Data/hora: 06/06/2024 13:14:09

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 10/11/2017
Data da última atualização: | 10/11/2017
Autoria: | RIBEIRO, C. A. G.; PINTO, M. de O.; MACIEL, T. E. F.; PASTINA, M. M.; GOMES, E. G. de; GUIMARAES, C. T.
Afiliação: | Carlos Alexandre Gomes Ribeiro, Universidade Federal de Viçosa; MARCOS DE OLIVEIRA PINTO, CNPMS; Talles Eduardo Ferreira Maciel, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; Everaldo Gonçalves de Barros, Universidade Católica de Brasília; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
Título: | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.
Ano de publicação: | 2017
Fonte/Imprenta: | Scientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017.
DOI: | 10.1590/1678-992x-2016-0079
Idioma: | Inglês
Conteúdo: | The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10&#8722;7 to 10&#8722;14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
Palavras-Chave: | Caracterização genética; Genotipagem.
Thesagro: | Variedade; Zea mays.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/166544/1/Universal-tail.pdf
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (27948-1) - UPC - AP - DD -  -  -  - DIS