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| Registros recuperados : 180 | |
| 101. |  | SANTOS, T. B. dos; BABA, V. Y.; SOARES, J. D. M.; CARVALHO, K. de; BRAGHINI, M. T.; PEREIRA, L. F. P.; DOMINGUES, D. S. Efeito da ferrugem e da supressão de nitrogênio no nível transcricional do gene nitrato redutase (CaNR) em Coffea arabica L. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Biblioteca(s): Embrapa Café. |
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| 102. | | CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011.| Biblioteca(s): Embrapa Café. |
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| 103. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Application of near infrared spectroscopy for green coffee biochemical phenotyping. Journal of Near Infrared Spectroscopy, v. 22, n. 6, p. 411-421, 2014.| Biblioteca(s): Embrapa Café. |
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| 104. | | CINTRA, L. A.; SOUZA, T. B. de; PARTEKA, L. M.; BARRETO, L. M.; PEREIRA, L. F. P.; GAETA, M. L.; GUYOT, R.; VANZELA, A. L. L. An 82 bp tandem repeat family typical of 3' non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres. Genome, v.65, n. 3, p. 137-181, 2021. Date of Electronic Publication: 2021 Nov 02.| Biblioteca(s): Embrapa Café. |
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| 106. | | IVAMOTO, S. T.; POT, D.; FERREIRA, L. P.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Análise in silico da expressão gênica de citocromo p450 relacionada ao metabolismo de diterpenos em Coffea. In.: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá.| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 107. | | VIDAL, R.; FERREIRA, L. P.; LANNES, S. D.; VIEIRA, L. G. E.; MONDEGO, J.; CARAZZOLLE, M. F.; PEREIRA, G. A.; POT, D.; PEREIRA, L. F. P. Analise in silico e in vivo da diversidade nucleotidica em Coffea ssp. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Resumos expandidos. Brasília, DF: Embrapa Café.| Biblioteca(s): Embrapa Café. |
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| 108. | | PEREIRA, L. F. P.; CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; CAIXETA, E. T.; ANDRADE, A. C.; VIEIRA, L. G. E. Using a coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping approaches. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, p. 276.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 109. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil. Programme abstracts. Montpellier, France: Association for Science and Information on Coffee, 2008. PB633.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 110. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINIECK, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using a Coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 111. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINIECK, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using a Coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil.| Biblioteca(s): Embrapa Café. |
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| 112. | | KOBAYASHI, A. K.; VIEIRA, L. G. E.; BESPALHOK FILHO, J. C.; LEITE JUNIOR, R. P.; PEREIRA, L. F. P.; MOLINARI, H. B. C.; MARQUES, V. V. Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. European Journal of Plant Pathology, v. 149, n. 4, p. 865-873, 2017.| Biblioteca(s): Embrapa Agroenergia; Embrapa Café. |
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| 113. | | DIOLA, V.; LOUREIRO, M. E.; BRITO, G. G. de; CAIXETA, E. T.; ZAMBOLIN, E. M.; SAKIYAMA, N. S.; PEREIRA, L. F. P. Construção do mapa genético e físico para um gene de resistência a Hemileia vastatrix em café. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009.| Biblioteca(s): Embrapa Algodão. |
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| 114. | | CAÇÃO, S. M. B.; DINIZ, L. E. C.; SILVA. N. V.; VENIKY, F.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Construção de uma biblioteca genômica de cromossomo artificial de bactéria de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. Biotecnologia aplicada à cadeia agroindustrial do café.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 115. | | CAÇÃO, S. M. B.; DINIZ, L. E. C.; SILVA, N. V.; VENIKY, F.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Construção de uma biblioteca genômica de cromossomo artificial de bactéria de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Biblioteca(s): Embrapa Café. |
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| 116. | | PEREIRA, L. F. P.; CAÇÃO, S. M. B.; SILVA, N. V.; DINIZ, L. E. C.; ALVES, G. S. C.; VINECKY, F.; ANDRADE, A. C.; VIEIRA, L. G. E. Construction of a Coffea arabica bacterial artificial chromosome library. In: ENCUENTRO LATINO AMERICANO Y DEL CARIBE DE BIOTECNOLOGIA AGROPECUARIA: REDBIO 2007, 6., 2007, Viña del Mar, Chile. Abstracts and posters? Roma: FAO, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 117. | | SANTOS, T. B. dos; BUDZINSKI, I. G. F.; MARUR, C. J.; PETKOWICZ, C. L. O.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses. Plant Physiology and Biochemistry, v. 49, n. 4, p. 441-448, 2011.| Biblioteca(s): Embrapa Café. |
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| 118. | | SANTOS, T. B. dos; SILVA, J. C.; SOARES, J. D. M.; BABA, V. Y.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; DOMINGUES, D. S. Expressão gênica em larga escala de raizes de Coffea arabica L. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., 2015, Curitiba. Anais... Brasília, DF: Embrapa Café, 2015.| Biblioteca(s): Embrapa Café. |
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| 119. | | PAGIATTO, N. F.; IVAMOTO, S. T.; SERA, T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D.; PEREIRA, L. F. P. Evaluation of kahweol and cafestol levels in Ethiopian Coffea arabica L. accessions grown in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 217| Biblioteca(s): Embrapa Café. |
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| 120. | | PEREIRA, L. F. P.; DIOLA, V.; CAÇÃO, S. M. B.; CAIXETA, E. T.; BRITO, G. G. de; ZAMBOLIN, E. M.; LOUREIRO, M. E. Mapeamento integrado de Coffea arabica: identificação de marcadores e clones BAC para o gene de resistência a ferrugem. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 23. 2010, Bali, Indonesia.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 180 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Café. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
03/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KOBAYASHI, A. K.; VIEIRA, L. G. E.; BESPALHOK FILHO, J. C.; LEITE JUNIOR, R. P.; PEREIRA, L. F. P.; MOLINARI, H. B. C.; MARQUES, V. V. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CNPAE; LUIZ GONZAGA E. VIEIRA; JOÃO CARLOS BESPALHOK FILHO; RUI PEREIRA LEITE JUNIOR; LUIZ FILIPE P. PEREIRA; HUGO BRUNO CORREA MOLINARI, CNPAE; VIVIANI V. MARQUES. |
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Título: |
Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 149, n. 4, p. 865-873, 2017. |
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DOI: |
10.1007/s10658-017-1234-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker. MenosCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulati... Mostrar Tudo |
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Palavras-Chave: |
Bacterial disease resistance; Citrus canker; Mature tissue transformation. |
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Thesagro: |
Agrobacterium Tumefaciens. |
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Thesaurus NAL: |
Agrobacterium radiobacter; Antimicrobial peptides. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2075843
005 2024-01-03
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s10658-017-1234-5$2DOI
100 1 $aKOBAYASHI, A. K.
245 $aEnhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene.$h[electronic resource]
260 $c2017
520 $aCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker.
650 $aAgrobacterium radiobacter
650 $aAntimicrobial peptides
650 $aAgrobacterium Tumefaciens
653 $aBacterial disease resistance
653 $aCitrus canker
653 $aMature tissue transformation
700 1 $aVIEIRA, L. G. E.
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aLEITE JUNIOR, R. P.
700 1 $aPEREIRA, L. F. P.
700 1 $aMOLINARI, H. B. C.
700 1 $aMARQUES, V. V.
773 $tEuropean Journal of Plant Pathology$gv. 149, n. 4, p. 865-873, 2017.
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