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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, G. A.; PARENTONI, S. N.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
A search for RFLP markers to identify genes for aluminum tolerance in maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista Brasileira de Genética, Ribeirão Preto, v. 20, n. 3, p. 459-465, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8. MenosThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested f... Mostrar Tudo |
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Palavras-Chave: |
Maize; RFLP; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Milho; Zea Mays. |
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Thesaurus Nal: |
aluminum. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43456/1/Search-RFLP.pdf
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Marc: |
LEADER 02698naa a2200253 a 4500
001 1478779
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aTORRES, G. A.
245 $aA search for RFLP markers to identify genes for aluminum tolerance in maize.$h[electronic resource]
260 $c1997
520 $aThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8.
650 $aaluminum
650 $aAlumínio
650 $aMilho
650 $aZea Mays
653 $aMaize
653 $aRFLP
653 $aTolerance
653 $aTolerancia
700 1 $aPARENTONI, S. N.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tRevista Brasileira de Genética, Ribeirão Preto$gv. 20, n. 3, p. 459-465, 1997.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/11/1995 |
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Data da última atualização: |
19/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
GOMES, P. A. C.; NUERNBERG, S.; PIMENTEL NETO, M.; OLIVEIRA, G. P. de; REZENDE, H. E. B. de; ARAUJO, J. L. de B.; MELLO, R. P. de. |
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Afiliação: |
PLINIO ANTONIO COSTA GOMES, SECRETARIA DE AGRICULTURA E ABASTECIMENTO DE NITERÓI; SILVINO NUERNBERG, IPEACS; MANOEL PIMENTEL NETO, IPEACS; GILSON PEREIRA DE OLIVEIRA, EMBRAPA - UEPAE de São Carlos, São Carlos, SP.; HUGO EDISON BARBOZA DE REZENDE, UFRRJ; JOSÉ LUIZ DE BARROS ARAÚJO, UFRRJ; RUBENS PINTO DE MELLO, UFRRJ. |
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Título: |
Biologia da Lymnaea columella say 1817 (Mollusca, Gastropoda, Basommatophora, Lymnaeidae). |
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Ano de publicação: |
1975 |
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Fonte/Imprenta: |
Arquivos Museu Nacional, Rio de Janeiro, v.55, p.67-70, 1975. |
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Idioma: |
Português |
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Notas: |
Resumo. |
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Conteúdo: |
Foi estudado o ciclo da Lymnaea columella em condições de laboratório. Os dados foram coletados em diferentes locais do Estado do Rio de Janeiro. |
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Palavras-Chave: |
Biology; Lymnaea columella. |
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Thesagro: |
Biologia. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/121741/1/PROCI-1975.00003.pdf
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Marc: |
LEADER 00859naa a2200241 a 4500
001 1042558
005 2021-07-19
008 1975 bl uuuu u00u1 u #d
100 1 $aGOMES, P. A. C.
245 $aBiologia da Lymnaea columella say 1817 (Mollusca, Gastropoda, Basommatophora, Lymnaeidae).$h[electronic resource]
260 $c1975
500 $aResumo.
520 $aFoi estudado o ciclo da Lymnaea columella em condições de laboratório. Os dados foram coletados em diferentes locais do Estado do Rio de Janeiro.
650 $aBiologia
653 $aBiology
653 $aLymnaea columella
700 1 $aNUERNBERG, S.
700 1 $aPIMENTEL NETO, M.
700 1 $aOLIVEIRA, G. P. de
700 1 $aREZENDE, H. E. B. de
700 1 $aARAUJO, J. L. de B.
700 1 $aMELLO, R. P. de
773 $tArquivos Museu Nacional, Rio de Janeiro$gv.55, p.67-70, 1975.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Origem |
Tipo/Formato |
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