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| Registros recuperados : 38 | |
| 4. |  | PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P. An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 2, p. 150-158, abr./jun. 2017.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | VALDISSER, P. A. M. R.; ZUCCHI, M. I.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P. Caracterização do germoplasma de feijoeiro comum com base em marcadores DArt-Seq. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | DEUS, K. E. de; PEREIRA, W. J.; MITSUZONO, S. T.; VIANELLO, R. P.; LANNA, A. C. Sistema de defesa antioxidativo de plantas de arroz de terras altas (Oryza sativa L.) cultivadas sob deficiência hídrica. In: SEMINÁRIO JOVENS TALENTOS, 7., 2013, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2013. p. 93. (Embrapa Arroz e Feijão. Documentos, 292). Pôster - pós-graduação.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | ABREU, F. R. M.; DEUS, K. E. de; PEREIRA, W. J.; SILVEIRA, R. D. D.; VIANELLO, R. P.; BRONDANI, C. Expression of rice genes homologous of Arabidopsis genes previously related to drought tolerance. Australian Journal of Crop Science, v. 10, n. 9, p. 1266-1271, 2016.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 13. | | PEREIRA, W. J.; SIQUEIRA, B. dos S.; DEUS, K. E. de; FERNANDES, K. F.; BASSINELLO, P. Z.; VIANELLO, R. P. Determinação das condições de qRT-PCR para genes envolvidos no fenômeno hard-to-cook em feijão carioca. In: SEMINÁRIO JOVENS TALENTOS, 6., 2012, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2012. p. 33. (Embrapa Arroz e Feijão. Documentos, 275).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | SIQUEIRA, B.; PEREIRA, W. J.; BATISTA, K. A.; OOMAH, B. D.; FERNANDES, K. F.; BASSINELLO, P. Z. Influence of storage on darkening and hardening of slow- and regular-darkening carioca bean (Phaseolus vulgasris L.) genotypes. Journal of Agricultural Studies, v. 2, n. 2, p. 87-104, Sept. 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 15. | | ARAÚJO, R. B. de; PEREIRA, W. J.; VALDISSER, P. A. M. R.; LANNA, A. C.; BRONDANI, C.; VIANELLO, R. P. Validação de genes candidatos para tolerância à seca via qPCR em um amplo grupo de genótipos de feijão submetidos à deficiência hídrica. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 111. (Embrapa Arroz e Feijão. Documentos, 309).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | PEREIRA, W. J.; MELO, A. T. de O.; VALDISSER, P. A. M. R.; BRONDANI, C.; COELHO, A. S. G.; VIANELLO, R. P. Análise ampla do genoma funcional de feijoeiro em condições de deficiência hídrica. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 108. (Embrapa Arroz e Feijão. Documentos, 309).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 17. | | DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P. Molecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought. Australian Journal of Crop Science, v. 9, n. 8, p. 744-753, 2015.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 18. | | OLIVEIRA, J. A. V. de; ABREU, F. R. M.; PEREIRA, W. J.; SILVEIRA, R. D. D.; MENDONÇA, J. A.; VIANELLO, R. P.; BRONDANI, C. Expressão de genes homólogos de Arabidopsis em arroz relacionados ao aumento do potencial produtivo. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 126. (Embrapa Arroz e Feijão. Documentos, 309).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 19. | | ARAÚJO, R. B. de; LANNA, A. C.; COELHO, G. R. C.; PEREIRA, W. J.; VALDISSER, P. A. M. R.; BRONDANI, C.; VIANELLO, R. P. Estudo da expressão e validação de genes candidatos induzidos em condições de deficiência hídrica em feijão (Phaseolus vulgaris L.). In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 42. (Embrapa Arroz e Feijão. Documentos, 311).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 20. | | VALDISSER, P. A. M. R.; PEREIRA, W. J.; MORAIS JUNIOR, O. P. de; MENDONÇA, J. A.; SARTORI, D. E. L.; LIMA, LUANN V. V. O.; BORBA, T. C. de O.; BRONDANI, C.; ZUCCHI, M. I.; VIANELLO, R. P. Análise de associação genômica ampla (GWAS) para tolerância à seca em feijoeiro comum. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 67. CONAFE| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 38 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/09/2014 |
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Data da última atualização: |
29/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P. |
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Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, BIOAGRO - UFV; TETSU SAKAMOTO, UFMG; RICARDO DIÓGENES DIAS SILVEIRA; PATRICIA FERNANDA ZAMBUSSI-CARVALHO, UFG; MARISTELA PEREIRA, UFG; GEORGIOS JOANIS PAPPAS JUNIOR, UNB; MARCOS MOTA DO CARMO COSTA, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; WENDELL JACINTO PEREIRA; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 32, p. 438-451, 2014. |
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DOI: |
10.1007/s11105-013-0651-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. MenosDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly thos... Mostrar Tudo |
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Thesagro: |
Deficiência hídrica; Feijão; Phaseolus vulgaris. |
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Thesaurus NAL: |
Abiotic stress; Beans; Water stress. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180004/1/Muller2014-Article-DifferentiallyExpressedGenesDu.pdf
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Marc: |
LEADER 02986naa a2200325 a 4500
001 1994817
005 2024-04-29
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s11105-013-0651-7$2DOI
100 1 $aMÜLLER, B. S. de F.
245 $aDifferentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.$h[electronic resource]
260 $c2014
520 $aDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding.
650 $aAbiotic stress
650 $aBeans
650 $aWater stress
650 $aDeficiência hídrica
650 $aFeijão
650 $aPhaseolus vulgaris
700 1 $aSAKAMOTO, T.
700 1 $aSILVEIRA, R. D. D.
700 1 $aZAMBUSSI-CARVALHO, P. F.
700 1 $aPEREIRA, M.
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aCOSTA, M. M. do C.
700 1 $aGUIMARÃES, C. M.
700 1 $aPEREIRA, W. J.
700 1 $aBRONDANI, C.
700 1 $aVIANELLO-BRONDANI, R. P.
773 $tPlant Molecular Biology Reporter, Athens$gv. 32, p. 438-451, 2014.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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