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| Registros recuperados : 10 | |
| 4. |  | MORANDI, M. A. B.; POMELLA, A. W. V.; SANTOS, E. R. dos; FERNANDES, M.; CAOVILA, L. E.; FERNANDES, A. O. Selection of Trichoderma SPP. isolates to control the bean white-mold fungus Sclerotinia clerotiorum in winter crops. In: MEETING OF THE 'PHYTOPATHOGENS' GROUP: FUNDAMENTAL AND PRACTICAL APPROACHES TO INCREASE BIOCONTROL EFFICACY, 9., 2006, Belgium. Abstracts... Belgium: IOBC/WPRS, 2006.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 5. | | MORANDI, M. A. B.; POMELLA, A. W. V.; SANTOS, E. R. dos; FERNANDES, M.; CAOVILA, L. E.; FERNANDES, A. O. Selection of Trichoderma spp. isolates to control the bean white-mold fungus Sclerotinia sclerotiorum in winter crops. In: MEETING OF THE WORKING GROUP "BIOLOGICAL CONTROL OF FUNGAL AND BACTERIAL PLANT PATHOGENS", 9., 2006, Spa, Belgium. Proceedings... IOBC/WPRS Bulletin, v.30, n.6, p.79-82, 2007.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | BALIGAR, V. C.; FAGERIA, N. K.; PAIVA, A. Q.; SILVEIRA, A.; POMELLA, A. W. V.; MACHADO, R. C. R. Light intensity effects on growth and micronutrient uptake by tropical legume cover crops. Journal of Plant Nutrition, v. 29, n. 11, p. 1959-1974, Nov. 2006.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. Toxins, v. 6, p. 1598-1614, 2014.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | BALIGAR, V. C.; FAGERIA, N. K.; PAIVA, A.; SILVEIRA, A.; SOUZA JUNIOR, J. O. de; LUCENA, E.; FARIA, J. C.; CABRAL, R.; POMELLA, A. W. V.; JORDA JUNIOR, J. Light intensity effects on growth and nutrient-use efficiency of tropical legume cover crops. In: JOSE, S.; GORDON, A. M. (Ed.). Toward agroforestry design: an ecological approach. Dordrecht: Springer, 2008. p. 67-79. (Advances in agroforestry, 4).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | SANTOS, C. M. B.; COELHO, G. M.; CUPERTINO, F. B.; PIOVANI, C. P. P.; CASCARDO, J. C. M.; LOGUERCIO, L. L.; FÉLIX, C. R.; DE MARCO, J. L.; CARVALHO, L. J. C. B.; POMELLA, A. W. V.; COSTA, M. G. C. Proteômica de fungos filamentosos: mapeamento inicial do proteoma extracelular do agente de controle biológico Trichoderma stromaticum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 1082.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 10 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
29/05/2014 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Toxins, v. 6, p. 1598-1614, 2014. |
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DOI: |
10.3390/toxins6051598 |
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Idioma: |
Inglês |
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Conteúdo: |
Bioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. MenosBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activ... Mostrar Tudo |
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Thesagro: |
Toxina. |
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Categoria do assunto: |
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Marc: |
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024 7 $a10.3390/toxins6051598$2DOI
100 1 $aARGÔLO FILHO, R. C.
245 $aRequirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.$h[electronic resource]
260 $c2014
520 $aBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems.
650 $aToxina
700 1 $aCOSTA, R. L.
700 1 $aPINHEIRO, H. E.
700 1 $aCORRÊA, F. M.
700 1 $aVALICENTE, F. H.
700 1 $aPOMELLA, A. W. V.
700 1 $aLOGUERCIO, L. L.
773 $tToxins$gv. 6, p. 1598-1614, 2014.
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