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| Registros recuperados : 42 | |
| 6. |  | MILANEZ, A. M.; PRATISSOLI, D.; POLANCZYK, R. A.; BUENO, A. de F.; TUFIK, C. B. A. Avaliação de Trichogramma spp. para o controle de Trichoplusia ni. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1219-1224, out. 2009.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Soja; Embrapa Unidades Centrais. |
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| 7. | | PRATISSOLI, D.; POLANCZYK, R. A.; GRECCO, E. D.; FERREIRA, R. A.; HOLTZ, A. M. Efeito entomotóxico de novos isolados de Bacillus thuringiensis em duas populações de Spodoptera frugiperda oriundas de Minas Gerais e do Espirito Santo. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 6, n. 2, p. 140-148, 2007.| Biblioteca(s): Embrapa Algodão; Embrapa Milho e Sorgo. |
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| 9. | | BORGES FILHO, R. da C.; ZANARDI, O. Z.; NAVA, D. E.; POLANCZYK, R. A.; PRATISSOLI, D. Desenvolvimento ninfal de Pachycoris torridus (Scopoli, 1772) (Hemiptera: scutelleridae) em diferentes hospedeiros. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | PRATISSOLI, D.; POLANCZYK, R. A.; HOLTZ, A. M.; COCHETO, J. G.; TAMANHONI, T.; MILANEZ, A. M. Desenvolvimento da broca-das-cucurbitáceas em diferentes tipos de substratos alimentares. Horticultura Brasileira, Brasília, DF, v. 25, n. 4, p. 598-601, out./dez. 2007.| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | PRATISSOLI, D.; POLANCZYK, R. A.; DALVI, L. P.; COCHETO, J. G.; MELO, D. F. Ocorrência de Ascia monuste orseis (Lepidoptera: Pieridae) danificando mudas de Crataeva tapia. Ciência Rural, Santa Maria, RS, v. 37, n. 3, p. 874-875, maio/jun. 2007. Nota.| Biblioteca(s): Embrapa Florestas. |
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| 13. | | MELO, D. F.; PRATISSOLI, D.; NAVA, D. E.; POLANCZYK, R. A.; STINGUEL, P.; KLOSS, T. G.; ALVES, F. C. Coleta e identificação de espécies e ou linhagens de Trichogramma (Hymenoptera: Trichogrammatidae) que ocorrem em fruteiras no Rio Grande do Sul. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. resumos... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | PRATISSOLI, D.; POLANCZYK, R. A.; ANDRADE, G. S.; HOLTZ, A. M.; SILVA, A. F. da; PASTORI, P. L. Tabela de vida de fertilidade de cinco linhagens de Trichogramma pretiosum Riley (Hym.: Trichogrammatidae) criadas em ovos de Tuta absoluta (Merick) (Lep.: Gelechiidae), sob temperaturas constantes e alternadas. Ciência Rural, Santa Maria, v. 37, n. 3, p. 618-622, 2007.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 15. | | PRATISSOLI, D.; POLANCZYK, R. A.; HOLTZ, A. M.; DALVI, L. P.; SILVA, A. F.; SILVA, L. N. Selection of Trichogramma species for controlling the Diamondback moth. Horticultura Brasileira, Brasília, DF, v. 26, n. 2, p. 194-196, jun. 2008.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | PRATISSOLI, D.; POLANCZYK, R. A.; PEREIRA, C. L. T.; FURTADO, I. S. de A.; COCHETO, J. G. Influência da fase embrionária dos ovos da traça-das-crucíferas sobre fêmeas de Trichogramma pretiosum com diferentes idades. Horticultura Brasileira, Brasília, DF, v. 25, n. 2, p. 286-290, abr./jun. 2007.| Biblioteca(s): Embrapa Hortaliças. |
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| 17. | | SILVA, I H. S. da; COSTA, V. H. D. da; CURY, J. de C.; VALICENTE, F. H.; POLANCZYK, R. A. The infectivity of SfMNPV on fall armyworm is influenced by the host plant. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 369-379, 2018.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | FILHO, R. C. B.; PRASTISSOLI, D.; NAVA, D. E.; MONTE, F. G.; GUIDONI, A. L.; POLANCZYK, R. A. Biologia de Pachycoris torridus (Hemiptera: Scutelleridae) em diferentes hospedeiros. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012.| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | BORGES FILHO, R. da C.; NAVA, D. E.; PRATISSOLI, D.; POLANCZYK, R. A.; MARANGON, R. B.; LOIÁCONO, M. Biology of Telenomus pachycoris (Hymenoptera: Scelionidae), a parasitoid of eggs of Pachycoris torridus (Hemiptera: Scutelleridae): the effects of egg age, exposure time, and temperature. Florida Entomologist, v. 100, n. 2, p. 375-379, 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 20. | | PRATISSOLI, D.; DALVI, L. P.; POLANCZYK, R. A.; ANDRADE, G. S.; SILVA, A. F. da; BELINELO, V. J. Efeito do óleo de nim (Azadirachta indica) sobre ovos de Helicoverpa zea. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 6, n. 1, p. 128-130, 2007.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 42 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. |
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Afiliação: |
Igor Henrique Sena da Silva, Universidade Estadual Paulista; Isabel Goméz, Universidad Nacional Autónoma de México; Jorge Sánchez, Universidad Nacional Autónoma de México; Diana L. Martínez de Castro, Universidad Nacional Autónoma de México; FERNANDO HERCOS VALICENTE, CNPMS; Mario Soberón, Universidad Nacional Autónoma de México; Ricardo Antonio Polanczyk, Universidade Estadual Paulista; Alejandra Bravo, Universidad Nacional Autónoma de México. |
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Título: |
Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, San Francisco, v. 13, n. 12, e0207789, 2018. |
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DOI: |
10.1371/journal.pone.0207789 |
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Idioma: |
Inglês |
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Conteúdo: |
Helicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars. MenosHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesti... Mostrar Tudo |
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Thesagro: |
Praga de Planta; Proteína; Toxina. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188667/1/Identification-midgut.pdf
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Marc: |
LEADER 02401naa a2200253 a 4500
001 2101792
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0207789$2DOI
100 1 $aSILVA, I. H. S. da
245 $aIdentification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera$bNoctuidae) that bind to Cry1Ac toxin.$h[electronic resource]
260 $c2018
520 $aHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars.
650 $aPraga de Planta
650 $aProteína
650 $aToxina
700 1 $aGOMÉZ, I.
700 1 $aSÁNCHEZ, J.
700 1 $aCASTRO, D. L. M. de
700 1 $aVALICENTE, F. H.
700 1 $aSOBERÓN, M.
700 1 $aPOLANCZYK, R. A.
700 1 $aBRAVO, A.
773 $tPlos One, San Francisco$gv. 13, n. 12, e0207789, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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