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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
22/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. |
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Afiliação: |
Felipe Perecin, USP/Pirassununga; SIMONE CRISTINA MEO NICIURA, CPPSE; Walt Yamazaki, Biotecnologia da Reprodução Animal Ltda; Christina Ramires Ferreira, Unicamp; Fernando Henrique Biase, USP/Pirassununga; Giovana Krentel Merighe, USP/Pirassununga; Flávio V. Meirelles, USP/Pirassununga; Joaquim Mansano Garcia, UNESP/Jaboticabal. |
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Título: |
Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Some gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers. MenosSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed rea... Mostrar Tudo |
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Palavras-Chave: |
In vivo-and in vitro; Placentas; Produced bovine. |
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Thesaurus Nal: |
gene expression. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42088/1/PROCI-2008.00004.pdf
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Marc: |
LEADER 03515nam a2200241 a 4500
001 1048224
005 2023-06-22
008 2008 bl uuuu u00u1 u #d
100 1 $aPERECIN, F.
245 $aImprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 20, n. 1, p. 173$c2008
520 $aSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers.
650 $agene expression
653 $aIn vivo-and in vitro
653 $aPlacentas
653 $aProduced bovine
700 1 $aNICIURA, S. C. M.
700 1 $aYAMAZAKI, W.
700 1 $aFERREIRA, C. R.
700 1 $aBIASE, F. H.
700 1 $aMERIGHE, G. K. F.
700 1 $aMEIRELLES, F. V.
700 1 $aGARCIA, J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 208 | |
| 61. |  | PETTER, F. A.; PACHECO, L. P.; PROCOPIO, S. de O.; CARGNELUTTI FILHO, A.; VOLF, M. R. Seletividade de herbicidas à cultura do milho e ao capim-braquiária cultivadas no sistema de integração lavoura-pecuária. Semina. Ciências Agrárias, Londrina, v. 32, n. 3, p. 855-864, jul/set. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
|    |
| 66. | | PIRES, F. R.; PROCÓPIO, S. de O.; SANTOS, J. B. dos; SOUZA, C. M. de; DIAS, R. R. Avaliação da fitorremediação de tebuthiuron utilizando crotalaria juncea como planta indicadora. Revista Ciência Agronômica, Fortaleza, v. 39, n. 2, p. 245-250, abr./jul. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
|  |
| 68. | | FONTES, H. R.; PROCOPIO, S. de O.; CARGNELUTTI FILHO, A.; FERREIRA, J. M. S.; FERNANDES, M. F. Avaliação de diferentes herbicidas para erradicação química de coqueiros infectados com resinose. Planta Daninha, Viçosa, v. 27, n.4, p. 849-857, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 69. | | CINTRA, F. L. D.; RESENDE, R. S.; PROCOPIO, S. de O.; PACHECO, E. P.; FONTES, H. R. Cobertura morta com palha de coqueiro e biomanta: estratégias para redução do volume de água de irrigação em coqueirais. In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3.; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS. 3., 2004, Brasília, DF; Aracaju, SE. Seifrut: [anais..]. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. 197 p. Editores Técnicos: Carlos Roberto Martins, Embrapa Clima Temperado; Francisco Alisson Xavier, Embrapa Mandioca; Antônio Souza do Nascimento, Embrapa Mandioca e Fruticultura.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 73. | | PETTER, F. A.; PROCÓPIO, S. de O.; CARGNELUTTI FILHO, A.; BARROSO, A. L. de L.; PACHECO, L. P. Posicionamento de herbicidas na cultura da soja Roundup ready. In: CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 26.; CONGRESSO DE LA ASSOCIACIÓN LATINO-AMERICANA DE MALEZAS, 18., 2008, Ouro Preto. Trabalhos... Sete Lagoas: SBCPD : Embrapa Milho e Sorgo, 2008. 1 CD-ROM. pdf n. 32.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 74. | | BUCHLING, C; BRAZ, G. B. P.; PROCOPIO, S. de O.; FERREIRA, C. J. B.; SILVA, A. G. da; CORADIN, J. Pre-emergence control and interference of voluntary maize plants on a soybean crop in Brazilian Cerrado. Acta Scientiarum. Agronomy, v. 44, n. 1, e54544, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
|  |
| 77. | | MADALAO, J. C.; PIRES, F. R.; NASCIMENTO, A. F.; CHAGAS, K.; CARGNELUTTI FILHO, A.; PROCOPIO, S. de O. Fitorremediação de solo contaminado com sulfentrazone em função do tempo de cultivo de Canavalia ensiformis. Agro@mbiente, Boa Vista, v. 10, n. 1, p. 36-43, jan./mar. 2016. On-line.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 79. | | FERNANDES, M. F.; PROCOPIO, S. de O.; TELES, D. A.; CARGNELUTTI FILHO, A.; SANTANA, S. A. C. de. Efeitos de inseticidas utilizados na cultura da cana-de-açúcar sobre parâmetros de crescimento da bactéria diazotrófica Azospirillum brasilense. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 80. | | FERNANDES, M. F.; PROCOPIO, S. de O.; TELES, D. A.; CARGNELUTTI FILHO, A.; SANTANA, S. A. C. de. Efeitos de inseticidas utilizados na cultura da cana-de-açúcar sobre parâmetros de crescimento da bactéria diazotrófica Gluconacetobacter diazotrophicus. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. 1 CD-ROM. FERTBIO 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 208 | |
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