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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
01/12/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOTA, F. F. da; GOMES, E. A.; SELDIN, L. |
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Afiliação: |
Fabio Faria da Mota, UFRJ; ELIANE APARECIDA GOMES, CNPMS; Lucy Seldin, UFRJ. |
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Título: |
Auxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Journal of Microbiology, v. 46, n. 3, p. 257-264, 2008. |
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DOI: |
10.1007/s12275-007-0245-x |
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Idioma: |
Inglês |
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Conteúdo: |
Different species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome. MenosDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not pre... Mostrar Tudo |
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Palavras-Chave: |
Auxin; Auxin efflux earrier protein; Indole-3-aeetic acid; Paenibacillus graminis. |
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Thesaurus Nal: |
Paenibacillus polymyxa. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02470naa a2200217 a 4500
001 1491541
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1007/s12275-007-0245-x$2DOI
100 1 $aMOTA, F. F. da
245 $aAuxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa.$h[electronic resource]
260 $c2008
520 $aDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome.
650 $aPaenibacillus polymyxa
653 $aAuxin
653 $aAuxin efflux earrier protein
653 $aIndole-3-aeetic acid
653 $aPaenibacillus graminis
700 1 $aGOMES, E. A.
700 1 $aSELDIN, L.
773 $tJournal of Microbiology$gv. 46, n. 3, p. 257-264, 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Classificação |
Cutter |
Registro |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
28/07/2015 |
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Data da última atualização: |
19/08/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FONSECA, D.; FERRACINI, V. L.; QUEIROZ, S. C. do N. de; TRAPE, A. Z. |
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Afiliação: |
DELBA FONSECA; VERA LUCIA FERRACINI, CNPMA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; ANGELO A. TRAPE. |
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Título: |
Monitoring of N-Methylcarbamates and some of metabolites in water the lake the Furnas, Minas Gerais, Brazil. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PESQUISAS AMBIENTAIS E SAÚDE, 5., 2005, Santos/SP. Anais... Santos/SP: CBPAS, 2005. p.218-221. |
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Idioma: |
Inglês |
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Conteúdo: |
The Lake Of Furnas, The Largest Manmade In Brazil, Is Officiallly Designated By The State Of Minas Gerais As And Important Role Economic, Supply Energy Electric Long Of The To Attend Activity Fishing And Water Has Been At Source Irrigation Annual The Potato, Corn, Bean, Coffee And Sugarcane Intensive Agricuture. The Purpose Of This Study Was To Determine Aldicarb, Aldicarb Sulfone, Carbaryl, Carbofuran, 3-Hydroxycarbofuran And Methomyl In Surface Water. Water Samples Were Taken For The Period From October 2003 To March 2004 To Describe And Compare The Occurrence And Distribution Of Pesticides In Agricultural Area. Water Samples Were Most Frequently Contaminated By Carbofuran And 3-Hidroxycarbofuran Compounds, Carbaryl, Methomyl , Aldicarb And Aldicarb Sulfone, Although In Low (0.1 ?gl-1) Concentrations. However, The January Through March Samples Exhibited Of All Pesticides And Metabolites, With Concentrations To 0.10 To 120.00 Ngl-1. These Results Underscore The Need To Improved Environmental Protection Measures. |
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Palavras-Chave: |
Contaminação ambiental; Metilcarbamates; Monitoramento ambiental. |
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Thesagro: |
Água; Contaminação. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127069/1/2005AA-040.pdf
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Marc: |
LEADER 01757nam a2200205 a 4500
001 2020669
005 2015-08-19
008 2005 bl uuuu u00u1 u #d
100 1 $aFONSECA, D.
245 $aMonitoring of N-Methylcarbamates and some of metabolites in water the lake the Furnas, Minas Gerais, Brazil.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE PESQUISAS AMBIENTAIS E SAÚDE, 5., 2005, Santos/SP. Anais... Santos/SP: CBPAS, 2005. p.218-221.$c2005
520 $aThe Lake Of Furnas, The Largest Manmade In Brazil, Is Officiallly Designated By The State Of Minas Gerais As And Important Role Economic, Supply Energy Electric Long Of The To Attend Activity Fishing And Water Has Been At Source Irrigation Annual The Potato, Corn, Bean, Coffee And Sugarcane Intensive Agricuture. The Purpose Of This Study Was To Determine Aldicarb, Aldicarb Sulfone, Carbaryl, Carbofuran, 3-Hydroxycarbofuran And Methomyl In Surface Water. Water Samples Were Taken For The Period From October 2003 To March 2004 To Describe And Compare The Occurrence And Distribution Of Pesticides In Agricultural Area. Water Samples Were Most Frequently Contaminated By Carbofuran And 3-Hidroxycarbofuran Compounds, Carbaryl, Methomyl , Aldicarb And Aldicarb Sulfone, Although In Low (0.1 ?gl-1) Concentrations. However, The January Through March Samples Exhibited Of All Pesticides And Metabolites, With Concentrations To 0.10 To 120.00 Ngl-1. These Results Underscore The Need To Improved Environmental Protection Measures.
650 $aÁgua
650 $aContaminação
653 $aContaminação ambiental
653 $aMetilcarbamates
653 $aMonitoramento ambiental
700 1 $aFERRACINI, V. L.
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aTRAPE, A. Z.
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