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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/12/2001 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To verify whether the presence of any of the cry1C. 1D, 1E and 1F genes could be associated with high toxicity against fall armyworm. Methods and Results: A sample of 60 strains from a large collections of tropical Bacillus thuringiensis (B.t.) isolates was subjected to feeding bioassay and gene-specific PCR. Positive amplification of cry-specific fragments, so confirmed by sequencing, revealed that cry1C was ubiquitous and distributed among high and low mortality classes, cry1D was underrepresented and showed no clear association to high toxicity, and cry1F was not detected. The presence of cry1E significantly correlated to high levels of insecticidal activity, as estimated by linear regression analysis. Conclusions: The PCR amplification of cry1E-specific fragments alone appears to be sufficient to identify B.t. strains with high mortality levels against tropical armyworm. Significance and Impact of the Study: The approach presented is promising as a simple and efficient method for first-tier, marker-assisted screening of environment-specific B.t. germplasm effective in controlling a single target pest. |
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Palavras-Chave: |
Armyworm; Lagarta do cartucho. |
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Thesagro: |
Controle Biológico; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01972naa a2200229 a 4500
001 1485179
005 2018-06-07
008 2001 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aAssociation of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera$bNoctuidae) larvae.$h[electronic resource]
260 $c2001
520 $aAims: To verify whether the presence of any of the cry1C. 1D, 1E and 1F genes could be associated with high toxicity against fall armyworm. Methods and Results: A sample of 60 strains from a large collections of tropical Bacillus thuringiensis (B.t.) isolates was subjected to feeding bioassay and gene-specific PCR. Positive amplification of cry-specific fragments, so confirmed by sequencing, revealed that cry1C was ubiquitous and distributed among high and low mortality classes, cry1D was underrepresented and showed no clear association to high toxicity, and cry1F was not detected. The presence of cry1E significantly correlated to high levels of insecticidal activity, as estimated by linear regression analysis. Conclusions: The PCR amplification of cry1E-specific fragments alone appears to be sufficient to identify B.t. strains with high mortality levels against tropical armyworm. Significance and Impact of the Study: The approach presented is promising as a simple and efficient method for first-tier, marker-assisted screening of environment-specific B.t. germplasm effective in controlling a single target pest.
650 $abiological control
650 $aControle Biológico
650 $aSpodoptera Frugiperda
653 $aArmyworm
653 $aLagarta do cartucho
700 1 $aSANTOS, C. G.
700 1 $aBARRETO, M. R.
700 1 $aGUIMARAES, C. T.
700 1 $aPAIVA, E.
773 $tLetters in Applied Microbiology, Oxford$gv. 32, n. 5, p. 362-367, 2001.
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
15/10/2020 |
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Data da última atualização: |
15/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. |
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Afiliação: |
LARISSA CASTRO DE REZANDE, UFLA; ANDRÉ LUIZ DE ANDRADE CARVALHO, UFLA; LÚCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; LUCAS GUEDES SILVA, FCA-UNESP; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFLA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020. |
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ISSN: |
1573-0972 |
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DOI: |
https://doi.org/10.1007/s11274-020-02882-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum. MenosAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these opt... Mostrar Tudo |
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Palavras-Chave: |
Shite mold; Submerged spores. |
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Thesagro: |
Fermentação; Fungo Para Controle Biológico; Trichoderma. |
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Thesaurus NAL: |
Biological control; Biological control agents; Bioreactors; Experimental design; Mass rearing; Molds (fungi); Solid state fermentation. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03117naa a2200397 a 4500
001 2125536
005 2020-10-15
008 2020 bl uuuu u00u1 u #d
022 $a1573-0972
024 7 $ahttps://doi.org/10.1007/s11274-020-02882-7$2DOI
100 1 $aREZENDE, L. C. de
245 $aOptimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum.$h[electronic resource]
260 $c2020
520 $aAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum.
650 $aBiological control
650 $aBiological control agents
650 $aBioreactors
650 $aExperimental design
650 $aMass rearing
650 $aMolds (fungi)
650 $aSolid state fermentation
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aTrichoderma
653 $aShite mold
653 $aSubmerged spores
700 1 $aCARVALHO, A. L. de A.
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aSILVA, L. G.
700 1 $aPINTO. Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V. de
700 1 $aMASCARIN, G. M.
700 1 $aBETTIOL, W.
773 $tWorld Journal of Microbiology & Biotechnology$gv. 36, n. 8, article 113, 2020.
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