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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
23/10/2009 |
Data da última atualização: |
01/03/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANDREOTTI, R.; MATOS, M. de F. C.; GONÇALVES, K. N.; OSHIRO, L. M.; LIMA-JUNIOR, M. S. da C.; PAIVA, F.; LEITE, F. L. |
Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; MARIA DE FÁTIMA CEPA MATOS, UFMS; KELLY NODA GONÇALVES, UFMS; LEANDRA MARLA OSHIRO, UFMS; MANOEL SEBASTIÃO DA COSTA LIMA-JUNIOR, UFMS; FERNANDO PAIVA, UFMS; FÁBIO LEIVAS LEITE, UFPel. |
Título: |
Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, São Paulo, v.18, n.2, p.20-24, abr-jun. 2009. |
Idioma: |
Inglês |
Conteúdo: |
Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG
tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep. |
Palavras-Chave: |
NcSRS2; Proteína recombinante; Teste. |
Thesagro: |
Diagnostico; Elisa; Neospora Caninum; Neosporose; Ovino; Sanidade Animal. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02075naa a2200301 a 4500 001 1573041 005 2010-03-01 008 2009 bl --- 0-- u #d 100 1 $aANDREOTTI, R. 245 $aComparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep. 260 $c2009 520 $aNeospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep. 650 $aDiagnostico 650 $aElisa 650 $aNeospora Caninum 650 $aNeosporose 650 $aOvino 650 $aSanidade Animal 653 $aNcSRS2 653 $aProteína recombinante 653 $aTeste 700 1 $aMATOS, M. de F. C. 700 1 $aGONÇALVES, K. N. 700 1 $aOSHIRO, L. M. 700 1 $aLIMA-JUNIOR, M. S. da C. 700 1 $aPAIVA, F. 700 1 $aLEITE, F. L. 773 $tRevista Brasileira de Parasitologia Veterinária, São Paulo$gv.18, n.2, p.20-24, abr-jun. 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
17/09/2019 |
Data da última atualização: |
06/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUZA, B. B. P.; CARDOZO FILHO, J. L.; MURAD, A. M.; PRATES, M. V.; COURA, M. M. A.; BRAND, G. D.; BARBOSA, E. A.; BLOCH JUNIOR, C. |
Afiliação: |
BEATRIZ BLENDA P. SOUZA, UNB; JOSÉ L. CARDOZO FILHO, INSTITUTO MATO-GROSSENSE DO ALGODÃO; ANDRE MELRO MURAD, Cenargen; MAURA VIANNA PRATES, Cenargen; MARCELO M. A. COURA, UNB; GUILHERME D. BRAND, UNB; EDER A. BARBOSA, UNB; CARLOS BLOCH JUNIOR, Cenargen. |
Título: |
Identification and characterization of phospholipases A2 from the skin secretion of Pithecopus azureus anuran. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Toxicon, v. 167, p. 10-19, 2019. |
DOI: |
10.1016/j.toxicon.2019.06.002 |
Idioma: |
Inglês |
Notas: |
Na publicação: José L. Cardozo Fh.; Carlos Bloch Jr. |
Palavras-Chave: |
Amino acid and cDNA sequencing; Molecular modeling; N-Glycosylation; Pithecopus azureus. |
Thesaurus NAL: |
Phospholipase A2. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00900naa a2200277 a 4500 001 2112238 005 2020-04-06 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.toxicon.2019.06.002$2DOI 100 1 $aSOUZA, B. B. P. 245 $aIdentification and characterization of phospholipases A2 from the skin secretion of Pithecopus azureus anuran.$h[electronic resource] 260 $c2019 500 $aNa publicação: José L. Cardozo Fh.; Carlos Bloch Jr. 650 $aPhospholipase A2 653 $aAmino acid and cDNA sequencing 653 $aMolecular modeling 653 $aN-Glycosylation 653 $aPithecopus azureus 700 1 $aCARDOZO FILHO, J. L. 700 1 $aMURAD, A. M. 700 1 $aPRATES, M. V. 700 1 $aCOURA, M. M. A. 700 1 $aBRAND, G. D. 700 1 $aBARBOSA, E. A. 700 1 $aBLOCH JUNIOR, C. 773 $tToxicon$gv. 167, p. 10-19, 2019.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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