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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
23/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
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Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
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Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
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ISSN: |
1435-8603 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Thesagro: |
Semente. |
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Categoria do assunto: |
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Marc: |
LEADER 02347naa a2200193 a 4500
001 2061470
005 2017-01-23
008 2017 bl uuuu u00u1 u #d
022 $a1435-8603
100 1 $aCALVI, G. P.
245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource]
260 $c2017
520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.
650 $aSemente
700 1 $aAUD, F. F.
700 1 $aFERRAZ, I. D. K.
700 1 $aPRITCHARD, H. W.
700 1 $aKRANNER, I.
773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/01/2004 |
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Data da última atualização: |
10/04/2008 |
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Autoria: |
PIRES, C. S. S.; FONTES, E. M. G.; SUJII, E. R. |
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Título: |
Avaliação ecológica de risco de plantas geneticamente modificadas resistentes a insetos sobre inimigos naturais. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: PIRES, C. S. S.; FONTES, E. M. G.; SUJII, E. R. (Ed.). Impacto ecológico de plantas geneticamente modificadas: o algodão resistente a insetos como estudo de caso. Brasília: Embrapa Recursos Genéticos e Biotecnologia: CNPq, 2003. |
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Páginas: |
p. 85-115. |
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Idioma: |
Português |
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Palavras-Chave: |
Resistência a insetos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00688naa a2200157 a 4500
001 1184905
005 2008-04-10
008 2003 bl uuuu u00u1 u #d
100 1 $aPIRES, C. S. S.
245 $aAvaliação ecológica de risco de plantas geneticamente modificadas resistentes a insetos sobre inimigos naturais.
260 $c2003
300 $ap. 85-115.
653 $aResistência a insetos
700 1 $aFONTES, E. M. G.
700 1 $aSUJII, E. R.
773 $tIn: PIRES, C. S. S.; FONTES, E. M. G.; SUJII, E. R. (Ed.). Impacto ecológico de plantas geneticamente modificadas: o algodão resistente a insetos como estudo de caso. Brasília: Embrapa Recursos Genéticos e Biotecnologia: CNPq, 2003.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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