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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
15/09/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. |
Afiliação: |
ISIS VIANA MENDES, Universidade de Brasília; MARIANA BOTELHO GARCIA, Universidade Católica de Brasília; ANA CAROLINA ARAUJO BITENCOURT, Universidade de Brasília; RENATA HENRIQUE SANTANA, Instituto Federal de Brasília; PHILIPPE DE CASTRO LINS, Universidade de Brasília; RAFAELLA SILVEIRA, Universidade de Brasília; BLAKE A. SIMMONS, Joint BioEnergy Institute; JOHN M. GLADDEN, Joint BioEnergy Institute; RICARDO HENRIQUE KRUGER, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
Título: |
Bacterial diversity dynamics in microbial consortia selected for lignin utilization. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
PLoS ONE, v. 16, n. 9, e0255083, 2021. |
Descrição Física: |
PDF: il. color. |
DOI: |
https://doi.org/10.1371/journal.pone.0255083 |
Idioma: |
Inglês |
Conteúdo: |
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. MenosAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lign... Mostrar Tudo |
Thesagro: |
Biotecnologia; Carbono; Composto Fenólico; Lignina. |
Thesaurus Nal: |
Application technology; Biotechnology; Carbon; Degradation; Energy conversion; Lignin; Phenolic compounds. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226045/1/Bacterial-diversity-dynamics-in-microbial-2021.pdf
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Marc: |
LEADER 03127naa a2200385 a 4500 001 2134421 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal.pone.0255083$2DOI 100 1 $aMENDES, I. V. 245 $aBacterial diversity dynamics in microbial consortia selected for lignin utilization.$h[electronic resource] 260 $c2021 300 $cPDF: il. color. 520 $aAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. 650 $aApplication technology 650 $aBiotechnology 650 $aCarbon 650 $aDegradation 650 $aEnergy conversion 650 $aLignin 650 $aPhenolic compounds 650 $aBiotecnologia 650 $aCarbono 650 $aComposto Fenólico 650 $aLignina 700 1 $aGARCIA, M. B. 700 1 $aBITENCOURT, A. C. A. 700 1 $aSANTANA, R. H. 700 1 $aLINS, P. de C. 700 1 $aSILVEIRA, R. 700 1 $aSIMMONS, B. A. 700 1 $aGLADDEN, J. M. 700 1 $aKRUGER, R. H. 700 1 $aQUIRINO, B. F. 773 $tPLoS ONE$gv. 16, n. 9, e0255083, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
27/08/2015 |
Data da última atualização: |
29/02/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FIGUEIREDO, E. S. de; QUEIROZ, D. L. de; BURCKHARDT, D. H.; GOMES, F. B.; SOUSA, A. C. P. de; KRUG, C. |
Afiliação: |
Elias Soares de Figueiredo, Bolsista; DALVA LUIZ DE QUEIROZ, CNPF; Daniel Hieronymus Burckhardt, Naturhistorisches Museum Basel; FLAVIA BATISTA GOMES, CPAA; Ana Cláudia Pereira de Sousa, Bolsista; CRISTIANE KRUG, CPAA. |
Título: |
Ocorrência de Psilídeos em plantios florestais experimentais e em vegetação nativa. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 11., 2014, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 19-20. |
Idioma: |
Português |
Conteúdo: |
O objetivo deste trabalho foi monitorar e identificar os psilídeos em 15 plantios florestais experimentais e na vegetação nativa no Campo Experimental da Embrapa Amazônia Ocidental em Manaus, AM. |
Palavras-Chave: |
Angelim-pedra; Caroba; Freijó; Louro inhamui; Ocotea sp; Potenciais insetos-praga; Schefflera morototoni; Taxi-branco; Virola sebifera. |
Thesagro: |
Acácia; Andiroba; Bertholletia Excelsa; Biodiversidade; Carapa Guianensis; Castanha; Cedrelinga Catenaeformis; Cedrorana; Cordia Goeldiana; Cumaru; Cupiúba; Dipteryx Odorata; Goupia Glabra; Hymenaea Courbaril; Hymenolobium Petraeum; Jatobá; Morototó; Parkia Multijuga; Sclerolobium Paniculatum; Ucuuba. |
Thesaurus NAL: |
Acacia mangium; Amazonia; Jacaranda copaia; Parkia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140289/1/XI-Jornada-IC-Psilideos.pdf
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Marc: |
LEADER 01811nam a2200565 a 4500 001 2022815 005 2016-02-29 008 2015 bl uuuu u00u1 u #d 100 1 $aFIGUEIREDO, E. S. de 245 $aOcorrência de Psilídeos em plantios florestais experimentais e em vegetação nativa.$h[electronic resource] 260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 11., 2014, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 19-20.$c2015 520 $aO objetivo deste trabalho foi monitorar e identificar os psilídeos em 15 plantios florestais experimentais e na vegetação nativa no Campo Experimental da Embrapa Amazônia Ocidental em Manaus, AM. 650 $aAcacia mangium 650 $aAmazonia 650 $aJacaranda copaia 650 $aParkia 650 $aAcácia 650 $aAndiroba 650 $aBertholletia Excelsa 650 $aBiodiversidade 650 $aCarapa Guianensis 650 $aCastanha 650 $aCedrelinga Catenaeformis 650 $aCedrorana 650 $aCordia Goeldiana 650 $aCumaru 650 $aCupiúba 650 $aDipteryx Odorata 650 $aGoupia Glabra 650 $aHymenaea Courbaril 650 $aHymenolobium Petraeum 650 $aJatobá 650 $aMorototó 650 $aParkia Multijuga 650 $aSclerolobium Paniculatum 650 $aUcuuba 653 $aAngelim-pedra 653 $aCaroba 653 $aFreijó 653 $aLouro inhamui 653 $aOcotea sp 653 $aPotenciais insetos-praga 653 $aSchefflera morototoni 653 $aTaxi-branco 653 $aVirola sebifera 700 1 $aQUEIROZ, D. L. de 700 1 $aBURCKHARDT, D. H. 700 1 $aGOMES, F. B. 700 1 $aSOUSA, A. C. P. de 700 1 $aKRUG, C.
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