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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/12/2016 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS-JUNIOR, P. H. A.; ALVES, T. J. M.; DIAS, M. T.; ASSUNÇAO, C. M.; MUNK, M.; MATTOS, M. S.; KRAEMER, L. R.; ALMEIDA, B. G.; RUSSO, R. C.; BARCELOS, L.; CAMARGO, L. S. de A.; VIANA, J. H. M. |
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Afiliação: |
PAULO HENRIQUE ALMEIDA CAMPOS-JUNIOR, UFSJ; THALYS JAIR MELO ALVES, UFSJ; MARCO TULIO DIAS, UFSJ; CAROLINA MARINHO ASSUNÇAO, UFJF; MICHELE MUNK, UFJF; MATHEUS SILVÉRIO MATTOS, UFMG; LUCAS ROCHA KRAEMER, UFMG; BRÍGIDA GOMES ALMEIDA, UFMG; REMO CASTRO RUSSO, UFMG; LUCÍOLA BARCELOS, UFMG; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Ovarian Grafts 10 days after Xenotransplantation: folliculogenesis and recovery of viable oocytes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 6, e0158109, 2016. |
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DOI: |
10.1371/journal. pone.0158109 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes. MenosAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. H... Mostrar Tudo |
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Palavras-Chave: |
Bovine ovarian grafts; Ovarian xenografts. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154925/1/Cnpgl-2016-PlosOne-Ovarian.PDF
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Marc: |
LEADER 02628naa a2200301 a 4500
001 2062986
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1371/journal. pone.0158109$2DOI
100 1 $aCAMPOS-JUNIOR, P. H. A.
245 $aOvarian Grafts 10 days after Xenotransplantation$bfolliculogenesis and recovery of viable oocytes.$h[electronic resource]
260 $c2016
520 $aAbstracts Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.
650 $aoocytes
653 $aBovine ovarian grafts
653 $aOvarian xenografts
700 1 $aALVES, T. J. M.
700 1 $aDIAS, M. T.
700 1 $aASSUNÇAO, C. M.
700 1 $aMUNK, M.
700 1 $aMATTOS, M. S.
700 1 $aKRAEMER, L. R.
700 1 $aALMEIDA, B. G.
700 1 $aRUSSO, R. C.
700 1 $aBARCELOS, L.
700 1 $aCAMARGO, L. S. de A.
700 1 $aVIANA, J. H. M.
773 $tPlos One$gv. 11, n. 6, e0158109, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
15/06/1999 |
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Data da última atualização: |
03/09/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ORTIZ PENA, C. D.; CAMPOS, F. P. de; FEITOSA, J. V.; QUEIROZ, S. A. de; ALENCAR, M. M. de; ROCHA, J. C. M. C.; SIQUEIRA, R. P. G.; HELLMEISTER FILHO, P.; ROCHA, C. E. |
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Afiliação: |
CARLOS DARIO O. PEÑA, UNESP; FABIO PRUDÊNCIO DE CAMPOS, UNESP; JOSÉ VALMIR FEITOSA, UNESP; SANDRA AIDAR DE QUEIROZ, UNESP; MAURICIO MELLO DE ALENCAR, CPPSE; JOSYANNE COSTA M. C. ROCHA, UNESP; ROBERTA PONTES G. SIQUEIRA, UNESP; PAULO HELLMEISTER FILHO, UNESP; CARLOS EDUARDO ROCHA, UNESP. |
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Título: |
Estimativas de herdabilidade do perímetro escrotal, peso corporal e suas relações em tourinhos da raça Nelore no Paraguai. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNICA, 35., 1998, Botucatu, SP. Anais...Botucatu: SBZ, 1998. p.365-367. |
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Idioma: |
Português |
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Notas: |
Resumo expandido. |
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Conteúdo: |
O objetivo deste trabalho foi estimar componentes de variância e coeficiente de herdabilidade do peso ajustado aos 570 dias (P570), perímetro escrotal(PE), PE ajustado para idade do animal (PEi), ajustado somente para peso (PEp), e para idade e peso (PEip), PE padronizado pelo desvio padrão do grupo contemporâneo (PEs), PEs ajustado para idade (PEsi), ajustado somente para peso (PEsp), e ajustado para idade e peso (PEsip), da relação PE/peso ao sobreano (IND), e IND ajustado para idade (INDi), em 1250 tourinhos de um rebanho da raça Nelore no Paraguai, nascidos entre 1986 e 1993. |
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Palavras-Chave: |
Adjusted live weight; age deffect; Efeito de idade; Peso ajustado; Peso sobreano; yearling weight. |
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Thesagro: |
Gado de Corte. |
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Thesaurus NAL: |
beef cattle. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/12334/1/PROCIMMA1998.00071.pdf
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Marc: |
LEADER 01621nam a2200313 a 4500
001 1044391
005 2021-09-03
008 1998 bl uuuu u00u1 u #d
100 1 $aORTIZ PENA, C. D.
245 $aEstimativas de herdabilidade do perímetro escrotal, peso corporal e suas relações em tourinhos da raça Nelore no Paraguai.$h[electronic resource]
260 $aIn: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNICA, 35., 1998, Botucatu, SP. Anais...Botucatu: SBZ, 1998. p.365-367.$c1998
500 $aResumo expandido.
520 $aO objetivo deste trabalho foi estimar componentes de variância e coeficiente de herdabilidade do peso ajustado aos 570 dias (P570), perímetro escrotal(PE), PE ajustado para idade do animal (PEi), ajustado somente para peso (PEp), e para idade e peso (PEip), PE padronizado pelo desvio padrão do grupo contemporâneo (PEs), PEs ajustado para idade (PEsi), ajustado somente para peso (PEsp), e ajustado para idade e peso (PEsip), da relação PE/peso ao sobreano (IND), e IND ajustado para idade (INDi), em 1250 tourinhos de um rebanho da raça Nelore no Paraguai, nascidos entre 1986 e 1993.
650 $abeef cattle
650 $aGado de Corte
653 $aAdjusted live weight
653 $aage deffect
653 $aEfeito de idade
653 $aPeso ajustado
653 $aPeso sobreano
653 $ayearling weight
700 1 $aCAMPOS, F. P. de
700 1 $aFEITOSA, J. V.
700 1 $aQUEIROZ, S. A. de
700 1 $aALENCAR, M. M. de
700 1 $aROCHA, J. C. M. C.
700 1 $aSIQUEIRA, R. P. G.
700 1 $aHELLMEISTER FILHO, P.
700 1 $aROCHA, C. E.
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Embrapa Pecuária Sudeste (CPPSE) |
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