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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/01/2024 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, K. de; BESPALHOK FILHO, J. C.; SANTOS, T. B. dos; SOUZA, S. G. H. de; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. |
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Afiliação: |
KENIA DE CARVALHO, INSTITUTO AGRONÔMICO DO PARANÁ; JOÃO CARLOS BESPALHOK FILHO, UNIVERSIDADE FEDERAL DO PARANÁ; TIAGO BENEDITO DOS SANTOS, INSTITUTO AGRONÔMICO DO PARANÁ; SILVIA GRACIELE HÜLSE DE SOUZA, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ GONZAGA ESTEVES VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; DOUGLAS SILVA DOMINGUES, INSTITUTO AGRONÔMICO DO PARANÁ. |
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Título: |
Nitrogen starvation, salt and heat stress in coffee (coffea arabica L.): identification and validation of new genes for qPCR normalization. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Molecular Biotechnology, v. 53, n. 3, p. 315-325, 2013. |
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DOI: |
https://www.doi.org/10.1007/s12033-012-9529-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abiotic stresses are among the most important factors that affect food production. One important step to face these environmental challenges is the transcriptional modulation. Quantitative real-time PCR is a rapid, sensitive, and reliable method for the detection of mRNAs and it has become a powerful tool to mitigate plant stress tolerance; however, suitable reference genes are required for data normalization. Reference genes for coffee plants during nitrogen starvation, salinity and heat stress have not yet been reported. We evaluated the expression stability of ten candidate reference genes using geNorm PLUS, NormFinder, and BestKeeper softwares, in plants submitted to nitrogen starvation, salt and heat stress. EF1, EF1 alpha, GAPDH, MDH, and UBQ10 were ranked as the most stable genes in all stresses and software analyses, while RPL39 and RPII were classified as the less reliable references. For reference gene validation, the transcriptional pattern of a Coffea non-symbiotic hemoglobin (CaHb1) was analyzed using the two new recommended and the most unstable gene references for normalization. The most unstable gene may lead to incorrect interpretation of CaHb1 transcriptional analysis. Here, we recommend two new reference genes in Coffea for use in data normalization in abiotic stresses: MDH and EF1. |
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Thesaurus NAL: |
High temperature high pressure treatment; Leaves; Minerals; Nitrogen; Nutrition; Roots; Salinity. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02243naa a2200289 a 4500
001 2160504
005 2024-01-08
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://www.doi.org/10.1007/s12033-012-9529-4$2DOI
100 1 $aCARVALHO, K. de
245 $aNitrogen starvation, salt and heat stress in coffee (coffea arabica L.)$bidentification and validation of new genes for qPCR normalization.$h[electronic resource]
260 $c2013
520 $aAbiotic stresses are among the most important factors that affect food production. One important step to face these environmental challenges is the transcriptional modulation. Quantitative real-time PCR is a rapid, sensitive, and reliable method for the detection of mRNAs and it has become a powerful tool to mitigate plant stress tolerance; however, suitable reference genes are required for data normalization. Reference genes for coffee plants during nitrogen starvation, salinity and heat stress have not yet been reported. We evaluated the expression stability of ten candidate reference genes using geNorm PLUS, NormFinder, and BestKeeper softwares, in plants submitted to nitrogen starvation, salt and heat stress. EF1, EF1 alpha, GAPDH, MDH, and UBQ10 were ranked as the most stable genes in all stresses and software analyses, while RPL39 and RPII were classified as the less reliable references. For reference gene validation, the transcriptional pattern of a Coffea non-symbiotic hemoglobin (CaHb1) was analyzed using the two new recommended and the most unstable gene references for normalization. The most unstable gene may lead to incorrect interpretation of CaHb1 transcriptional analysis. Here, we recommend two new reference genes in Coffea for use in data normalization in abiotic stresses: MDH and EF1.
650 $aHigh temperature high pressure treatment
650 $aLeaves
650 $aMinerals
650 $aNitrogen
650 $aNutrition
650 $aRoots
650 $aSalinity
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aSANTOS, T. B. dos
700 1 $aSOUZA, S. G. H. de
700 1 $aVIEIRA, L. G. E.
700 1 $aPEREIRA, L. F. P.
700 1 $aDOMINGUES, D. S.
773 $tMolecular Biotechnology$gv. 53, n. 3, p. 315-325, 2013.
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