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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/04/2015 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. |
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Afiliação: |
Pablo del Cerro; AMANDA ALVES PAIVA ROLLA SANTOS, CNPSo - Pós-graduanda; DOUGLAS FABIANO GOMES, CNPSo - Pós-graduando; BETTINA BERQUÓ MARKS, CNPSo - Pós-graduanda; FRANCISCO PÉREZ-MONTAÑO; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL; ANDRÉ SHIGUEYOSHI NAKATANI; ANTONIO GIL-SERRANO; MANUEL MEGÍAS; FRANCISCO JAVIER OLLERO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-015-1458-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Nodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis. MenosNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our p... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122208/1/regulatory-nodD1.pdf
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Marc: |
LEADER 02861naa a2200289 a 4500
001 2013361
005 2017-06-19
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-015-1458-8$2DOI
100 1 $aCERRO, P. del
245 $aRegulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation.$h[electronic resource]
260 $c2015
520 $aNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aGOMES, D. F.
700 1 $aMARKS, B. B.
700 1 $aPÉREZ-MONTAÑO, F.
700 1 $aRODRÍGUEZ-CARVAJAL, M. A.
700 1 $aNAKATANI, A. S.
700 1 $aGIL-SERRANO, A.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London, [S. l.]$gv. 16, n. 251, Mar. 2015. 13 p.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 59 | |
| 5. |  | MARTINS, M. F.; PINTO, I. S. B.; FONSECA, I.; MACHADO, M. A.; ARBEX, W. A.; SILVA, M. V. G. B. Testes de paternidade em bovinos. Parte 2: interpretação dos resultados. O Girolando, v. 14, n. 78, p. 28-29, 2011.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | WOHLRES, S.; PINTO, I. S. B.; BATISTA, R. I. T. P.; FAZA, A. P.; PAIVA, F. P.; MAFFILI, V. V.; CAMARGO, L. S. A.; VIANA, J. H. M. Are superovulation protocols realy efficient for embryo production in inbred mice lineages? Acta Scientiae Veterinariae, v. 34, s584, 2006. Supl. 1. Edição dos resumos da 20 Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | FONSECA, I.; PINTO, I. S. B.; MENDONÇA, J. F. M.; BRANDAO, H. de M.; GUIMARAES, A. S.; CARVALHO, W. A.; VICCINI, L. F.; MARTINS, M. F. Análise do perfil de expressão dos genes FOXP3 e A2M em úberes extracorpóreos em resposta à Streptococcus agalactiae. In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 4 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | PINTO, I. S. B.; FONSECA, I.; SANTOS, A. S. DE O. DOS; ARBEX, W. A.; SANTOS, K. C. L. dos; SILVA, M. V. G. B.; MARTINS, M. F. Componentes do leite e as propriedades de suas proteínas para a saude. Girolando, v. 15, n. 93, p. 12-14, 2013.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | PASSOS, L. P.; SUZUKI, L. S.; MARQUES, R.; PINTO, I. S. B.; SANTISTEVAN, G.; COUTINHO, P. R. C.; FONSECA, I.; MARTINS, M. F. Improving protein extraction from Brachiaria ruziziensis leaf samples by using a plant protease inhibitor cocktail and a chaotropic reagent. In: ASA-CSSA-SSSA International Annual Meetings, 2011, San Antonio. Abstracts...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | PAIVA, D. S.; FONSECA, I.; PINTO, I. S. B.; IANELLA, P.; CAMPOS, T. A.; CAETANO, A. R.; PAIVA, S. R.; SILVA, M. V. G. B.; MARTINS, M. F. Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in brazilian girolando cattle. Genetics and Molecular Research, v. 12, n. 3, p. 3186-3192, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | SILVA, M. V. G. B.; PINTO, I. S. B.; PAIVA, D. de S.; GUEDES, E.; ARBEX, W. A.; GUIMARAES, M. F. M. Genes candidatos de interesse na pecuária leiteira. O Girolando, Uberaba, v. 11, n. 73, p. 40-41, 2010.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | FONSECA, I.; PINTO, I. S. B.; BRITO, M. A. V. P. e; FERREIRA, M. B. D.; BRANDAO, H. de M.; GUIMARÃES, S. E. F.; MARTINS, M. F. Expressão relativa do gene IL-17 em células do leite de vacas gir infectadas com Streptococcus agalactiae. In: Congresso Brasileiro de Zootecnia, 21., 2011, Maceió. Anais...| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | GUIMARAES, M. F. M.; PAIVA, D. de S.; PINTO, I. S. B.; GUEDES, E.; ARBEX, W. A.; SILVA, M. V. G. B. Doenças genéticas em gado de leite. O Girolando, Uberaba, v. 11, n. 72, p. 20-22, 2010.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | COSTA, F. F.; BRITO, M. A. V. P. e; SOUZA, G. N. de; PEREIRA, D. B. C.; PINTO, I. S. B.; MARTINS, M. F. Efeito da temperatura das amostras de leite na concentração de cálcio solúvel e de beta-caseina: interferencia no teste de estabilidade frente ao etanol. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 66, n. 2, p. 573-578, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | FAZA, A. P.; PINTO, I. S. B.; MONTEIRO, C. M. O.; ANTUNES, G. R.; PAIVA, D. S.; GUIMARAES, M. F. M.; FURLONG, J.; PRATA, M. C. de A. Desenvolvimento de protocolos de extração de DNA genômico de Rhipicephalus (Boophilus) Microplus (Acari: ixodidae). In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | PAIVA, D. de S.; MEIRA, L. H. da R.; FONSECA, I.; PINTO, I. S. B.; ALVINO, R. M.; SILVA, M. V. G. B.; GUIMARAES, M. F. M. Diagnóstico molecular da doença hereditária BLAD em bovinos da raça Girolando. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 5., 2010, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2010. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 141.). p. 89-91.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | FURTADO, M. A. M.; COSTA, F. F.; PINTO, I. S. B.; GUIMARAES, M. F. M.; BRITO, M. A. V. P. e; PAIVA, I. M. de. Detecção da presença de leite de vaca adicionado a leite de cabra por intermétio de eletroforese "Lab-on-a-chip". In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 4., 2010, Florianópolis. Anais... Florianópolis: CBQL, 2010. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 59 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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