|
|
Registros recuperados : 291 | |
101. | | ALTAFIN, I. G.; OLIVEIRA, M. N. de; PINHEIRO, M. E. F.; GREGOLIN, A. C.; PERECMANIS, S. O mercado do leite em assentamentos de reforma agrária: acompanhamento e avaliação de ações técnicas e sociais. In: OLIVEIRA, M. N. de; XAVIER, J. H. V.; ALMEIDA, S. C. R. de; SCOPEL, E. (Ed.). Projeto Unaí: pesquisa e desenvolvimento em assentamentos de reforma agrária. Brasília, DF: Embrapa Informação Tecnológica; Planaltina, DF: Embrapa Cerrados, 2009. p. 143-181. Biblioteca(s): Embrapa Cerrados. |
| |
105. | | ANDREOTTI, R.; PINHEIRO, M. S.; SANTOS, S. A.; AZEVEDO, J. R. M.; BARROS, J. C. Observacoes sobre a preferencia termica de agua pelo jacare do Pantanal (Caiman crocodilus yacare, Daudin, 1802). In: CONGRESSO PANAMERICANO DE CIENCIAS VETERINARIAS, 15., 1996, Campo Grande. Abstracts... Campo Grande: SOMVET-MS / CRMV-MS, 1996. p.82 Biblioteca(s): Embrapa Pantanal. |
| |
108. | | BATISTA, V. G. L.; PINHEIRO, M. P. N.; SANTOS, R. C. dos; LIMA, L. M. de. Estudo da expressão de genes relacionados ao botão floral do algodoeiro por meio RT-PCR semiquantitativa. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 35 Biblioteca(s): Embrapa Algodão. |
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109. | | CHAGAS, S. F.; EVANGELISTA, W. V.; SILVA, J. de C.; PINHEIRO, M. A. Estudo da retenção e penetração de CCA na madeira de teca, visando uso como mourões tratados. Scientia Forestalis, Piracicaba, v. 43, n. 105, p. 155-165, mar. 2015. Biblioteca(s): Embrapa Florestas. |
| |
118. | | PINHEIRO, M. S.; CARDOSO, E. da C.; PIMENTEL, G. B. M. Potencial de tração de bubalinos em diversas modalidades de trabalho. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 5., 1995, Belém, PA. Resumos... Belém, PA: FCAP, Unidade de Apoio à Pesquisa, 1995. p. 32. Biblioteca(s): Embrapa Amazônia Oriental. |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
07/01/2019 |
Data da última atualização: |
27/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; MARASHIN, F. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
Afiliação: |
Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, BraZIL; VANESSA BUFFON, CNPUV; Jorge E.A. Mariath, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Felipe S. Maraschin, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Márcia Margis-Pinheiro, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
Título: |
Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.). |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Plant Science, v. 269, p. 126-135, 2018. |
Idioma: |
Inglês |
Conteúdo: |
Seedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11 |
Palavras-Chave: |
Grapevine; Overexpression; Plant plasmid; Seed; Silencing; VviAGL11. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189886/1/Malabarba-Plant-Science.pdf
|
Marc: |
LEADER 02185naa a2200265 a 4500 001 2103200 005 2019-04-27 008 2018 bl uuuu u00u1 u #d 100 1 $aMALABARBA, J. 245 $aManipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).$h[electronic resource] 260 $c2018 520 $aSeedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11 653 $aGrapevine 653 $aOverexpression 653 $aPlant plasmid 653 $aSeed 653 $aSilencing 653 $aVviAGL11 700 1 $aBUFFON, V. 700 1 $aMARIATH, J. E. A. 700 1 $aMARASHIN, F. S. 700 1 $aMARGIS-PINHEIRO, M. 700 1 $aPASQUALI, G. 700 1 $aREVERS, L. F. 773 $tPlant Science$gv. 269, p. 126-135, 2018.
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