIVEIRA, U. C. de
700 1 $aMOKOCHINSKI, J. B.
700 1 $aGUYOT, R.
700 1 $aJUNQUEIRA-DE-AZEVEDO, I. L. M.
700 1 $aFIGUEIR, A. V. O.
700 1 $aMAZZAFERA, P.
700 1 $aR. JÚNIO, O.
700 1 $aVIEIRA, L. G. E.
700 1 $aPEREIRA, L. F. P.
700 1 $aDOMINGUES, D. S.
773 $tFunctional & Integrative Genomics$gv. 19, n. 1, p. 151-169, January, 2019
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
22/07/2010 |
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Data da última atualização: |
19/04/2018 |
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Autoria: |
SILVA, M. H. M. da; RAKOCEVIC, M. |
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Afiliação: |
Murilo Hilário Mazza da Silva, Embrapa Informática Agropecuária; Miroslava Rakocevic, Instituto Agronômico do Paraná. |
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Título: |
Software for interpolation of vegetative growth of yerba mate plants in 3D. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 3, p. 244-251, mar. 2010 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Programa computacional para interpolação de crescimento vegetativo de plantas da erva-mate em 3D. |
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Conteúdo: |
Abstract ? The objective of this work was to build mock-ups of complete yerba mate plants in several stages of development, using the InterpolMate software, and to compute photosynthesis on the interpolated structure. The mock-ups of yerba-mate were first built in the VPlants software for three growth stages. Male and female plants grown in two contrasting environments (monoculture and forest understory) were considered. To model the dynamic 3D architecture of yerba-mate plants during the biennial growth interval between two subsequent prunings, data sets of branch development collected in 38 dates were used. The estimated values obtained from the mock-ups, including leaf photosynthesis and sexual dimorphism, are very close to those observed in the field. However, this similarity was limited to reconstructions that included growth units from original data sets. The modeling of growth dynamics enables the estimation of photosynthesis for the entire yerba mate plant, which is not easily measurable in the field. The InterpolMate software is efficient for building yerba mate mock-ups. |
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Palavras-Chave: |
3D reconstruction; AMAPmod; Arquitetura de planta; Crescimento de planta; Modelagem; Modeling; Reconstrução 3D. |
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Thesagro: |
Ilex Paraguariensis. |
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Thesaurus NAL: |
Models; Plant architecture; Plant growth. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/48043/1/45n03a03.pdf
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Marc: |
LEADER 01996naa a2200277 a 4500
001 1858423
005 2018-04-19
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, M. H. M. da
245 $aSoftware for interpolation of vegetative growth of yerba mate plants in 3D.
260 $c2010
500 $aTítulo em português: Programa computacional para interpolação de crescimento vegetativo de plantas da erva-mate em 3D.
520 $aAbstract ? The objective of this work was to build mock-ups of complete yerba mate plants in several stages of development, using the InterpolMate software, and to compute photosynthesis on the interpolated structure. The mock-ups of yerba-mate were first built in the VPlants software for three growth stages. Male and female plants grown in two contrasting environments (monoculture and forest understory) were considered. To model the dynamic 3D architecture of yerba-mate plants during the biennial growth interval between two subsequent prunings, data sets of branch development collected in 38 dates were used. The estimated values obtained from the mock-ups, including leaf photosynthesis and sexual dimorphism, are very close to those observed in the field. However, this similarity was limited to reconstructions that included growth units from original data sets. The modeling of growth dynamics enables the estimation of photosynthesis for the entire yerba mate plant, which is not easily measurable in the field. The InterpolMate software is efficient for building yerba mate mock-ups.
650 $aModels
650 $aPlant architecture
650 $aPlant growth
650 $aIlex Paraguariensis
653 $a3D reconstruction
653 $aAMAPmod
653 $aArquitetura de planta
653 $aCrescimento de planta
653 $aModelagem
653 $aModeling
653 $aReconstrução 3D
700 1 $aRAKOCEVIC, M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 45, n. 3, p. 244-251, mar. 2010
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Embrapa Unidades Centrais (AI-SEDE) |
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